Cellular and Synaptic Physiology of Auditory Processing

听觉处理的细胞和突触生理学

• 批准号: 7107951
• 负责人: Claudio V Mello
• 金额: $ 3.44万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-07-01 至 2008-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7107951
• 关键词: auditory pathways; behavioral /social science research tag; behavioral habituation /sensitization; brain morphology; communication behavior; corpus striatum; electrophysiology; experience; immunocytochemistry; learning; memory; neural information processing; neural plasticity; neurophysiology; songbirds; stimulus /response; verbal behavior; vocalization; voltage /patch clamp

DESCRIPTION (provided by applicant): This FIRCA proposal is for a collaborative research project between Dr. Ricardo M. Leao, of the Department of Physiology, School of Medicine of Ribeirao Preto, Sao Paulo, Brazil, and Dr. Claudio Mello of the Neurological Sciences Institute at the Oregon Health and Science University in Portland Oregon. Dr. Mello's NIH Grant (R01- DC02853; Gene regulation in auditory learning}, is the parent grant to this proposal. This proposal is concerned with brain circuits involved in the auditory processing of song in songbirds, more specifically the circuitry organization of the caudomedial neostriatum (NCM) in the zebra finch. The long-term goal is to elucidate the involvement of NCM in vocal communication and vocal learning. The proposed research objectives are to understand how the song processing circuits in NCM are functionally organized and whether and how these circuits are modified by experience. NCM is a major area within the auditory processing pathways of songbirds, comparable to portions of the auditory cortex of mammals. NCM neurons show robust electrophysiological responses to song and a long-lasting, stimulus- specific decrease ("habituation") of these responses upon repeated presentations of the same stimulus. This experience- dependent plasticity in NCM is a major candidate for a mechanism involved in song auditory memories. In addition, NCM neurons show marked song-induced expression of the transcription factor zenk. The established link between zenk and neuronal plasticity in mammals suggests that zenk expression in NCM is associated with synaptic plasticity in NCM circuitry. However, the information on the cellular and synaptic physiology of NCM required for testing hypotheses on the functions of NCM and song-induced zenk expression is currently not available. The proposed experiments will use electrophysiological recordings in slices in combination with morphological, tract-tracing, and immunocytochemical analysis to determine the circuit organization of NCM and to evaluate whether the habituation of NCM to song involves changes in the properties of the neuronal cells that constitute song auditory processing circuits in NCM. Results from the proposed study will contribute to our understanding of how auditory processing circuits for learned vocalizations are organized and modified by experience. Because birdsong is a learned vocal behavior, these studies will potentially help us understand how humans acquire speech, as well as possible mechanisms involved in certain speech and language disorders.

描述（由申请人提供）：该FIRCA提案是针对Ribeirao Preto医学院的Ricardo M. Leao博士，SAO Paulo，Brazil的Ricardo M. Leao博士与俄勒冈州Portland Oregon俄勒冈健康和科学大学神经科学研究所的Claudio Mello博士。 Mello博士的NIH Grant（R01- DC02853；听觉学习中的基因调节}是该建议的父母授予。 该提案与鸣禽中歌曲的听觉处理有关的脑回路涉及，更具体地说是Zebra Finch中尾状新生儿植物（NCM）的电路组织。长期目标是阐明NCM参与声带传播和声音学习。拟议的研究目标是了解NCM中的歌曲处理电路如何在功能上组织起来，以及这些电路是否以及如何通过经验修改。 NCM是鸣禽的听觉处理途径中的一个主要领域，与哺乳动物的听觉皮层相当。 NCM神经元显示出对歌曲的强大电生理反应，并在反复出现相同刺激的情况下对这些反应的持久，刺激特异性减少（“习惯”）。 NCM中这种依赖性的可塑性是涉及歌曲听觉记忆的机制的主要候选者。此外，NCM神经元显示出明显的歌曲诱导的转录因子zenk的表达。 Zenk与哺乳动物中神经元可塑性之间建立的联系表明，NCM中的Zenk表达与NCM电路中的突触可塑性有关。然而，目前尚不可用有关NCM和歌曲诱导的Zenk表达功能的假设所需的NCM细胞和突触生理的信息。 所提出的实验将在切片中使用电生理记录，结合形态学，道追踪和免疫细胞化学分析来确定NCM的回路组织，并评估NCM对歌曲的习惯是否涉及构成NCM中NCM中构成歌曲听觉处理循环的神经元细胞的变化。拟议的研究的结果将有助于我们理解如何通过经验来组织和修改学习声音的听觉处理电路。由于Birdsong是一种博学的声音行为，这些研究将有可能帮助我们了解人类如何获得言语以及某些语言和语言障碍涉及的可能机制。