Islet culture, shipping, and infusion device

胰岛培养、运输和输注装置

• 批准号: 7114785
• 负责人: John Wilson
• 金额: $ 6.25万
• 依托单位: WILSON WOLF MANUFACTURING CORPORATION
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-30 至 2006-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7114785
• 关键词: animal tissue; biomedical equipment development; clinical research; consumable /disposable biomedical equipment; gas; growth media; human tissue; injection /infusion; insulin dependent diabetes mellitus; membrane permeability; miniature biomedical equipment; oxygen consumption; packaging material; pancreatic islet transplantation; pancreatic islets; tissue /cell culture

DESCRIPTION (provided by applicant): There exists a need for a single device that can culture, ship, and infuse islet. Flasks and petri dishes are the devices currently used to store islets. They are a weak link in the process because these devices have inherent design limitations that do not allow them to maintain islets at high viability during culture or shipping. The problem is the need to oxygenate islets by a gas/medium interface. The net result is that many devices are required for a single transplant, the process is not compatible with regulatory control, and precious islets are lost due to necrosis. There are no commercially available substitutes that offer a superior alternative. This grant is focuses upon creating a gas permeable device that cultures, ships, and infuses islets at high viability in a manner compatible with regulatory control. This device will be superior to any commercially available alternatives. By eliminating the link between oxygen delivery and a gas/medium interface, a device that allows high density culture at high viability becomes available. The islets are at improved oxygen tension, with extra medium capacity, from culture through shipping. Primary and backup device configurations are proposed. One configuration is based on culturing islets at high density in an extremely easy to use device. A backup device integrates a high amount of surface area to house islets at a wide range of possible densities. Aim 1 determines which configuration will be pursued, and creates its design specifications. Of primary interest is the optimal gas permeable surface area to medium volume ratios that indicate device size and shape. Aim 2 produces small scale prototypes of the device and quantifies the performance relative to the flask using porcine and human islets, and provides design specifications for Aim 3. Aim 3 produces full scale device prototypes and quantifies the performance relative to the flask using porcine and human islets. If the novel device proves superior to the flask, specifications of the device will be delivered to Phase II which will investigate making the device suitable for shipping and infusing islets.

描述（由申请人提供）： 存在一个可以培养，运送和注入胰岛的单个设备。烧瓶和培养皿是目前用于存储胰岛的设备。它们在此过程中是一个薄弱的联系，因为这些设备具有固有的设计局限性，这些局限性不允许它们在文化或运输过程中保持胰岛的高活力。问题是需要通过气/中型界面氧合胰岛。最终结果是，单个移植需要许多设备，该过程与调节控制不兼容，并且由于坏死而丢失了珍贵的胰岛。没有可提供优质替代品的市售替代品。 该赠款的重点是创建一种可渗透的装置，该设备以与调节控制的方式相容的方式培养，发射和注射胰岛。该设备将优于任何可商购的替代方案。通过消除氧气递送与气体/媒介界面之间的联系，可以使用高密度培养的设备可用。从培养到运输，胰岛处于改善的氧气张力，具有额外容量的氧气张力。提出了主和备份设备配置。一种配置是基于非常易于使用的设备的高密度培养胰岛。备用设备将大量的表面积集成到在广泛可能的密度范围内的房屋胰岛。 AIM 1确定将追求哪种配置，并创建其设计规格。主要感兴趣的是指示设备尺寸和形状的最佳气体可渗透表面积与中等体积比。 AIM 2生产小规模的设备原型，并使用猪和人类胰岛量化相对于烧瓶的性能，并为AIM 3提供设计规范。AIM3生成了完整的设备原型，并使用猪和人类胰岛量化了相对于烧瓶的性能。如果新型设备证明优于烧瓶，则将将设备的规格传递到II期，这将调查该设备适合运输和注入胰岛。

项目成果

Assessment of tissue-engineered islet graft viability by fluorine magnetic resonance spectroscopy.

通过氟磁共振波谱评估组织工程胰岛移植物的活力。

• DOI: 10.1016/j.transproceed.2011.09.009
• 发表时间: 2011
• 期刊: Transplantation proceedings
• 影响因子: 0.9
• 作者: Suszynski,TM;Avgoustiniatos,ES;Stein,SA;Falde,EJ;Hammer,BE;Papas,KK
• 通讯作者: Papas,KK

Islet preparation purity is overestimated, and less pure fractions have lower post-culture viability before clinical allotransplantation.

• DOI: 10.1016/j.transproceed.2014.06.011
• 发表时间: 2014-07
• 期刊: Transplantation proceedings
• 影响因子: 0.9
• 作者: Kitzmann JP;Karatzas T;Mueller KR;Avgoustiniatos ES;Gruessner AC;Balamurugan AN;Bellin MD;Hering BJ;Papas KK
• 通讯作者: Papas KK

Metabolic profile of pancreatic acinar and islet tissue in culture.

培养物中胰腺腺泡和胰岛组织的代谢特征。

• DOI: 10.1016/j.transproceed.2014.06.003
• 发表时间: 2014
• 期刊: Transplantation proceedings
• 影响因子: 0.9
• 作者: Suszynski,TM;Mueller,KR;Gruessner,AC;Papas,KK
• 通讯作者: Papas,KK

Manufacturing porcine islets: culture at 22ýýýýC has no advantage above culture at 37ýýýýC: a gene expression evaluation.

制造猪胰岛：22°C 培养比 37°C 培养没有优势：基因表达评估。

• DOI: 10.1111/xen.12048
• 发表时间: 2013
• 期刊: Xenotransplantation
• 影响因子: 3.9
• 作者: Mueller,KateR;Martins,KyraV;Murtaugh,MichaelP;Schuurman,Henk-Jan;Papas,KlearchosK
• 通讯作者: Papas,KlearchosK