Molecular Profiling of Song Nucleus HVC in the Zebra Finch

斑胸草雀宋核 HVC 的分子分析

基本信息

批准号：
7626809
负责人：
Claudio V Mello
金额：
$ 7.7万
依托单位：
OREGON HEALTH & SCIENCE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2008
资助国家：
美国
起止时间：
2008-06-01 至 2010-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7626809
关键词：
Address Adult Area Atlases Behavior Bioinformatics Biological Biological Models Brain Cell Nucleus Cells Collection Communication Corpus striatum structure Data Databases Development Experimental Models Expressed Sequence Tags Female Funding Future Gene Expression Gene Expression Regulation Genes Glass Goals Gonadal Steroid Hormones Human In Situ Hybridization Label Language Language Development Language Disorders Lead Learning Link Mediating Memory Messenger RNA Methods Molecular Molecular Probes Molecular Profiling Neurobiology Neuronal Plasticity Neurons Pathway interactions Pattern Perception Phenotype Play Population Production Property Prosencephalon Research Research Personnel Resources Role Screening procedure Sex Differentiation Songbirds Speech Spottings Synapses System Techniques Transcript base brain behavior insight male mind control molecular marker neuronal replacement neuronal survival novel public health relevance repaired research study sexual dimorphism tool vocal learning zebra finch

项目摘要

DESCRIPTION (provided by applicant): The study of the song control system of songbirds has provided fundamental insights into basic questions in neurobiology, including the neuronal basis of vocal learning and communication, the action of sex hormones on the brain and behavior, seasonal and adult neuronal plasticity, and the discovery of continued neuronal replacement in the vertebrate brain. Our long-term goal is to understand the functional organization of the song control system, and to determine how genes expressed in song control nuclei contribute to vocal learning, brain sex differentiation, and learning-related synaptic and neuronal plasticity. This goal has been greatly facilitated by the recent availability of glass-spotted microarrays containing ~18,000 unique brain-expressed cDNAs, a resource generated by an NINDS-funded collaborative consortium to study songbird neurogenomics (SoNG; NS045264). Using this resource, we have already identified ~200 genes that are enriched in the song nucleus HVC, a key nucleus involved in several aspects of song learning and production, and confirmed the patterns of expression for 20 of these using high-throughput in situ hybridization techniques. We now propose to extend this study and provide a comprehensive regional and cellular analysis of genes expressed in HVC and the songbird brain in general, and the pathways underlying molecular specializations within these nuclei. Our Specific Aims for this proposal are to: Aim 1: To identify molecular specializations of HVC in zebra finches. Aim 2: To determine the cellular expression patterns of molecular markers of HVC. Our proposed experiments represent the first steps towards generating a definitive molecular profiling of the oscine song control system and building a comprehensive, publicly available gene expression database for the zebra finch brain. We anticipate that this study will identify many novel molecular markers of specific song nuclei and uncover mechanisms involved in regulating the organization and function of the song control system of songbirds, and basic principles that control vocal learning, the basis for speech and language acquisition in humans. PUBLIC HEALTH RELEVANCE The identification of molecular markers and pathways underlying cellular specializations of specific song nuclei will likely uncover mechanisms that regulate the organization and function of the song control system of songbirds, and basic principles that control vocal learning, the basis for speech and language acquisition in humans. Our research may therefore help elucidate basic mechanisms of speech and language disorders. In a broader sense, however, our results are also likely to identify basic principles underlying brain sexual differentiation, learning and memory, and the control of brain plasticity and repair, including neuronal replacement during adulthood.

描述（由申请人提供）：对鸣禽的歌曲控制系统的研究为神经生物学中的基本问题提供了基本见解，包括声音学习和交流的神经元基础，性激素对大脑和行为，季节性和成人神经元的可行性的作用，以及在pertece脑脑中的持续神经元校正的发现。我们的长期目标是了解歌曲控制系统的功能组织，并确定在歌曲控制核中表达的基因如何有助于声音学习，脑性别分化以及与学习相关的突触和神经元可塑性。最近的玻璃斑点微阵列含有约18,000个独特的脑表达cDNA，这是由NINDS资助的协作财团生成的资源，以研究鸣禽神经基因组学（Song; NS045264）产生的资源，从而极大地促进了这一目标。使用此资源，我们已经鉴定出约200个基因，这些基因富含歌曲Nucleus HVC，这是一个参与歌曲学习和生产的多个方面的关键核，并使用了高通量的原位杂交技术证实了其中20个表达方式。现在，我们建议扩展这项研究，并对在HVC和鸣禽大脑中表达的基因以及这些核中分子专业的途径进行全面的区域和细胞分析。我们对该建议的具体目的是：目标1：确定斑马雀科中HVC的分子专业。目标2：确定HVC分子标记的细胞表达模式。我们提出的实验代表了产生Oscine歌曲控制系统确定的分子分析的第一步，并为Zebra Finch大脑建立了全面的公开基因表达数据库。我们预计，这项研究将确定许多特定歌曲核的新型分子标记，并发现涉及调节鸣禽歌曲控制系统的组织和功能的机制，以及控制声乐学习的基本原理，这是人类言语和语言获取的基础。公共卫生相关性的鉴定特定歌曲核的分子标记和途径的识别可能会发现调节鸣禽歌曲控制系统的组织和功能的机制，以及控制声乐学习的基本原理，是人类中语音和语言获取的基础。因此，我们的研究可能有助于阐明语音和语言障碍的基本机制。然而，从更广泛的意义上讲，我们的结果也可能确定大脑性别分化，学习和记忆以及对大脑可塑性和修复的控制，包括成年后的神经元替代品。

项目成果

期刊论文数量（2）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Serotonin, via HTR2 receptors, excites neurons in a cortical-like premotor nucleus necessary for song learning and production.

DOI：
10.1523/jneurosci.2281-11.2011
发表时间：
2011-09-28
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
0
作者：
Wood WE;Lovell PV;Mello CV;Perkel DJ
通讯作者：
Perkel DJ

Genomics analysis of potassium channel genes in songbirds reveals molecular specializations of brain circuits for the maintenance and production of learned vocalizations.