BETA CELL OSCILLATORY RESPONSE IS NOT RESULT OF A SINGLE COMPONENT ACTION

Beta 细胞振荡反应不是单一成分作用的结果

• 批准号: 7598513
• 负责人: Emma Heart
• 金额: $ 2.34万
• 依托单位: MARINE BIOLOGICAL LABORATORY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-12-01 至 2007-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7598513
• 关键词: Affect; Beta Cell; Cells; Computer Retrieval of Information on Scientific Projects Database; Environment; Funding; Glucose; Glycolysis; Grant; Hour; Institution; Insulin; Metabolism; Nutrient; Output; Pattern; Periodicity; Research; Research Personnel; Resources; Source; United States National Institutes of Health; deprivation; insulin secretion; methyl pyruvate; prevent; response

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Pulsatile insulin output, paralleled by oscillations in intracellular Ca2+, reflects oscillating metabolism within ¿-cell in response to secretory fuels. We questioned whether oscillatory periodicity is conserved or varied from stimulation to stimulation, whether glycolysis is essential for the manifestation of the oscillatory response and whether the environment of nutrient oversupply affects oscillatory regularity. We have determined that a ¿-cell oscillatory Ca2+ pattern is independent of the type of applied secretory fuel (glucose, methyl-pyruvate (MeP), ¿-ketoisocaproate (KIC). Single cells thus respond with the same pattern when repeatedly stimulated, regardless of the type of stimulatory fuel. Presence of sub-stimulatory (4 mM) glucose was not necessary to obtain oscillatory response to MeP or KIC. Several hours of glucose deprivation (cells were cultured in the presence of sub-stimulatory concentration of MeP), did not prevent fuel-stimulated oscillatory response, nor insulin secretion.

该副本是使用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这是调查员的机构。 脉冲胰岛素输出与细胞内Ca2+中的振荡平行，反映了``响应秘密燃料''内的振荡代谢。我们质疑振荡周期性是从刺激到刺激而变化的，糖酵解是否对于振荡反应的表现至关重要，以及营养过度供应的环境是否影响振荡的规律性。我们已经确定，一个细胞振荡Ca2+模式与应用的秘密燃料（葡萄糖，甲基 - 丙酮酸甲基甲基硫酸盐（MEP），€ - 酮二铜酸盐（KIC）（KIC）的类型无关。单个细胞反复响应相同的模式，当反复刺激时，无论是刺激的燃料的类型，无论是否刺激了（4 mm），无论是在刺激的燃料类型上，都不是kit kit kit。葡萄糖剥夺（在MEP的亚刺激浓度存在下培养细胞），并不能阻止燃料刺激的振荡反应，也不能阻止胰岛素分泌。