Transition Support for ZEBrA, A Gene Expression Brain Atlas of the Zebra Finch

对斑胸草雀基因表达脑图谱 ZEBrA 的过渡支持

基本信息

批准号：
9164865
负责人：
Claudio V Mello
金额：
$ 19.41万
依托单位：
OREGON HEALTH & SCIENCE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-09-16 至 2019-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9164865
关键词：
Address Adult Affect Animal Model Area Atlases Base of the Brain Behavior Birds Brain Cell Nucleus Chickens Cognition Cognitive Collection Columbidae Communities Comparative Study Complex Country Custom Data Databases Development Disease Ensure Evaluation Evolution Funding Funding Agency Future Gene Expression Gene Structure Genes Genetic Genetic Programming Goals Gonadal Steroid Hormones Grant Guinea Health Histology Human Image In Situ In Situ Hybridization Individual Institutes Internet Investigation Language Learning Link Maintenance Mammals Mental disorders Mission Modeling Molecular Molecular Profiling Monoclonal Antibody R24 Mus National Institute of Child Health and Human Development National Institute of General Medical Sciences National Institute of Mental Health National Institute of Neurological Disorders and Stroke National Institute on Deafness and Other Communication Disorders Neurobiology Neurons Operating System Organism Pathway interactions Pattern Phase Transition Phenotype Physiology Play Preparation Process Processed Genes Production Protocols documentation Quail Research Personnel Resolution Resources Rodent Role Serinus Services Songbirds Source Speech Structure Structure-Activity Relationship Sturnus vulgaris Synapses System United States National Institutes of Health Update Visit Work adult neurogenesis base bird song brain health cognitive skill comparative cost differential expression digital imaging experience human disease improved insight interest learned behavior male meetings nervous system disorder neurodevelopment neuronal excitability neuronal replacement nonhuman primate novel selective expression sexual dimorphism tool trait usability vocal control vocal learning web site zebra finch

项目摘要

Project Summary Avian model organisms, including songbirds (zebra finch, canaries, starlings), chicken, quail, and pigeon have contributed much to our understanding of brain function and of disorders that affect neural development, function, and cognition. However, we still lack a clear understanding of how avian brain structures, particularly those that subserve complex learned behaviors and cognition, relate to brain structures in mammals, including humans. It is also unclear how molecular brain specializations of birds relate to those of mammals. To address these gaps, we used funding from the NINDS (R03) and NIGMS (R24) to develop the Zebra finch Brain Expression Atlas (ZEBrA), currently the only in situ hybridization database of brain gene expression for any avian species. ZEBrA is a publicly accessible website that contains >2,200 high resolution digital images of brain sections from adult male zebra finches that are aligned to a reference histological atlas, and hybridized to reveal the expression of >500 genes of relevance for brain development, physiology, plasticity, and learning, including numerous linked to human diseases or to lethal or deleterious phenotypes in rodents. Many of the patterns in ZEBrA reveal differential expression across broad brain subdivisions, previously unsuspected subdomains that cannot be visualized with conventional histology, and high enrichment in nuclei within circuits underlying specific behaviors (e.g., the system that controls vocal production and learning). These patterns have yielded novel insights into molecular specializations of major regions and of specific nuclei, including the discovery of convergent molecular specializations of vocal areas that are shared between songbirds and humans. The availability of ZEBrA has had a large impact (>4,000 users, and 35,000 page views), providing an important source of genetic and neuroanatomical data for a large number of songbird and avian researchers, many funded by the NIDCD, NIMH, NINDS or NICHD. The present proposal aims to use NIGMS's Legacy mechanism to maintain ZEBrA during a transition phase where further funding is sought to ensure the long- term availability, maintenance, and possible future expansion of this unique resource. Such studies will help to further validate the use of avian model organisms for understanding the molecular basis of brain function and disorders. Planned activities consist of regular updates to ZEBrA to ensure links to important databases and compatibility to browsers are kept up-to-date, and addition of 300 already processed genes whose images are not yet available online. Included are also plans for broad dissemination of the resource, close interactions with the community to ensure its needs are met, and evaluation to maximize ZEBrA's utility and impact. Legacy funding will facilitate the transition effort, which includes the preparation of applications to an array of NIH institutes whose stated missions are in line with ZEBrA's goals, and a more limited effort to generate brain expression data for specific genes of interest to individual users but not yet on the database, on a pay for service basis.

项目摘要鸟类模型生物，包括鸣禽（斑马雀，金丝雀，八哥），鸡，鹌鹑和鸽子为我们对大脑功能和影响神经发育的疾病的理解做出了很大贡献，功能和认知。但是，我们仍然对鸟类大脑结构，尤其是尤其是那些对复杂学习的行为和认知的人，与哺乳动物的大脑结构有关，包括人类。还不清楚鸟类的分子脑专业知识与哺乳动物的分子专业相关。解决这些差距，我们使用了Ninds（R03）和Nigms（R24）的资金来发展斑马雀科大脑表达地图集（斑马），目前是任何脑基因表达的原位杂交数据库禽类。斑马是一个公开访问的网站，包含> 2200个高分辨率数字图像成年男性斑马雀科的脑部切片与参考组织学地图集排列，并杂交揭示与脑发育，生理，可塑性和学习相关的500个相关基因的表达，包括许多与人类疾病或啮齿动物中致命或有害表型相关的。许多斑马的模式揭示了跨大脑细分之间的差异表达，以前没有引起无法与常规组织学可视化的子域，并且在电路内的核中富集了很高的子域基本的特定行为（例如，控制声乐和学习的系统）。这些模式已经对主要区域和特定核的分子专业提出了新的见解，包括在鸣禽和人类。斑马的可用性产生了很大的影响（> 4,000个用户和35,000页的浏览量），提供了一个大量鸣禽和鸟类研究人员的遗传和神经解剖学数据的重要来源，许多由NIDCD，NIMH，NINDS或NICHD资助。目前的提案旨在使用Nigms的遗产在过渡阶段维持斑马的机制，寻求进一步的资金以确保长期该独特资源的术语可用性，维护以及可能的未来扩展。这样的研究将有助于进一步验证使用禽模型生物来理解大脑功能的分子基础和疾病。计划的活动包括对斑马的定期更新，以确保链接到重要数据库和与浏览器的兼容性保持最新状态，并添加300个已经处理的基因的图像是尚未在线可用。还包括广泛传播资源的计划，与社区确保满足其需求，并评估以最大化斑马的效用和影响。遗产资金将促进过渡工作，其中包括将申请准备到一系列NIH 所陈述的任务与斑马的目标一致的机构，以及更有限的努力来产生大脑付款服务基础。