Androgens and molecular mediators of motoneuron repair

雄激素和运动神经元修复的分子介质

基本信息

批准号：
7409071
负责人：
KEITH N FARGO
金额：
$ 4.33万
依托单位：
LOYOLA UNIVERSITY CHICAGO
依托单位国家：
美国
项目类别：
财政年份：
2006
资助国家：
美国
起止时间：
2006-05-15 至 2009-03-28
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7409071
关键词：
Adult Androgens Animals Axotomy B-Lymphocytes CD4 Positive T Lymphocytes Cell Death Cell Survival Cessation of life Control Animal Crush Injury Diagnosis Disease Electron Microscopy Exposure to Face Facial nerve structure FarGo Fellowship Future Genes Goals Hamsters Heat-Shock Response Immune system In Situ Hybridization In Vitro Individual Injury Knockout Mice Lead Light Measures Mediating Mediator of activation protein Medical Messenger RNA Methods Molecular Motor Neurons Mus Names Nerve Crush Neuraxis Neurodegenerative Disorders Neurons Neurosciences Peripheral Peripheral nerve injury Procedures Proteins Readability Research Research Design Research Training Shapes Spinal Spinal cord injury Techniques Testosterone Text Thinking Tissues Transgenic Mice Traumatic Nerve Injury Up-Regulation Work immunocytochemistry in vivo injured interest male neuronal survival novel pituitary adenylate cyclase activating polypeptide prevent protein expression recombinase reconstitution repaired research study response to injury steroid hormone

项目摘要

DESCRIPTION (provided by applicant): Motoneuron injury and degeneration are important medical issues, with thousands of people diagnosed with spinal motoneuron diseases, spinal cord injuries, and peripheral nerve injuries every year. The long-term goals of this proposal are to contribute to our understanding of the motoneuron's cellular and molecular response to injury. Using a variety of molecular neuroscience techniques, I will study the relationship of steroid hormones to a novel protein (neuritin) thought to be involved in the ability of neurons to change their overall shape, and therefore their functional connectivity. Additionally, I will examine the relationship between steroid hormones and the immune system in promoting neuronal survival in the central nervous system following axonal injury. Techniques to be used include in situ hybridization, immunocytochemistry, electron microscopy, and the use of transgenic mice. This research could potentially lead to future treatment strategies for traumatic nerve injuries and neurodegenerative diseases.

描述（申请人提供）：运动神经元损伤和变性是重要的医学问题，每年有数千人被诊断患有脊髓运动神经元疾病、脊髓损伤和周围神经损伤。该提案的长期目标是有助于我们了解运动神经元对损伤的细胞和分子反应。我将使用各种分子神经科学技术，研究类固醇激素与一种新型蛋白质（神经突蛋白）的关系，该蛋白质被认为与神经元改变其整体形状的能力有关，从而影响其功能连接。此外，我将研究类固醇激素与免疫系统在促进轴突损伤后中枢神经系统神经元存活方面的关系。所使用的技术包括原位杂交、免疫细胞化学、电子显微镜和转基因小鼠的使用。这项研究可能会导致未来创伤性神经损伤和神经退行性疾病的治疗策略。