Comparative genomics of a unique mutualistic apicomplexan

独特的互惠顶端复合体的比较基因组学

基本信息

批准号：
9248239
负责人：
Christopher Lane
金额：
$ 7.26万
依托单位：
UNIVERSITY OF RHODE ISLAND
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-04-01 至 2019-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9248239
关键词：
Address Affinity Agriculture Animals Apical Apicomplexa Babesia Babesiosis Bacteria Biology Calcium Oxalate Cells Characteristics Chordata Coccidiosis Complex Contracts Cryptosporidiosis Cytology Data Dependence Deposition Disease Eimeria Employee Strikes Evolution Family Funding Gene Family Generations Genes Genome Genomics Goals Human Infection Invaded Kidney Laboratories Life Style Livestock Malaria Marine Invertebrates Marines Medical Metabolic Microbe Microscopy Organ Organism Outcome Parasites Pathogenicity Phenotype Phylogenetic Analysis Physiology Planet Earth Plasmodium Play Proteins Purines Research Role Source Structure System Theileria Theileriasis Toxoplasma Toxoplasmosis Urate Urochordata Vertebrates Virulence ascidian base comparative genomics endosymbiont experimental study functional genomics genomic data genomic signature innovation interest invertebrate host member microorganism interaction mutualism parasitism pathogen public health relevance response tool

项目摘要

DESCRIPTION (provided by applicant) Apicomplexa is a group of unicellular organisms that has been long been considered an exclusively parasitic clade. There are many ecologically and medically important apicomplexans, including the causative agent of malaria. However, recent evidence has revealed one group of mutualistic apicomplexans: the marine symbiont genus, Nephromyces. Nephromyces are unusual apicomplexans in many ways. First, members of this genus are found exclusively inside one family of sea squirts, where they colonize a large, ductless organ, the renal sac. Within this organ, Nephromyces surround urate and calcium oxalate deposits. Second, unusually for apicomplexans, Nephromyces cells contain intracellular bacteria. This mutualistic apicomplexan offers a singular opportunity to address the important question about apicomplexan biology. With a long-term goal of completely characterizing this system, here we propose to focus on comparing the Nephromyces genome to its closest relative, the parasitic genus, Cardiosporidium. Members of Cardiosporidium parasitize a broad range of sea squirts yet, like Nephromyces, also contain endosymbiotic bacteria. The genomes of the bacteria inhabiting both Nephromyces and Cardiosporidium will also be sequenced. Data generation will have an emphasis on the hypothesis that the bacterial symbionts may provide key metabolic functions in the evolution of this mutualism. The genome sequences from these organisms will provide a stepping-stone for cytological, microscopy and functional genomic research. Understanding the genomic signatures of lifestyle transitions in Apicomplexa will have significant impact on determining which, as yet underappreciated, genomic features are correlated with virulence and parasitism.

描述（由申请人提供）顶端复合体是一组单细胞生物，长期以来一直被认为是一种独特的寄生进化枝，有许多生态和医学上重要的顶端复合体，包括疟疾的病原体。然而，最近的证据揭示了一组互利的顶端复合体：海洋共生体属。肾霉菌属在很多方面都是不寻常的顶端复合菌，首先，该属的成员仅在海洋中的一个科中发现。其次，肾霉菌细胞中含有胞内细菌，这种共生的顶端复合物提供了一个独特的机会来解决这一问题。关于 apicomplexan 生物学的问题，我们的长期目标是完全表征该系统，在这里我们建议重点比较肾霉菌。与其最近亲，心孢子虫属的成员寄生了多种海鞘，但与肾霉菌属一样，也含有内共生细菌。居住在肾霉菌属和心孢子虫属的细菌的基因组也将被测序。强调细菌共生体可能在这种互利共生的进化中提供关键代谢功能的假设。这些基因组序列。了解 Apicomplexa 生活方式转变的基因组特征将为细胞学、显微镜学和功能基因组研究提供基础，这将对确定哪些基因组特征与毒力和寄生性相关（但尚未得到充分认识）产生重大影响。

项目成果

期刊论文数量（4）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Nephromyces Represents a Diverse and Novel Lineage of the Apicomplexa That Has Retained Apicoplasts.

肾霉菌代表了保留了顶端质体的顶端复合体的多样化和新颖的谱系。

DOI：
发表时间：
2019-10-01
期刊：
影响因子：
0
作者：
Muñoz;Durnin, Keira;Eme, Laura;Paight, Christopher;Lane, Christopher E;Saffo, Mary B;Slamovits, Claudio H
通讯作者：
Slamovits, Claudio H

Nephromyces Encodes a Urate Metabolism Pathway and Predicted Peroxisomes, Demonstrating That These Are Not Ancient Losses of Apicomplexans.

肾霉菌编码尿酸代谢途径和预测的过氧化物酶体，证明这些并不是顶端复合物的古老损失。