Core B: Research Support Core

核心 B：研究支持核心

• 批准号: 7792454
• 负责人: Brian P Jackson
• 金额: $ 14.84万
• 依托单位: DARTMOUTH COLLEGE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7792454
• 关键词: Acids; Analytical Chemistry; Arsenic; Arts; Biological; Biological Markers; Biological Preservation; Chemicals; Collaborations; Communities; Core Facility; Coupled; Detection; Doctor of Philosophy; Ecosystem; Educational Curriculum; Educational process of instructing; Elements; Ensure; Epidemiology; Experimental Designs; Fee-for-Service Plans; Food Chain; Food Webs; Gas Chromatography; Goals; Hair; Hand; Health; Homeostasis; Human; Individual; Isotopes; Liquid Chromatography; MMA(III); Mass Spectrum Analysis; Mercury; Metals; Methodology; Methods; Methylmercury Compounds; Mission; Plants; Plasma; Poison; Protocols documentation; Research; Research Personnel; Research Project Grants; Research Support; Resources; Sampling; Seafood; Services; Structure of nail of toe; Students; Techniques; Toxic effect; Trace Elements; Trace metal; Training; Urine; Weight; Work; analytical method; arsenobetaine; base; cost; experience; graduate student; improved; instrumentation; member; method development; milligram; novel; programs; success; toxic metal

The primary goal of the Trace Element Analysis (TEA) Core (Core B) is to provide specific analytical services and analytical expertise to Dartmouth SBRP researchers to allow them to successfully complete the aims of their individual projects. Additionally, the TEA Core strives to be at the forefront of (mission-related) method development that augments the themes of the Dartmouth SBRP projects and advances these projects by providing analytical advances such as lower detection limits, quantification of an as yet unmeasured metal species, or the novel application of an analytical methodology. The TEA Core utilizes state of the art analytical instrumentation based on inductively coupled plasma mass spectrometry (ICP-MS) to provide low level determinations of trace elements in a variety of biological and environmental matrices. The TEA Core also provides speciation analysis for arsenic and mercury by liquid chromatography and gas chromatography coupled to ICP-MS, respectively. The chemical form (species) of arsenic or mercury in a sample ultimately determines the toxicity of that element, hence speciation information is essential in assessing the human health effects of these ubiquitous contaminant elements. The TEA Core provides toenail and hair analysis to assess the exposure of an individual to trace metals. Methods have been developed to quantify six chemical species of arsenic in human urine; these species range from arsenobetaine, a non-toxic form of arsenic that people are routinely exposed to through seafood, to monomethlarsenous acid, MMA(III), an extremely toxic chemical form of arsenic. The TEA Core also provides methodology to speciate mercury at extremely low levels and in very small sample masses. Our GC-ICP-MS methods can detect less than 1 picogram of mercury and can determine mercury species in sub milligram sample weights. Methylmercury is an extremely toxic form of mercury that bioaccumulates through the food chain, hence it is essential to be able to determine this mercury species at very low levels. The TEA Core continues to push the boundary of low level trace element detection to develop and validate methods for the determination of trace elements or elemental species in biological samples with the goal of providing Dartmouth SRBP researchers the information they need to make better predictive decisions about human and ecosystem health.

痕量元素分析 (TEA) 核心（核心 B）的主要目标是提供特定的分析服务 为达特茅斯 SBRP 研究人员提供分析专业知识，使他们能够成功完成以下目标： 他们的个人项目。此外，TEA 核心致力于走在（任务相关）方法的最前沿 增强达特茅斯 SBRP 项目主题并通过以下方式推进这些项目的开发 提供分析进步，例如较低的检测限、尚未测量的金属的量化 物种，或分析方法的新颖应用。 TEA Core 采用最先进的技术 基于电感耦合等离子体质谱 (ICP-MS) 的分析仪器可提供低 各种生物和环境基质中微量元素的水平测定。 TEA 核心 还通过液相色谱和气相色谱法对砷和汞进行形态分析 分别与 ICP-MS 耦合。样品中砷或汞最终的化学形态（种类） 决定了该元素的毒性，因此形态信息对于评估人类的毒性至关重要 这些普遍存在的污染物元素对健康的影响。 TEA Core 提供脚趾甲和毛发分析 评估个人接触微量金属的情况。已开发出量化六种化学物质的方法 人体尿液中砷的种类；这些物种包括砷甜菜碱，砷的一种无毒形式， 人们经常通过海鲜接触​​到一甲基砷酸，MMA(III)，一种剧毒物质 砷的化学形式。 TEA 核心还提供了在极低的浓度下对汞进行形态分析的方法。 水平和非常小的样品质量。我们的 GC-ICP-MS 方法可以检测不到 1 皮克 汞，并可以测定亚毫克样品重量中的汞种类。甲基汞是一种 汞的毒性极强，可通过食物链进行生物累积，因此必须能够 以确定非常低水平的汞形态。 TEA Core 继续突破低功耗的界限 水平微量元素检测，开发和验证微量元素的测定方法或 生物样品中的元素种类，目的是为达特茅斯 SRBP 研究人员提供 他们需要信息来对人类和生态系统健康做出更好的预测决策。