FUNCTION OF MACAQUE SPERM PROTEINS IN FERTILIZATION

猕猴精子蛋白在受精过程中的功能

• 批准号: 7562136
• 负责人: PAUL PRIMAKOFF
• 金额: $ 5.74万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-05-01 至 2008-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7562136
• 关键词: Binding; Cells; Charge; Computer Retrieval of Information on Scientific Projects Database; Contraceptive methods; Female; Fertilization; Funding; Grant; Human; Immune; Infertility; Institution; Ligands; Liquid substance; Macaca; Masks; Membrane Proteins; Oligosaccharides; Play; Reporting; Reproduction; Research; Research Personnel; Resources; Role; Sialic Acids; Source; Surface; United States National Institutes of Health; Zona Pellucida; insight; interest; nonhuman primate; reproductive; sperm cell; sperm protein; stem

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Objective: Our lab has had a long standing interest in identifying and characterizing sperm surface proteins that play a role in the interaction of sperm with the fluids and cells of the female reproductive tract. Our findings in non-human primates are relevant to issues in human reproduction, providing insights into causes of infertility as well as identifying possible targets for contraception. We previously reported that DEFB126 (formerly ESP13.2) coats the entire surface of macaque sperm and remains until sperm become capacitated. The release of DEFB126 from sperm during capacitation is required for sperm recognition and binding of the zona pellucida, suggesting that DEFB126 masks zona pellucida ligands on the sperm surface. Furthermore, DEFB126 appears to masks all sperm surface components and protects sperm from immune recognition. Immunoprotection appears to stem from the highly glycosylated carboxyl domain which is comprised of oligosaccharides that terminate in sialic acid. DEFB126 provides uniform negative charge over the surface of sperm that is significantly reduced as sperm release DEFB126 during capacitation.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 目的：我们的实验室对识别和表征精子表面蛋白的兴趣很长，这些蛋白在精子与女性生殖道的液体和细胞的相互作用中起作用。我们在非人类灵长类动物中的发现与人类繁殖的问题有关，提供了对不育的原因的见解，并确定了可能的避孕目标。我们先前报道说，DEFB126（以前是ESP13.2）覆盖了猕猴精子的整个表面，并保留到精子被电容为止。 对于精子识别和结合了Zona pellucida，需要从精子中释放DEFB126，这表明在精子表面上掩盖了Zona pellucida配体。 此外，DEFB126似乎掩盖了所有精子表面成分，并保护精子免于免疫识别。 免疫保护似乎源于高度糖基化的羧基结构域，该羧基结构域由终止在唾液酸中终止的寡糖组成。 DEFB126在精子表面上提供了均匀的负电荷，这在电容过程中被显着降低，因为精子释放了126。