PROTEOMICS

蛋白质组学

基本信息

批准号：
8296118
负责人：
Dean P Edwards
金额：
$ 15.06万
依托单位：
BAYLOR COLLEGE OF MEDICINE
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/8296118
关键词：
Acetylation Affinity Affinity Chromatography Albumins Aliquot Amino Acid Sequence Amino Acids Ammonium Sulfate Antibodies Antibody Affinity Antibody Formation Antigens Apoptosis Applications Grants Area Award Bacteria Baculoviruses Binding Binding Proteins Binding Sites Biological Assay Biological Markers Biometry Bioreactors Biotin Biotinylation Blood capillaries Budgets Buffers C-terminal CD8B1 gene Cancer Biology Cancer Center Cancer Center Support Grant Cattle Cell Culture Techniques Cell Extracts Cell Line Cells Chemistry Chimeric Proteins Chromatography Color Complementary DNA Complex Computer software Consult Consultations Coomassie blue Coupled Coupling Culture Media Custom Cyclization Cytolysis Data Data Analyses Data Reporting Data Set Databases Denmark Detection Devices Diagnosis Diamond Dimensions Dissociation Dyes Enzyme-Linked Immunosorbent Assay Epitopes Equipment Equipment and supply inventories Eye Faculty Fiber Fingerprint Fluorescence Fractionation Gel Genes Genomics Glycoproteins Goals Gold Grant Growth Factor Height High Pressure Liquid Chromatography Human Resources Hybridomas Hybrids Immune response Immunoblotting Immunoglobulin G Immunohistochemistry Immunoprecipitation In Vitro Inbred BALB C Mice Infection Injection of therapeutic agent Insecta Institution Investments Ion-Exchange Chromatography Procedure Ions Label Laboratories Laboratory culture Lasers Ligands Lipids Liquid substance Logistics Malignant Neoplasms Maps Marker Discovery Mass Spectrum Analysis Measurement Mediating Medicine Membrane Metabolic Marker Methods Methylation Mitogen-Activated Protein Kinases Modeling Modification Molecular Monoclonal Antibodies Multiple Myeloma Mus N-terminal Nature Nitrogen Noise Nuclear Extract Nucleopolyhedrovirus Organization administrative structures Oryctolagus cuniculus Pathway interactions Patients Peptide Hydrolases Peptide Sequence Determination Peptide Synthesis Peptide antibodies Peptide/MHC Complex Peptides Periderm Phase Phosphoproteins Phosphorylation Phosphorylation Site Plaque Assay Plasma Plasmids Post-Translational Modification Site Post-Translational Protein Processing Precipitation Preparation Prevention Procedures Process Production Protein Analysis Protein Array Protein Chemistry Protein Databases Protein Sequence Analysis Proteins Proteome Proteomics Proteomics Shared Resource Quality Control RNA Splicing Recombinant Proteins Recombinants Research Research Personnel Resolution Resource Sharing Resources Robot Running Sampling Scanning Screening procedure Secondary to Sepharose Serum Serum Proteins Services Set protein Shuttle Vectors Signal Pathway Signal Transduction Silver Staining Site Slide Sodium Dodecyl Sulfate-PAGE Solutions Source Specificity Spectrometry Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Spleen Spottings Stable Isotope Labeling Staining method Stains Streptavidin Supervision Suspension Culture System T-Cell Receptor T-Lymphocyte Techniques Technology Testing Time Tissues Training Translational Research Translations Trypsin Tube Two-Dimensional Gel Electrophoresis UV sensitive Universities Validation Vendor Viral Wages Writing amidation base cancer therapy capillary college cooking cost effective cyanine dye 5 cytokine data acquisition detector experience gel electrophoresis genetic regulatory protein homologous recombination immunocytochemistry informatics shared resource instrument instrumentation interest liquid chromatography mass spectrometry mass spectrometer meetings monoclonal antibody production monomer mouse model myristoylation nano nano-electrospray new technology novel polyvinylidene fluoride pre-clinical pressure protein complex protein expression protein profiling recombinant virus reversed phase chromatography sample collection tool tumor two-dimensional validation studies vector viral DNA

项目摘要

The goal of this shared resource is to provide investigators with cost effective state-of-the-art instrumentation, and specialized expertise for analysis of proteomes with the goal of identifying novel protein biomarkers with applications for prevention, diagnosis and treatment of cancer. Because of the extremely diverse nature of proteins through post-translation modifications (PTMs), secretion, and RNA splicing coupled with their wide dynamic range of expression, no single platform exists for differential analysis of global protein expression. This Shared Resource therefore combines and integrates the instrumentation and expertise of several different faculty co-directors under a single organizational unit directed by Dr. Dean P. Edwards. Through a combined investment by the Institution and the Dan L. Duncan Cancer Center of $1.7M in equipment, start-up salary for new staff and operational support, the Proteomics Shared Resource (PSR) has expanded its technological capabilities and services and has grown tremendously to become one of the heaviest utilized Resources of the Cancer Center. The PSR is currently staffed by four co-directors (Drs Jun Qin, Richard Cook, Shixia Huang, Eastwood Leung) and six highly trained technical staff. New technologies and services developed since the CCSG submission in 2006 include non-mass spectrometry-based protein profiling platforms such as the Luminex fluorescence bead system, two dimensional liquid and gel electrophoresis and antibody/peptide arrays; mass spectrometry identification and PTM analysis of endogenous protein complexes isolated from cells, quantitative mass spectrometry protein profiling by SILAC (stable isotope labeling with heavy amino acids in cells) and enhanced throughput production of monoclonal antibodies (MAbs). These new technologies and services, together with the previously established technologies of protein chemistry, MALDI-TOF identification and PTM analysis of proteins, baculovirus expression of recombinant proteins and conventional hybridoma/MAb production, have created a highly comprehensive Proteomic Shared Resource capable of facilitating both protein biomarker discovery research and translational validation studies through custom antibody production. We will continue to expand and meet the needs of the Cancer Center through shared instrument grant applications and by providing expertise and new leading edge proteomic technologies as the field rapidly evolves.

该共享资源的目的是为研究人员提供具有成本有效的最先进的仪器，并为蛋白质组分析的专业知识提供了专业知识，目的是识别新型蛋白质生物标志物，并应用于预防，诊断和治疗癌症。由于蛋白质通过翻译后修饰（PTM），分泌和RNA剪接的性质极为多样化，再加上其广泛的动态表达范围，因此没有一个平台可用于对全局蛋白表达的不同分析。因此，该共享资源结合并整合了由Dean P. Edwards博士指导的单个组织部门的几个不同教师联合主管的工具和专业知识。通过该机构和Dan L. Duncan癌症中心的共同投资，设备为170万美元，新员工的启动工资和运营支持，蛋白质组学共享资源（PSR）扩大了其技术能力和服务，并已大大发展，成为利用癌症中心最重的资源之一。 PSR目前由四个联合主任（Drs Jun Qin，Richard Cook，Shixia Huang，Eastwood Leung）和六名训练有素的技术人员组成。自2006年CCSG提交以来，开发的新技术和服务包括基于非质谱的蛋白质分析平台，例如Luminex荧光珠系统，二维液体和凝胶电泳以及抗体/肽阵列；从细胞中分离出的内源性蛋白复合物的质谱鉴定和PTM分析，通过SILAC（稳定的同位素标记在细胞中用重氨基酸稳定的同位素标记）进行定量的质谱蛋白分析，并增强了吞吐量的生产单克隆抗体（mAb）。这些新技术和服务以及先前建立的蛋白质化学技术，MALDI-TOF鉴定和PTM蛋白质分析，重组蛋白质的杆状病毒表达以及传统的杂交瘤/MAB生产，创造了一种高度全面的蛋白质组织共享资源，能够通过定制研究蛋白质生物标记研究促进蛋白质生物标记研究。随着领域的迅速发展，我们将通过共享的工具赠款应用程序以及提供专业知识和新的前沿蛋白质组学技术来扩大和满足癌症中心的需求。