CORE--TISSUE CULTURE, MICROSCOPY-IMAGING FACILITY

核心——组织培养、显微镜成像设备

• 批准号: 7342417
• 负责人: NANCY B SCHWARTZ
• 金额: $ 42.59万
• 依托单位: UNIVERSITY OF CHICAGO
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-01-01 至 2008-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7342417
• 关键词: Cell Line; Consultations; Coomassie blue; Cost Savings; Cultured Cells; Embryo; Equipment; Fluorescence; Gel; Grant; Homidium Bromide; Image; In Situ; In Situ Hybridization; Individual; Journals; Laboratories; Libraries; Messenger RNA; Microscope; Microscopy; Monoclonal Antibodies; Motor; Optics; Oranges; Preparation; Printing; Process; Proteins; Publications; Reporting; Research Personnel; Services; Techniques; Time; Tissues; foot; programs; software systems; tissue culture; tissue preparation

DESCRIPTION (provided by applicant): The Administrative and Scientific Core functions as a centralized unit to provide services to individual projects. The administrative component of the core is responsible for managing the finances of the program, including grant preparation, non competing renewals and reports, organizing program activities, including seminars, journal clubs and the Advisory Board consultations, and preparation of publications relevant to the program. The tissue culture component of the core provides centralized media preparation, supplies common cell cultures, and stock cell lines and some monoclonal antibodies in an efficient, coordinated and cost-saving manner. The microscopy/imaging facility provides investigators the opportunity to capture images from gels (DNA or protein) with built-in parameters for ethidium bromide, SYBR green, SYPRO orange, and Coomassie blue. Once the image has been captured it is available for adjustment, analysis or printing. The physical components in this facility (300 square feet) include an inverted microscope with fluorescence capabilities, optics and filters, a z-focus motor, 3-chip video system, and software that provides for macro editing and 3D processing. Over the next project period a tissue preparation/in situ facility will be developed for program investigators. These techniques are increasingly being used by Projects I, II and III, and each investigator is currently borrowing time on other investigator's equipment at a distance from their own laboratories. Because all projects use embryonic chick tissue for mRNA in situ hybridization analysis, a library of chick specific cDNAs is being shared among the investigators; most of them were constructed in their own laboratories. A continuous effort will be made for further expansion of this component of the core as needed.

描述（由申请人提供）：行政和科学核心作为一个 集中式单元为各个项目提供服务。核心的行政组成部分负责管理该计划的财务状况，包括赠款准备，非竞争性续签和报告，组织计划活动，包括研讨会，期刊俱乐部和咨询委员会咨询，以及与该计划相关的出版物的准备。核心的组织培养成分提供了集中的培养基制剂，提供常见的细胞培养物和库存细胞系以及一些单克隆抗体，以有效的，协调的和节省成本的方式。显微镜/成像设施为研究人员提供了从凝胶（DNA或蛋白质）捕获带有溴化乙锭，SYBR绿色，Sypro橙色和Coomassie Blue的内置参数的图像的机会。一旦捕获图像，就可以进行调整，分析或打印。该设施中的物理组件（300平方英尺）包括具有荧光功能，光学和过滤器的倒置显微镜，Z对焦电机，3芯片视频系统以及提供宏观编辑和3D处理的软件。在下一个项目期间，将为计划调查人员开发组织准备/原位设施。项目I，II和III越来越多地使用了这些技术，目前，每个研究人员都在借用与自己的实验室距离的其他调查员设备上的时间。因为所有项目都使用胚胎小鸡组织进行mRNA 现场杂交分析，研究人员正在共享小鸡特异性cDNA的库；他们中的大多数是在自己的实验室中建造的。根据需要，将不断努力进一步扩展核心的这一组成部分。