SD COBRE: CELL IMAGING CORE

SD COBRE：细胞成像核心

• 批准号: 7381828
• 负责人: STEPHEN C ARMSTRONG
• 金额: $ 9.46万
• 依托单位: UNIVERSITY OF SOUTH DAKOTA
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-01 至 2007-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7381828
• 关键词: SD; COBRE; CELL; IMAGING; CORE

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. To allow Dr. Faqian Li to focus more on his project, Dr. Steven Armstrong was recently appointed Director of this core late in year 3. Dr. Armstrong has extensive experience in confocal microscopy and his office is located directly across the hall from the confocal room. Dr. Armstrong also spearheaded an NIH equipment grant for a new confocal microscope, which has been resubmitted in year 4. Proposed projects 1, 2, and 3 require confocal microscopy. The confocal microscope was updated with new software. The old computer for the confocal system is now a working station to process images acquired by the confocal system. We have extensively investigated the translocation of G-protein coupled receptor kinases and focal adhesion kinase during cardiac hypertrophy and failure. The cellular distribution of signaling molecules in cultured cardiac myocytes transfected with adenoviral constructs has been analyzed. We established an H-E staining station. We also provide frozen sectioning for PIs. The imaging core also provides technical training and expertise for investigators doing their own confocal microscopy.

该子项目是利用 NIH/NCRR 资助的中心拨款提供的资源的众多研究子项目之一。子项目和研究者 (PI) 可能已从另一个 NIH 来源获得主要资金，因此可以在其他 CRISP 条目中得到体现。列出的机构是中心的机构，不一定是研究者的机构。为了让李法前博士能够更加专注于他的项目，史蒂文·阿姆斯特朗博士最近在第三年末被任命为该核心的主任。阿姆斯特朗博士在共焦显微镜方面拥有丰富的经验，他的办公室位于共焦显微镜大厅的正对面房间。阿姆斯特朗博士还带头为新型共焦显微镜提供了 NIH 设备拨款，该拨款已于第四年重新提交。拟议项目 1、2 和 3 需要共焦显微镜。共焦显微镜已使用新软件进行了更新。共焦系统的旧计算机现在是处理共焦系统获取的图像的工作站。我们广泛研究了心脏肥大和衰竭期间 G 蛋白偶联受体激酶和粘着斑激酶的易位。已分析了转染腺病毒构建体的培养心肌细胞中信号分子的细胞分布。我们建立了H-E染色站。我们还为 PI 提供冰冻切片。成像核心还为研究人员进行自己的共焦显微镜检查提供技术培训和专业知识。