SD COBRE: CELL IMAGING CORE

SD COBRE：细胞成像核心

• 批准号: 8168339
• 负责人: STEPHEN C ARMSTRONG
• 金额: $ 12.13万
• 依托单位: SANFORD RESEARCH/USD
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8168339
• 关键词: Appearance; Cardiovascular system; Cells; Centers of Research Excellence; Computer Retrieval of Information on Scientific Projects Database; Computers; Confocal Microscopy; Dyes; Enzyme Activation; Fluorescence; Funding; Grant; Growth; Image; Institution; Life; Microscope; Nuclear; Physiological; Proteins; Research; Research Institute; Research Personnel; Resources; Series; Source; Staining method; Stains; Time; Tissue Sample; United States National Institutes of Health; cellular imaging; cyanine dye 5; flexibility; in vivo; instrument; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In FY2006, the Imaging Core maintained the growth in confocal microscopy utilization that was described for FY2005. A major advancement in the capabilities of the Core in FY2006 derived from the acquisition of the Olympus FluoView 1000 confocal microscope which is currently located in the facilities of the Cardiovascular Research Institute. This new instrument offers all the functions of the FVX microscope that was originally included within the COBRE grant. The FV1000 automates these functions with computer control over the microscope and confocal acquisition parameters. The FV1000 allows acquisition from three fluorescence channels, permitting an assessment of the co-localization of three proteins within a cellular or tissue sample. These three fluorescence channels can be assigned to a wide variety of cellular dyes with excitation wavelengths in a range from 405nm (e.g. the nuclear stain DAPI) to 675nm (Cy5). This broad range of dye options offers investigators the opportunity to utilize the fluoro-chrome that provides the highest level of sensitivity and selectivity for their studies. This level of flexibility is permitted by the precise definition of excitation and emission wavelengths that is provided by the FV1000. The Core also installed a microscope mounted, physiologic chamber that will permit the acquisition of confocal images from living cells subjected to a variety of experimental conditions over a series of time points. These experiments can include fluorescent substrates that are micro-injected into viable cells. The cellular localization of enzyme activation can be determined in vivo by the appearance of fluorescence subsequent to enzyme activation.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此，可以在其他清晰的条目中表示。列出的机构是 对于中心，这不一定是调查员的机构。 在2006财年，成像核维持了2005财年描述的共聚焦显微镜利用率的增长。 2006财年核心能力的重大进步源自目前位于心血管研究所设施中的Olympus Fluoview 1000共聚焦显微镜。该新仪器提供了最初包含在鞋底赠款中的FVX显微镜的所有功能。 FV1000通过计算机控制显微镜和共聚焦采集参数来自动化这些功能。 FV1000允许从三个荧光通道中获取，从而评估了细胞或组织样品中三种蛋白的共定位。这三个荧光通道可以分配给各种细胞染料，其激发波长在405nm（例如核染色DAPI）到675nm（CY5）范围内。这种广泛的染料选项为研究人员提供了利用氟粉红色的机会，该氟粉红色为他们的研究提供了最高水平的敏感性和选择性。 FV1000提供的激发和发射波长的精确定义可以允许这种灵活性。 核心还安装了一个安装的显微镜生理室，该室将允许在一系列时间点上从生物细胞中获取各种实验条件的共聚焦图像。这些实验可以包括将荧光底物显微注射到可行的细胞中。可以通过酶激活后的荧光出现在体内确定酶激活的细胞定位。