DIETARY PROTEIN AND ENDOGENOUS OXALATE SYNTHESIS

膳食蛋白质和内源性草酸盐合成

• 批准号: 7607704
• 负责人: ROSS P HOLMES
• 金额: $ 6.89万
• 依托单位: WAKE FOREST UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-03-01 至 2008-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7607704
• 关键词: Amino Acids; Calcium Oxalate; Computer Retrieval of Information on Scientific Projects Database; Diet; Dietary Proteins; Excretory function; Female; Funding; Grant; Hepatocyte; Hereditary Disease; Human; Institution; Intake; Kidney Calculi; Laboratories; Liver; Macronutrients Nutrition; Metabolic Pathway; Metabolism; Micronutrients; Organ; Oxalates; Play; Production; Proteins; Research; Research Personnel; Resources; Role; Source; Testing; United States National Institutes of Health; dietary constituent; dietary control; male; research study; urinary

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The amount of oxalate synthesized in the body is a critical factor in the primary hyperoxalurias, which are rare genetic diseases, and also plays an important role in calcium oxalate kidney stone formation. The metabolic pathways and dietary constituents that contribute to this endogenous synthesis of oxalate have not been clearly defined. The liver is the major organ producing endogenous oxalate and experiments in our laboratory with cultured hepatocytes indicate that amino acid metabolism is a primary source of this oxalate. We hypothesize that amino acid metabolism is an important contributor to endogenous oxalate production in humans and that this metabolism will be enhanced on high protein diets. A previous study we conducted suggested that females, but not males, responded to an increased protein intake with an increased endogenous synthesis of oxalate. The interpretation of this study was confounded, however, by a lack of control of dietary oxalate. To test our hypothesis that amino acid metabolism is a significant contributor to endogenous oxalate synthesis in both males and females, we will examine the urinary excretion of oxalate in subjects consuming diets of varying protein content. Diets will be controlled in macro- and micronutrients, including oxalate, to limit the effects of dietary oxalate on urinary oxalate excretion.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 体内合成的草酸盐量是原发性高氧化盐的关键因素，这是罕见的遗传疾病，并且在草酸钙肾结石的形成中也起着重要作用。 尚未明确定义有助于草酸内源性合成的代谢途径和饮食成分。 肝脏是产生内源性草酸盐的主要器官，并在实验室中使用培养的肝细胞进行实验，表明氨基酸代谢是该草酸盐的主要来源。 我们假设氨基酸代谢是人类内源性草酸盐产生的重要因素，并且这种代谢将在高蛋白质饮食上得到增强。我们先前进行的一项研究表明，女性（而不是男性）对蛋白质摄入量的增加反应，并且内源性草酸盐的内源性合成增加。然而，由于缺乏对草酸盐饮食的控制，这项研究的解释被混淆了。为了检验我们的假设，即氨基酸代谢是男性和女性内源性草酸盐合成的重要原因，我们将检查消耗蛋白质含量饮食的患者中草酸盐的尿液排泄。饮食将在包括草酸盐在内的宏观和微量营养素中受到控制，以限制草酸饮食中草酸盐对草酸尿液排泄的影响。