A Gene Expression Brain Atlas of the Zebra Finch.

斑胸草雀的基因表达脑图谱。

基本信息

批准号：
8245200
负责人：
Claudio V Mello
金额：
$ 38.5万
依托单位：
OREGON HEALTH & SCIENCE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-04-01 至 2014-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8245200
关键词：
Adult Affect Age Animal Model Apoptosis Area Atlases Behavior Bioinformatics Birds Brain Brain Mapping Brain region Cell Cycle Regulation Cell Nucleus Cell division Chickens Comparative Genomic Analysis Complex Consultations Data Databases Development Disease Endogenous Factors Environmental Risk Factor Enzymes Expressed Sequence Tags Funding Future Gene Expression Gene Expression Profile Gene Expression Regulation Gene Family Gene Structure Gene Transfer Techniques Genes Genetic Genome Genomics Goals Gonadal Steroid Hormones Grant Growth Growth Factor Health Hormonal Hormones Human Impairment Individual Ion Channel Knock-out Knowledge Language Development Learning Libraries Link Literature Mammals Maps Modeling Molecular Molecular Genetics Molecular Profiling Mus National Human Genome Research Institute National Institute of Neurological Disorders and Stroke Nerve Growth Factor Receptors Neurobiology Neuromodulator Neurons Neurosecretory Systems Neurotransmitter Receptor Neurotransmitters Nuclear Nutritional Online Systems Organism Physiological Processes Play Production Property Receptor Cell Receptor Gene Regulation Reproductive Behavior Research Research Personnel Resources Role Screening procedure Series Shapes Site Sleep Social Behavior Songbirds Speech Structure Synapses System United States National Institutes of Health base bird song cDNA Library comparative comparative genomics deafness digital high throughput analysis improved insight interest male molecular marker neurochemistry neurogenesis neuronal excitability neuronal replacement novel photoperiodicity promoter public health relevance receptor relating to nervous system research study sex sexual dimorphism tool trait transcription factor transgene expression vocal learning vocalization zebra finch

项目摘要

DESCRIPTION (provided by applicant): Songbirds are a leading model of neurobiological research with wide implications for understanding issues of human health and disease. They are among the few organisms that have evolved vocal learning, a complex trait that provides the basis of spoken language acquisition in humans. Studies of the ontogeny of songbird vocalizations and the organization of the brain circuitry that controls song learning and production have provided unique opportunities for uncovering the neural bases of vocal learning. Songbird research has also contributed novel insights into a broad range of fundamental questions in neurobiology, such as behaviorally- regulated gene expression, sex dimorphisms and the effects of sex steroids on brain structure and function, photoperiodicity and the regulation of seasonal brain plasticity, the role of sleep in learning, the neuroendocrine regulation of reproductive and social behaviors, and neurogenesis and neuronal replacement in adulthood. To help understand how the song control circuitry and birdsong behavior are shaped by genetic mechanisms, a wide set of modern molecular and genomic resources have recently become available to songbird researchers through NIH-funded initiatives; such resources include normalized brain cDNA libraries, comprehensive annotated EST databases, microarrays, a BAC library and the completed the zebra finch genome. Such resources have been instrumental in the rapid identification of genes and gene families of neurobiological interest, the study of gene structure and regulatory domains, high- throughput analysis of gene regulation through molecular profiling studies, and comparative genomics across the major higher vertebrate groups. A key next step in making full use of these genomic resources and understanding how genes relate to brain function and behavior in songbirds is to map gene expression in the context of functional brain circuits. To achieve this goal, we propose a single Specific Aim, namely to generate a Gene Expression Brain Atlas of the Zebra Finch. Specifically we propose to map the brain expression of a large set of genes (~2,500) that are of key importance to songbird and avian brain researchers, in register with a histological atlas, and make the data available as a web-based resource. PUBLIC HEALTH RELEVANCE: This resource will have an enormous impact on further establishing songbirds as a major model organism for neurobiological research, and substantiating their relevance for human health and disease, in particular the neural basis of speech and language learning and impairment. It will provide a vastly improved molecular characterization of the zebra finch brain, allow for neurotransmitter and receptor profiling of song nuclei, and reveal how aene regulation mav be linked to the sona svstem. singing behavior and vocal learning.

描述（由申请人提供）：鸣禽是神经生物学研究的主要模型，对了解人类健康和疾病问题的影响很大。它们是少数演变出声学学习的生物之一，这是一个复杂的特征，它为人类的口语获取提供了基础。对鸣禽声音的个体发育和控制歌曲学习和生产的脑电路的组织的研究为揭示了声乐学习的神经基础提供了独特的机会。鸣鸟的研究还为神经生物学中的广泛基本问题提供了新的见解，例如受行为受调节的基因表达，性二态性以及性类固醇对脑结构和功能的影响，季节性的脑部可变性以及睡眠在学习中的作用以及神经源性的疾病，社交行为的作用，季节性的作用，生殖生殖的作用以及社会的作用。成年。为了帮助了解歌曲控制电路和鸟鸣的行为是如何通过遗传机制来塑造的，最近通过NIH资助的计划可以使用广泛的现代分子和基因组资源。这样的资源包括归一化的脑cDNA库，全面注释的EST数据库，微阵列，BAC库和完成的斑马雀科基因组。这种资源对神经生物学兴趣的基因和基因家族的快速鉴定，基因结构和调节结构域的研究，通过分子分析研究对基因调节的高吞吐量分析以及对主要较高脊椎动物的比较基因组学的高吞吐量分析。全面使用这些基因组资源并了解基因与鸣禽中的脑功能和行为如何相关的关键下一步是在功能性脑电路的背景下绘制基因表达。为了实现这一目标，我们提出了一个单一的特定目标，即生成斑马雀的基因表达脑图。具体而言，我们建议绘制一大批基因（约2,500）的大脑表达，这些基因对鸣鸟和鸟类脑研究人员至关重要，并在组织学地图集中注册，并将数据作为基于Web的资源提供。公共卫生相关性：该资源将对进一步建立鸣禽作为神经生物学研究的主要模型生物产生巨大影响，并证实其对人类健康和疾病的相关性，尤其是言语和语言学习和障碍的神经基础。它将提供斑马雀脑大脑的分子表征，可提供歌曲核的神经递质和受体分析，并揭示如何将AENE调节MAV与SONA SVSTEM链接。唱歌行为和声音学习。