MYOCARDIAL INTERCALATED DISKS

心肌间盘

• 批准号: 7610338
• 负责人: STEPHEN C ARMSTRONG
• 金额: $ 0.37万
• 依托单位: UNIVERSITY OF SOUTH DAKOTA
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-05-01 至 2008-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7610338
• 关键词: Cardiac Myocytes; Co-Immunoprecipitations; Communication; Complex; Computer Retrieval of Information on Scientific Projects Database; Confocal Microscopy; Connexin 43; Dilated Cardiomyopathy; Funding; Grant; Hamsters; Institution; Intercalated disc; Mediating; Modeling; Myocardial; Myocardial Ischemia; Oryctolagus cuniculus; PAK2 kinase; Phosphorylation; Protein Tyrosine Kinase; Protein phosphatase; Proteins; Research; Research Personnel; Resources; Source; United States National Institutes of Health; protein protein interaction

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This project will examine the hypothesis that a protein complex is assembled at the myocardial intercalated disk. The proposed protein complex is composed of the tyrosine kinases, ErbB2 and Src; protein phosphatases 1/ 2 and the p21 activated kinases, PAK1 and PAK2. This complex regulates the phosphorylation of connexin 43, a protein that mediates the ionic and electrical communication between adjacent cardiomyocytes. This hypothesis will be examined by confocal microscopy and co-immunoprecipitation, to assess protein- protein interactions within this complex. This study will utilize a rabbit of myocardial ischemia and a hamster model of dilated cardiomyopathy to examine this hypothesis.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 该项目将检验蛋白质复合物在心肌闰盘处组装的假设。所提出的蛋白质复合物由酪氨酸激酶、ErbB2 和 Src 组成；蛋白磷酸酶 1/ 2 和 p21 激活激酶 PAK1 和 PAK2。该复合物调节连接蛋白 43 的磷酸化，连接蛋白 43 是一种介导相邻心肌细胞之间离子和电通讯的蛋白质。该假设将通过共聚焦显微镜和免疫共沉淀进行检验，以评估该复合物内的蛋白质-蛋白质相互作用。本研究将利用心肌缺血的兔子和扩张型心肌病的仓鼠模型来检验这一假设。