Islet culture, shipping, and infusion device

胰岛培养、运输和输注装置

• 批准号: 7603106
• 负责人: John Wilson
• 金额: $ 118.42万
• 依托单位: WILSON WOLF MANUFACTURING CORPORATION
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-30 至 2011-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7603106
• 关键词: Address; Cell Culture Techniques; Characteristics; Communities; Devices; Diabetes Mellitus; Electronics; Gases; Grant; Health Care Costs; Incubators; Infusion procedures; Investigation; Islets of Langerhans; Marketing; Methods; Molecular Profiling; Outcome; Oxygen Consumption; Positioning Attribute; Preparation; Process; Protocols documentation; Quality of life; Relative (related person); Research; Role; Shipping; Ships; Societies; Techniques; Tissues; Transfusion; Transplantation; density; design; feeding; flasks; immunogenicity; islet; novel

DESCRIPTION (provided by applicant): The culture and shipment of islets are an integral part of islet transplant research. Flasks, petri dishes, and bags are the devices used to perform that role. However, these devices have inherent design characteristics that make them poorly suited to the unique needs of islet culture and islet shipping. This results in the use of far too many devices to maintain an islet preparation, a high potential for contamination, severely limited process control, and islet damage during shipping. Unfortunately, little effort has been undertaken to address the problems inherent to existing devices. We propose the creation of a superior device that is ideally suited to oxygenate islets and is tailored to the needs of the islet community. It will be the only device capable of culturing, shipping, and infusing an entire pancreatic islet isolation. This device is referred to as the single layer gas permeable device (SLD). Our investigation is expected to achieve five specific aims. Aim 1 will define a high-density SLD islet culture protocol that provides islets of superior quality and less immunogenicity than possible with existing devices when an entire islet preparation is cultured in a single device for at least seven-days without feeding. Outcomes will be determined by a variety of techniques including oxygen consumption rate (OCR), OCR/DNA, and molecular profiling. Aim 2 will adapt the SLD from a culture to a shipping device. Aim 3 will create a novel gyroscopic shipping container (GSC) that functions as an incubator without need of electronic controls and maintains the SLD in a standing position independent of how its outer container is oriented during shipping. This essentially allows islets to be cultured during shipping. Aim 4 will verify that the SLD of Aim 2 and the GSC of Aim 3 deliver islet tissue with superior viable islet yield, quality, and reduced immunogenicity relative to existing methods. Aim 5 will modify the SLD for islet transfusion and verify the superiority of the SLD for islet culture, shipping, and infusion relative to existing methods. Project Narrative: Islet transplants hold promise to provide a better quality of life for those afflicted with diabetes and reduce health care cost to society. There are no cell culture devices on the market that are designed with the attributes needed to efficiently oxygenate islets during culture and shipping. This grant is intended to create a gas permeable device (SLD) that can culture, ship, and infuse islets with maximum efficiency.

描述（由申请人提供）：胰岛的文化和运输是胰岛移植研究的组成部分。烧瓶，培养皿和袋子是用于执行该角色的设备。但是，这些设备具有固有的设计特征，使它们不适合胰岛文化和胰岛运输的独特需求。这导致使用太多的设备来维持胰岛准备，污染的可能性很高，过程控制严重有限以及运输过程中的胰岛损坏。不幸的是，几乎没有努力解决现有设备固有的问题。我们建议创建一种优秀的设备，该设备非常适合氧合胰岛，并根据胰岛社区的需求量身定制。它将是唯一能够培养，运输和注入整个胰岛隔离的设备。该设备称为单层气体可渗透设备（SLD）。我们的调查有望实现五个具体目标。 AIM 1将定义高密度的SLD Islet培养协议，该协议可提供优质质量且与现有设备的免疫原性相比，当整个胰岛制备在单个设备中培养至少七日而无需喂食时，现有设备的免疫原性。结果将由多种技术决定，包括氧消耗率（OCR），OCR/DNA和分子分析。 AIM 2将使SLD从文化转化为运输设备。 AIM 3将创建一个新型的陀螺式运输容器（GSC），该容器可作为孵化器起作用，而无需电子控制，并将SLD保持在站立位置，而与运输过程中其外部容器的定向无关。从本质上讲，这允许在运输过程中培养胰岛。 AIM 4将验证AIM 2的SLD和AIM 3的GSC传递胰岛组织具有出色的胰岛产量，质量和降低的免疫原性相对于现有方法。 AIM 5将修改胰岛输血的SLD，并验证SLD对胰岛培养，运输和输液相对于现有方法的优越性。 项目叙述：胰岛移植有望为患有糖尿病患者的人们提供更好的生活质量，并降低社会的医疗保健成本。市场上没有细胞培养设备的设计，其在培养和运输过程中有效地有效氧合胰岛所需的属性。该赠款旨在创建一种可渗透的设备（SLD），该设备可以以最高效率进行培养，运送和注入胰岛。