Dynamic regulation of neuroinflammation and IL-1b-trap

神经炎症和 IL-1b-trap 的动态调节

基本信息

批准号：
10726269
负责人：
Ning Quan
金额：
$ 41.94万
依托单位：
FLORIDA ATLANTIC UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-06-01 至 2025-05-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10726269
关键词：
Anabolism Animals Anxiety Autoimmune Behavior Behavioral Brain Breeding Cells Cellular biology Central Nervous System Diseases Clinical Research DNA Sequence Epilepsy Erythrocytes Fever Genetic Genetic Recombination Hippocampus Homeostasis IL1B gene IL1R1 gene Immune Immunohistochemistry In Situ Hybridization Inflammatory Interleukin Activation Interleukin-1 Interleukins Internal Ribosome Entry Site Kainic Acid Knock-in Learning Maps Mediating Memory Mental disorders Messenger RNA Methods Modeling Moods Mus Nerve Degeneration Neuroimmune Neurons Oranges Pathogenesis Pathogenicity Peripheral Pharmacotherapy Physiological Population Regulation Reporter Research Role Severities Sleep Slow-Wave Sleep Source Terminator Codon Visualization behavioral outcome cytokine depressive symptoms design designer receptors exclusively activated by designer drugs excitotoxicity genetic manipulation insight invention nervous system disorder neural circuit neurochemistry neuroinflammation preclinical study prevent receptor tool

Anabolism Animals Anxiety Autoimmune Behavior Behavioral Brain Breeding Cells Cellular biology Central Nervous System Diseases Clinical Research DNA Sequence Epilepsy Erythrocytes Fever Genetic Genetic Recombination Hippocampus Homeostasis IL1B gene IL1R1 gene Immune Immunohistochemistry In Situ Hybridization Inflammatory Interleukin Activation Interleukin-1 Interleukins Internal Ribosome Entry Site Kainic Acid Knock-in Learning Maps Mediating Memory Mental disorders Messenger RNA Methods Modeling Moods Mus Nerve Degeneration Neuroimmune Neurons Oranges Pathogenesis Pathogenicity Peripheral Pharmacotherapy Physiological Population Regulation Reporter Research Role Severities Sleep Slow-Wave Sleep Source Terminator Codon Visualization behavioral outcome cytokine depressive symptoms design designer receptors exclusively activated by designer drugs excitotoxicity genetic manipulation insight invention nervous system disorder neural circuit neurochemistry neuroinflammation preclinical study prevent receptor tool

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项目摘要

PROJECT SUMMARY IL-1 is a master neuroimmune regulator. IL-1 activity is important for both physiological functions of the brain and pathogenesis of all CNS diseases. The cellular sources of IL-1 are not well defined and genetic access to manipulating IL-1-expressing cells is lacking. We have created an IL-1-trap mouse to visualize IL-1 expressing cells. In addition, we can inhibit or activate IL- 1-expressing cells by chemogenetic methods. Using this new tool, we will: 1) demonstrate distinct sets of cells express IL-1 in homeostasis and in different neuroimmune activation conditions; 2) determine the role of homeostatic IL-1-expressing cells on the neurochemical and behavioral consequences following peripheral and central inflammatory challenge; and 3) determine the role of the induced IL-1-expressing cells on the neurochemical and behavioral consequences following peripheral and central inflammatory challenges.

项目摘要 IL-1是主神经免疫调节剂。 IL-1活动对于两个物理功能都很重要所有中枢神经系统疾病的大脑和发病机理。 IL-1的细胞来源不好缺乏定义的和遗传操纵表达IL-1的细胞的机会。我们创建了 IL-1捕获小鼠可视化IL-1表达细胞。另外，我们可以抑制或激活IL- 通过化学发生方法表达1细胞。使用此新工具，我们将：1）证明不同的细胞集在体内平衡和不同的神经免疫性激活中表达IL-1 状况; 2）确定稳态表达IL-1细胞在神经化学和周围和中央炎症挑战后的行为后果； 3）确定诱导的表达IL-1细胞在神经化学和行为上的作用外围和中央炎症挑战后的后果。