Biomedical National Elemental Imaging Resource (BNEIR)

生物医学国家元素成像资源 (BNEIR)

• 批准号: 10191567
• 负责人: Brian P Jackson
• 金额: $ 46.42万
• 依托单位: DARTMOUTH COLLEGE
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-05-01 至 2026-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10191567
• 关键词: Ablation; Aluminum; Alzheimer' s Disease; Antibodies; Area; Arsenic; Arsenicals; Basic Science; Biochemical; Biological Markers; Biological Process; Biology; Brain; Cell Cycle Regulation; Cell physiology; Cells; Centers of Research Excellence; Chemicals; Coenzymes; Collaborations; Communities; Computer software; Consult; Contrast Media; Copper; Custom; Cytometry; Data Analyses; Detection; Diagnostic; Disease; Doctor of Philosophy; Drug Delivery Systems; Drug Targeting; E-learning; Elements; Ensure; Fees; Fluorescence; Fostering; Foundations; Funding; Gadolinium; Goals; Health; Hepatolenticular Degeneration; Homeostasis; Human; Human Resources; Human body; Image; Imaging Techniques; Immunity; Immunohistochemistry; Inductively Coupled Plasma Mass Spectrometry; Infrastructure; Institution; International; Internet; Iron; Isotopes; Knowledge; Laboratories; Lasers; Life; Lifting; Link; Location; Magnetic Resonance Imaging; Metals; Mission; Molecular; National Institute of General Medical Sciences; Online Systems; Organ; Organism; Performance; Persons; Platinum Compounds; Prevention; Process; Proteins; Reproduction; Research; Research Personnel; Research Project Grants; Resource Sharing; Resources; Roentgen Rays; Role; Science; Scientist; Selenium; Senile Plaques; Services; Spatial Distribution; Spectrometry, Mass, Secondary Ion; Spottings; Structure; Synchrotrons; System; Techniques; Time; Tissues; Trace Elements; Training; Training Programs; United States National Institutes of Health; Vacuum; Visit; Work; Zinc; base; beamline; bioimaging; biological systems; biomedical facility; biomedical resource; cancer therapy; cofactor; college; data acquisition; data handling; detection limit; disease diagnosis; drug efficacy; experience; experimental study; human disease; improved; industry partner; innovation; instrumentation; mass spectrometer; medical schools; meetings; metalloenzyme; nanoparticle; programs; repaired; response; software development; success; synchrotron radiation; tool; webinar

Up to 60 chemical elements can be detected in the human body, about 25 of which are essential to life. Elements such as iron (Fe), zinc (Zn), copper (Cu), and selenium (Se) are constituents of enzyme cofactors required for a wide range of processes from cell cycle regulation, reproduction, structural repair, and immunity. Dysregulation of these elements is a hallmark of disease. For instance, aluminum (Al), Fe, Cu and Zn accumulation in amyloid plaques in the brain are associated with Alzheimer’s disease and dysregulation of Cu causes Menkes and Wilson’s disease. Chemical elements are also diagnostic tools and chemotherapeutics; gadolinium (Gd) is the most common magnetic resonance imaging contrast agent, metal-base nanoparticles show promise for targeted drug delivery and platinum (Pt) and arsenic (As) compounds are used in cancer treatment. Invariably, knowing the spatial distribution of an element in an organ, tissue or cell is essential to fully investigate mechanisms of disease or the efficacy of drug delivery. Hence, elemental imaging at the micron scale is essential to further the NIGMS goal to support basic research that increases our understanding of biological processes and lays the foundation for advances in disease diagnosis, treatment, and prevention. Current analytical resources for biomedical elemental imaging are either over-subscribed (synchrotron X-ray fluorescence), cover only a limited range of elements, are performed under a vacuum or not readily scalable to imaging at the mm scale. Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) is a laboratory-based elemental imaging (elemental imaging) technique that it is increasingly being applied to biomedical applications with comparable or lower detection limits than other elemental imaging techniques and, potentially, easier access and operability. To date there are no elemental imaging LA-ICP-MS user resources in the US. The Dartmouth Trace Element Analysis Core is an established and highly regarded shared resource that supports researchers throughout the US, is staffed by experienced researchers in elemental imaging and ICP-MS, uses state-of-the-art instrumentation and has excellent links to industry partners. Our aims are to expand upon our current ad hoc elemental imaging service to establish an elemental imaging user facility; the Biomedical National Elemental Imaging Resource (BNEIR), which will accelerate and simplify access for biomedical researchers to instrumentation, expertise, web-based and in-person training, after-visit support, software and will foster a dynamic community for elemental imaging users. We will actively promote the elemental imaging within the larger NIGMS community of scientists through webinars, seminars, attendance at national and international meetings and actively seeking out NIH researchers currently pursuing relevant research projects.

人体内可检测到多达 60 种化学元素，其中约 25 种是生命必需的元素，如铁 (Fe)、锌 (Zn)、铜 (Cu) 和硒 (Se) 是酶辅助因子的成分。这些元素的失调是细胞周期调节、繁殖、结构修复和免疫等一系列过程所必需的，例如，铝 (Al)、铁、铜和锌在淀粉样斑块中积累。大脑与阿尔茨海默病有关，铜的失调会导致门克斯病和威尔逊病。化学元素也是诊断工具和化疗药物；钆 (Gd) 是最常见的磁共振成像造影剂，金属纳米颗粒有望用于靶向药物输送。铂 (Pt) 和砷 (As) 化合物通常用于癌症治疗，了解器官、组织或细胞中元素的空间分布对于全面研究疾病或疾病的机制至关重要。因此，微米级的元素成像对于推进 NIGMS 的目标至关重要，以支持基础研究，增加我们对生物过程的理解，并为疾病诊断、治疗和预防的进展奠定基础。生物医学元素成像要么被过度订阅（同步加速器 X 射线荧光），仅涵盖有限范围的元素，要么在真空下进行，要么不易扩展到毫米级成像。 (LA-ICP-MS) 是一种基于实验室的元素成像（元素成像）技术，它越来越多地应用于生物医学应用，其检测限与其他元素成像技术相当或更低，并且可能更容易访问和操作。美国没有元素成像 LA-ICP-MS 用户资源 达特茅斯痕量元素分析核心是一个成熟且备受推崇的共享资源，为全美国的研究人员提供支持，由元素成像和 ICP-MS 领域经验丰富的研究人员组成，使用。我们拥有最先进的仪器，并与行业合作伙伴建立了良好的联系，我们的目标是扩展我们当前的临时元素成像服务，建立元素成像用户设施，这将加速生物医学国家元素成像资源（BNEIR）的发展。并简化生物医学研究人员对仪器、专业知识、基于网络和现场培训、访问后支持、软件的访问，并将为元素成像用户培育一个充满活力的社区，我们将在更大的 NIGMS 社区内积极推广元素成像。科学家通过网络研讨会、研讨会、参加国内和国际会议以及积极寻找目前正在进行相关研究项目的 NIH 研究人员。