Investigations of the Bioinorganic Chemistry of Iron: New Siderophores

铁的生物无机化学研究：新铁载体

基本信息

批准号：
7531799
负责人：
Alison Butler
金额：
$ 24.54万
依托单位：
UNIVERSITY OF CALIFORNIA SANTA BARBARA
依托单位国家：
美国
项目类别：
财政年份：
1988
资助国家：
美国
起止时间：
1988-02-01 至 2011-11-30
项目状态：
已结题

项目摘要

The objectives of this research are to improve our understanding of the mechanisms of metal acquisition by microorganisms in the marine environment. Interest in the mechanisms of acquisition of iron (and other metal ions) by oceanic bacteria derives from the unique transition metal ion composition of the ocean. Iron is a limiting nutrient to marine microorganisms over much of the world's oceans and has now been shown to regulate the global carbon cycle. Results from the last grant period demonstrated that the majority of marine siderophore structures fall within two categories: 1) suites of self-assembling amphiphilic peptide siderophores including the marinobactins, aquachelins, amphibactins, ochrobactins and synechobactins, which are produced by phylogenetically distinct genera of bacteria; and 2) siderophores containing an alpha- hydroxycarboxylic acid moiety (e.g., beta-hydroxyaspartic acid, citric acid) which when coordinated to Fe(lll) are photoreactive, including the ferric complexes of aquachelins, marinobactins, aerobactin, petrobactins, synechobactins and ochrobactins. The hypothesis to be tested is that the amphiphilic character and photoreactivity confer an advantage for microbial growth tailored to the chemical and physical constraints of the ocean. Certain siderophores produced by pathogenic bacteria bear a structural resemblance to the amphiphilic siderophores produced by marine bacteria, including the mycobactins and carboxymycobactins, produced by mycobacteria, such as M. tuberculosis, which caused tuberculosis and acinetoferrin produced by Acinetobacter haemolyticus which causes respiratory disease. Little is known, however, about the importance of the amphiphilic and photoreactive properties of the mycobacterial and acinetobacter siderophores on the disease state. The specific aims of the proposed research are to i) investigate the photoreactivity of the marine ferric siderophores with alpha-hydroxy acid groups, ii) investigate the amphiphilic properties of the suites of marine amphiphilic siderophores, iii)investigate the photoreactivity and amphiphilic effects on iron acquisition by the source bacteria, and iv) isolate and characterize new siderophores produced by selected other marine bacteria.

这项研究的目标是提高我们对金属采集机制的理解通过海洋环境中的微生物。对铁的获取机制的兴趣（以及其他金属离子）由海洋细菌源自海洋独特的过渡金属离子组成。铁是限制了世界上许多海洋海洋微生物的养分，现在已被证明调节全球碳循环。上一个赠款期的结果表明大多数海洋铁载体结构属于两个类别：1）自组装的两亲肽的套件铁载体，包括海洋生丁物，aquachelins，苯丙胺，绿色乳酸素和synechobactins，synechobactins，这是由细菌的系统发育不同的属产生的； 2）含有α-的铁载体羟基羧酸部分（例如，β-羟基大规模，柠檬酸），当协调为Fe（LLL）时具有光反应性，包括Aquachelins，Marinobactins，Aerobactin，Petrobactins，合成蛋白和甲状腺素。要检验的假设是两亲性和光电反应赋予了针对化学和物理限制的微生物生长的优势海洋。致病细菌产生的某些铁载体与海洋细菌产生的两亲性铁载体，包括霉菌素和羧甲状腺素，由分枝杆菌产生的，例如结核分枝杆菌，引起结核病和阿甲铁铁蛋白通过引起呼吸系统疾病的溶血杆菌溶血性。但是，关于分枝杆菌和肌分杆的两亲性和光反应性特性的重要性疾病状态的铁载体。拟议研究的具体目的是i）研究海洋铁的光反应性 α-羟基酸基团的铁载体，ii）研究海洋套房的两亲性特性两亲性铁载体，iii）研究对铁对铁采集的光反应性和两亲性影响源细菌和iv）分离并表征选择其他海洋生产的新的铁载体细菌。