The Werner Syndrome Protein in DNA Replication, Mutagenesis and Genomic Stability

DNA 复制、诱变和基因组稳定性中的维尔纳综合征蛋白

基本信息

批准号：
7728844
负责人：
LAWRENCE A LOEB
金额：
$ 25.66万
依托单位：
UNIVERSITY OF WASHINGTON
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-09-30 至 2011-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7728844
关键词：
Adolescence Age Age of Onset Alleles Amino Acid Substitution Arthritis Biochemical Biological Assay Biotin Blood Brazil C57BL/6 Mouse Cataract Cell Cycle Cell Cycle Completion Cell Cycle Kinetics Cell Line Cell physiology Cells Chromosomal Rearrangement Chromosome abnormality Clinical DNA DNA Damage DNA Structure DNA amplification DNA biosynthesis DNA lesion DNA-Directed DNA Polymerase Defect Deletion Mutation Diabetes Mellitus Digestion Disease Eukaryotic Cell Exhibits Exonuclease Experimental Designs Family member Fibroblasts Foundations Frequencies Future Genes Genetic Polymorphism Genome Genome Stability Genomic Instability Genomics Grant Hair Heart Diseases Heterozygote Human Human Genome Inborn Genetic Diseases Incidence Individual Induced Mutation Insecta Introns Knowledge Laboratories Lesion Malignant Neoplasms Measures Medical Medicine Methods Mexico Mus Mutagenesis Mutate Mutation Native-Born Neomycin Nucleotides Pathology Pathway interactions Persons Phase Phenotype Phosphodiesterase I Play Point Mutation Population Positioning Attribute Premature aging syndrome Prevalence Procedures Process Property Protein Biosynthesis Proteins Public Health Reading Recombinants Research Resistance Resources Role Sampling Screening procedure Single Nucleotide Polymorphism Site Southern Blotting Streptavidin Stretching Structure Substrate Specificity TP53 gene Time Tissues United States Variant Werner Syndrome Work age related cell type embryonic stem cell exodeoxyribonuclease fiber cell gel electrophoresis genetic pedigree helicase human DNA human WRN protein human disease insight lymphoblast mRNA Decay magnetic beads mutant preference proband prolylleucine research study restriction enzyme synthetic construct vector

项目摘要

The proposed experiments will establish a foundation for future studies on the relationship of WRN polymorphisms to age-associated human diseases. These fundamental studies will hasten the pace of research on the relationship of mutations and age-associated diseases and thus are central to medicine and public health. All of the procedures have been established and are ongoing in our laboratory and can be completed within two years.

拟议的实验将为未来研究建立基础 WRN多态性与与年龄相关的人类疾病的关系。这些基本研究将加快有关突变与年龄相关疾病关系的研究步伐，因此是医学和公共卫生的核心。所有程序均已建立并正在我们的实验室中进行，可以在两年内完成。