FUNCTION OF MACAQUE SPERM PROTEINS IN FERTILIZATION

猕猴精子蛋白在受精过程中的功能

• 批准号: 7562136
• 负责人: PAUL PRIMAKOFF
• 金额: $ 5.74万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-05-01 至 2008-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7562136
• 关键词: Binding; Cells; Charge; Computer Retrieval of Information on Scientific Projects Database; Contraceptive methods; Female; Fertilization; Funding; Grant; Human; Immune; Infertility; Institution; Ligands; Liquid substance; Macaca; Masks; Membrane Proteins; Oligosaccharides; Play; Reporting; Reproduction; Research; Research Personnel; Resources; Role; Sialic Acids; Source; Surface; United States National Institutes of Health; Zona Pellucida; insight; interest; nonhuman primate; reproductive; sperm cell; sperm protein; stem

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Objective: Our lab has had a long standing interest in identifying and characterizing sperm surface proteins that play a role in the interaction of sperm with the fluids and cells of the female reproductive tract. Our findings in non-human primates are relevant to issues in human reproduction, providing insights into causes of infertility as well as identifying possible targets for contraception. We previously reported that DEFB126 (formerly ESP13.2) coats the entire surface of macaque sperm and remains until sperm become capacitated. The release of DEFB126 from sperm during capacitation is required for sperm recognition and binding of the zona pellucida, suggesting that DEFB126 masks zona pellucida ligands on the sperm surface. Furthermore, DEFB126 appears to masks all sperm surface components and protects sperm from immune recognition. Immunoprotection appears to stem from the highly glycosylated carboxyl domain which is comprised of oligosaccharides that terminate in sialic acid. DEFB126 provides uniform negative charge over the surface of sperm that is significantly reduced as sperm release DEFB126 during capacitation.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 目的：我们的实验室长期以来一直致力于鉴定和表征精子表面蛋白，这些蛋白在精子与女性生殖道液体和细胞的相互作用中发挥作用。我们在非人类灵长类动物中的发现与人类生殖问题相关，提供了对不孕原因的见解以及确定可能的避孕目标。我们之前报道过 DEFB126（以前称为 ESP13.2）覆盖猕猴精子的整个表面，并一直保留到精子获能为止。 获能过程中精子释放 DEFB126 是精子识别和结合透明带所必需的，这表明 DEFB126 掩盖了精子表面的透明带配体。 此外，DEFB126 似乎可以掩盖所有精子表面成分并保护精子免受免疫识别。 免疫保护似乎源于高度糖基化的羧基结构域，该结构域由末端为唾液酸的寡糖组成。 DEFB126 在精子表面提供均匀的负电荷，随着精子在获能期间释放 DEFB126，该负电荷显着减少。