ACTIVITY #1 - PROTEOMICS DISCOVERY FACILITY

活动

• 批准号: 7715296
• 负责人: LOYDA M MELENDEZ
• 金额: $ 39.81万
• 依托单位: UNIVERSITY OF PUERTO RICO MED SCIENCES
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-08-01 至 2009-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7715296
• 关键词: Affect; Area; Arts; Bioinformatics; Biological; Biological Markers; Biomedical Research; Cardiovascular Diseases; Clinical; Collaborations; Communicable Diseases; Complement; Computer Retrieval of Information on Scientific Projects Database; Core Facility; Diabetes Mellitus; Disease; Disease Progression; Equipment; Faculty; Fostering; Funding; Genetic; Goals; Grant; HIV; High Pressure Liquid Chromatography; Institution; Laboratories; Malignant Neoplasms; Medical; Medical center; Minority; Nebraska; Pilot Projects; Population; Productivity; Proteins; Proteomics; Puerto Rico; Research; Research Infrastructure; Research Personnel; Research Project Grants; Resources; Science; Services; Source; Students; System; Technical Expertise; Technology; Training; United States National Institutes of Health; Universities; Woman; fast protein liquid chromatography; health disparity; instrument; statistics

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Proteomics is an emerging area of research that has been applied successfully to identify cellular biomarkers of disease progression. Ciphergen's ProteinChip¿ technology is an important component of the proteomics platform for the rapid discovery of multiple biomarkers when used with HPLC, FPLC and LC/MS/MS for identification of proteins. Statistics/Bioinformatics is also necessary to complement the proteomics platform that has been established at University of Puerto Rico Medical Sciences Campus (UPR MSC) in collaboration with experts at University of Nebraska Medical Center (UNMC). The long term goal of this Core Facility is to enhance our institutional biomedical research capability by establishing the necessary platform and expertise in proteomics to basic and clinical projects related to biomarker discovery. The following specific aims are proposed: 1. To provide the infrastructure necessary to characterize, and isolate differentially expressed proteins in populations with health disparities. 2. To increase the capabilities of performing research projects in proteomics among faculty and graduate students at the UPR-MSC. 3.To maintain a state of the art proteomic Core laboratory available for faculty and students and provide advice, service and training of proteomics analyses using diverse approaches in accordance to each biological system. 4.To prepare the institution for protein identification and analysis by fostering collaboration of the Biostatisticians in the mainland. This research infrastructure will be used by the UPR-MSC investigators who have started using the SELDI-TOF in pilot projects that affect our population in the areas of HIV Disease, Cancer, Diabetes, Cardiovascular diseases, Genetics and Infectious diseases. Since Protein Discovery requires sophisticated equipments, we propose to develop a Proteomics Core Facility with the minimum necessary instruments and technical expertise to provide access to investigators at UPR-MSC and increase their productivity. The relevance of this Activity is the reduction in Health Disparities by enhancing our biomedical research capability in the diseases that affect minority populations including women.

该副本是使用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这是调查员的机构。 蛋白质组学是一个新兴领域，已成功应用于鉴定疾病进展的细胞生物标志物。 Ciphergen的ProteinChip¿技术是蛋白质组学平台的重要组成部分，用于与HPLC，FPLC和LC/​​MS/MS一起快速发现多个生物标志物，以鉴定蛋白质。统计/生物信息学对于完成波多黎各大学医学科学校园（UPR MSC）建立的蛋白质组学平台也是必要的，与内布拉斯加州大学医学中心（UNMC）的专家合作。该核心设施的长期目标是通过建立与生物标志物发现相关的基本和临床项目的蛋白质组学的必要平台和专业知识来增强我们的机构生物医学研究能力。提出了以下具体目标：1。提供表征表征和分离出健康差异种群中不同表达蛋白质所需的基础设施。 2。为了增加UPR-MSC的教师和研究生中蛋白质组学研究项目的能力。 3.维持可用于教职员工和学生的最先进的蛋白质组学核心实验室，并根据每个生物系统使用潜水员的方法提供蛋白质组学分析的建议，服务和培训。 4.通过促进大陆生物统计学家的合作，为蛋白质识别和分析做好准备。 UPR-MSC研究人员将使用这项研究基础设施，他们已经开始在艾滋病毒，癌症，癌症，糖尿病，心血管疾病，遗传学和感染性疾病的领域中使用SELDI-TOF。由于蛋白质发现需要复杂的设备，因此我们建议开发一种具有最低必要工具和技术专长的蛋白质组学核心设施，以便在UPR-MSC访问研究人员并提高其生产率。这项活动的相关性是通过增强影响包括妇女在内的少数群体的疾病中的生物医学研究能力来减少健康差异。