BETA CELL OSCILLATORY RESPONSE IS NOT RESULT OF A SINGLE COMPONENT ACTION

Beta 细胞振荡反应不是单一成分作用的结果

• 批准号: 7721107
• 负责人: Emma Heart
• 金额: $ 2.26万
• 依托单位: MARINE BIOLOGICAL LABORATORY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-12-01 至 2008-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7721107
• 关键词: Affect; Beta Cell; Cells; Computer Retrieval of Information on Scientific Projects Database; Environment; Funding; Glucose; Glycolysis; Grant; Hour; Institution; Insulin; Metabolism; Nutrient; Output; Pattern; Periodicity; Research; Research Personnel; Resources; Source; United States National Institutes of Health; deprivation; insulin secretion; methyl pyruvate; prevent; response

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Pulsatile insulin output, paralleled by oscillations in intracellular Ca2+, reflects oscillating metabolism within ¿-cell in response to secretory fuels. We questioned whether oscillatory periodicity is conserved or varied from stimulation to stimulation, whether glycolysis is essential for the manifestation of the oscillatory response and whether the environment of nutrient oversupply affects oscillatory regularity. We have determined that a ¿-cell oscillatory Ca2+ pattern is independent of the type of applied secretory fuel (glucose, methyl-pyruvate (MeP), ¿-ketoisocaproate (KIC). Single cells thus respond with the same pattern when repeatedly stimulated, regardless of the type of stimulatory fuel. Presence of sub-stimulatory (4 mM) glucose was not necessary to obtain oscillatory response to MeP or KIC. Several hours of glucose deprivation (cells were cultured in the presence of sub-stimulatory concentration of MeP), did not prevent fuel-stimulated oscillatory response, nor insulin secretion.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以出现在其他 CRISP 条目中 列出的机构是。 中心，不一定是研究者的机构。 脉动胰岛素输出，与细胞内 Ca2+ 的振荡平行，反映了 ¿ -细胞对分泌燃料的反应。我们质疑振荡周期性是否保守或随刺激而变化，糖酵解是否对于振荡反应的表现至关重要，以及营养过剩的环境是否影响振荡规律。 -细胞振荡 Ca2+ 模式与所用分泌燃料的类型无关（葡萄糖、丙酮酸甲酯 (MeP)、酮异己酸 (KIC)）。因此，无论刺激燃料的类型如何，单细胞在反复刺激时都会以相同的模式做出反应亚刺激 (4 mM) 葡萄糖的存在对于获得 MeP 或 KIC 几个小时的葡萄糖剥夺（细胞）的振荡反应不是必需的。在存在亚刺激浓度的 MeP 的情况下培养），不会阻止燃料刺激的振荡反应，也不会阻止胰岛素分泌。