Multi-spectral Mosaic (MSM) Digital Imaging Sensor for Microscopy Applications

适用于显微镜应用的多光谱马赛克 (MSM) 数字成像传感器

• 批准号: 7407933
• 负责人: Steve Michael Savoy
• 金额: $ 25.14万
• 依托单位: NANOHMICS, INC.
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-07-21 至 2010-07-20
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7407933
• 关键词: Algorithms; Binding; Biomedical Engineering; Collaborations; Color; Cost Effectiveness Analysis; Cypress; Data; Deposition; Development; Devices; Diagnostic; Digital Photography; Dyes; Elements; Fluorescent in Situ Hybridization; Gray unit of radiation dose; Image; Immobilization; In Situ Hybridization; Karyotype determination procedure; Lead; Libraries; Light; Liquid substance; Methods; Microarray Analysis; Microfluidics; Microscopy; Microspheres; Numbers; Oligonucleotide Microarrays; Oligonucleotides; Optics; Pattern; Phase; Polymers; Preparation; Process; Range; Reproduction; Research; Resolution; Semiconductors; Speed; Standards of Weights and Measures; Streptavidin; Surface; System; Techniques; Technology; Testing; Tissues; Ultrafine; computerized data processing; concept; cost; density; design; digital imaging; instrument; instrumentation; next generation; novel; particle; seal; sensor

DESCRIPTION (provided by applicant): Compact, low-cost digital imaging systems that combine the benefits of high spatial and spectral resolution are in demand for bioanalytical analyses such as in situ hybridization (ISH) and fluorescence in situ hybridization (FISH) karyotyping, tissue and cellular diagnostics, and microarrays. Advances in commercial image sensors have led to pixel densities which provide exquisite spatial resolution. Despite pixel densities sufficient to resolve ultrafine features with large fields of view, commercially available color CMOS cameras are still limited in the level of spectroscopic (chromaticity) resolution. Color imaging systems are generally restricted by the Bayer color filter array (CFA) mosaic patterning (i.e. usually RGB three color filter arrays (CFAs). These color camera chips are designed to meet minimal color reproduction requirements for digital photography. Higher spectroscopic band definition is achieved through combination of a monochrome image sensor with dispersive/filter elements that are bulky and expensive, particularly for those involving motorized switching between filter sets. Likewise, electronically tunable filters (e.g. LCD, acousto-optic, Sagnac, etc.) that slow image acquisition speed are not ideal. Elimination of external dispersive elements and slow tunable filters requires directly integrating higher spectral definition into the mosaic pattern on the image sensor (CMOS/CCD). Nanohmics, in collaboration with Atactic Technologies, propose to develop a process for expanding the spectral resolution and range (e.g. UV, visible and near-IR) of Color Filter Arrays (CFAs) directly on the CMOS sensor surface. Furthermore, these Multi-Spectral Mosaic (MSM) patterns will be deposited using a novel method that utilizes the lock-and- key registration of oligonucleotide micorarrays to direct surface immobilization of core- dyed microspheres into the mosaic pattern on the image sensor. The proposed method is vastly different than standard serial lithographic processing currently used in commercial preparation of existing color camera CFAs. The proposed oligo-directed immobilization will lead to the development of the entire mosaic pattern in a single processing step. This proposed technique was exploit biomolecular recognition to bioengineer the material layer in a commercial device application.Project Narrative The expansion of spectral capabilities of a compact image sensor would enable more rapid and cost effective analyses of biomolecular processes in a number of fields including microarray analysis, diagnostics, and karyotyping. Furthermore, enhanced spectral resolution will lead to higher color fidelity reproduction for all image sensor applications. The use of biomolecular materials for bioengineering is an important enabled feature of the proposed research

描述（由申请人提供）：结合高空间和光谱分辨率的好处的紧凑，低成本的数字成像系统，需要进行生物分析分析，例如原位杂交（ISH）和荧光原位杂交（FISH）KARYOTYPING（FISH）KARYOTYPING，组织和细胞诊断，诊断和微型货物。商业图像传感器的进步导致像素密度可提供精美的空间分辨率。尽管像素密度足以解析具有较大视野的超细特征，但在光谱（色度）分辨率的水平上，市售颜色CMOS摄像机仍受到限制。 Color imaging systems are generally restricted by the Bayer color filter array (CFA) mosaic patterning (i.e. usually RGB three color filter arrays (CFAs). These color camera chips are designed to meet minimal color reproduction requirements for digital photography. Higher spectroscopic band definition is achieved through combination of a monochrome image sensor with dispersive/filter elements that are bulky and expensive, particularly for those involving motorized switching between filter同样，可调的过滤器（例如LCD，声学，Sagnac等），慢速的图像采集速度并不理想。直接在CMOS传感器表面上的彩色滤波器阵列（CFA）的光谱分辨率和范围（例如UV，可见和近IR）。此外，这些多光谱马赛克（MSM）模式将使用一种新的方法沉积，该方法利用寡核苷酸示威的锁定和钥匙注册将核心染色的微分球固定到图像传感器上的镶嵌图中。所提出的方法与当前用于现有彩色摄像头CFA的商业准备中使用的标准串行光刻处理大不相同。提出的寡寡固定将在单个处理步骤中导致整个马赛克模式的发展。这项提出的技术是利用生物分子识别的，以生物工程在商业设备应用中的材料层。项目叙述 紧凑图像传感器的光谱功能的扩展将使对许多领域的生物分子过程进行更快，更具成本效益的分析，包括微阵列分析，诊断和核分型。此外，增强的光谱分辨率将导致所有图像传感器应用的颜色更高。使用生物分子材料进行生物工程是拟议研究的重要功能