Investigations of the Bioinorganic Chemistry of Iron: New Siderophores

铁的生物无机化学研究：新铁载体

基本信息

批准号：
7155539
负责人：
Alison Butler
金额：
$ 24.74万
依托单位：
UNIVERSITY OF CALIFORNIA SANTA BARBARA
依托单位国家：
美国
项目类别：
财政年份：
1988
资助国家：
美国
起止时间：
1988-02-01 至 2009-11-30
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): The objectives of this research are to improve our understanding of the mechanisms of metal acquisition by microorganisms in the marine environment. Interest in the mechanisms of acquisition of iron (and other metal ions) by oceanic bacteria derives from the unique transition metal ion composition of the ocean. Iron is a limiting nutrient to marine microorganisms over much of the world's oceans and has now been shown to regulate the global carbon cycle. Results from the last grant period demonstrated that the majority of marine siderophore structures fall within two categories: 1) suites of self-assembling amphiphilic peptide siderophores including the marinobactins, aquachelins, amphibactins, ochrobactins and synechobactins, which are produced by phylogenetically distinct genera of bacteria; and 2) siderophores containing an alpha- hydroxycarboxylic acid moiety (e.g., beta-hydroxyaspartic acid, citric acid) which when coordinated to Fe(lll) are photoreactive, including the ferric complexes of aquachelins, marinobactins, aerobactin, petrobactins, synechobactins and ochrobactins. The hypothesis to be tested is that the amphiphilic character and photoreactivity confer an advantage for microbial growth tailored to the chemical and physical constraints of the ocean. Certain siderophores produced by pathogenic bacteria bear a structural resemblance to the amphiphilic siderophores produced by marine bacteria, including the mycobactins and carboxymycobactins, produced by mycobacteria, such as M. tuberculosis, which caused tuberculosis and acinetoferrin produced by Acinetobacter haemolyticus which causes respiratory disease. Little is known, however, about the importance of the amphiphilic and photoreactive properties of the mycobacterial and acinetobacter siderophores on the disease state. The specific aims of the proposed research are to i) investigate the photoreactivity of the marine ferric siderophores with alpha-hydroxy acid groups, ii) investigate the amphiphilic properties of the suites of marine amphiphilic siderophores, iii) investigate the photoreactivity and amphiphilic effects on iron acquisition by the source bacteria, and iv) isolate and characterize new siderophores produced by selected other marine bacteria.

描述（由申请人提供）：这项研究的目的是提高我们对海洋环境中微生物获取金属获取机制的理解。海洋细菌对获得铁（和其他金属离子）的获取机制的兴趣来自海洋独特的过渡金属离子组成。铁是世界上大部分海洋中海洋微生物的一种限制，现在已被证明可以调节全球碳循环。上一个赠款期间的结果表明，大多数海洋铁载体结构属于两类：1）自组成的两亲性肽铁载体的套件在内and 2) siderophores containing an alpha- hydroxycarboxylic acid moiety (e.g., beta-hydroxyaspartic acid, citric acid) which when coordinated to Fe(lll) are photoreactive, including the ferric complexes of aquachelins, marinobactins, aerobactin, petrobactins, synechobactins and ochrobactins.要检验的假设是，两亲性特征和光电反应性赋予了针对海洋化学和物理限制的微生物生长的优势。致病细菌产生的某些辅助粒子与海洋细菌所产生的两亲性铁载体具有结构相似之处，包括结核菌和结核菌的结核细菌产生，例如结核分枝杆菌产生，例如导致结核病和刺激性的呼吸症。然而，鲜为人知的是，分枝杆菌和杆菌杆菌对疾病状态的两亲性和光反应性特性的重要性。拟议的研究的具体目的是i）研究海洋铁铁铁载体与α-羟基酸基团的光反应性，ii）研究海洋两亲性载体的套件的套头族特性，iii iii）研究了对光的鉴定和分支机构对铁的疾病及其对铁的疾病的影响，及其分支机构的影响，以及分支机构的特征，并进行了分支机构，并进行了分支机构的影响，并进行了分支机构的影响，及其分支机构的影响及其分支机构及其分支机构及其分支机构及其对铁的效果由选定的其他海洋细菌产生。