Development of a rapid screening test for the detection of dihydroanatoxin-a

开发检测二氢虾毒素-a 的快速筛选试验

基本信息

批准号：
10545266
负责人：
LANCE P FORD
金额：
$ 25.9万
依托单位：
ATTOGENE
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-07-07 至 2025-06-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10545266
关键词：
Address Affect Affinity Anabaena Anatoxins Animals Antibodies Antigens Aphanizomenon Back Binding Biological Assay Breathing Buffers Cardiovascular system Cells Cessation of life Chronic Complex Consumption Convulsions Cyanobacterium Cyanotoxin Deposition Detection Development Devices Disease Outbreaks Early Diagnosis Eating Ensure Environment Exposure to Family Feasibility Studies Food Goals Goat Gold Gold Colloid Health Human Human Resources Immunoglobulin G International Laboratories Liquid substance Membrane Methods Monitor Muscle Muscle fasciculation Nerve Neurologic Neurons Neurotoxins Nicotinic Receptors Nutrient Oryctolagus cuniculus Phase Poisoning Preparation Prevention Procedures Property Protocols documentation Public Health Pyroxylin Rapid screening Reagent Receptor Inhibition Recreation Respiratory Paralysis Running Sampling Sensitivity and Specificity Severities Signal Transduction Site Sodium Chloride Source Swimming Symptoms Techniques Technology Temperature Testing Time Toxic Environmental Substances Toxic effect Toxin Training Tumor Promotion United States Department of Agriculture Validation Variant Water aerosolized chemical synthesis contaminated drinking water cost cost effective detection assay detection test detector field study global environment improved innovation instrumentation lateral flow assay manufacture nanoparticle performance tests phase 2 study prevent programs prototype rapid detection rapid test receptor binding screening tool water sampling

项目摘要

Project Summary Dihydroanatoxin (dhATX) derived from cyanobacteria is a rapidly emerging global problem that negatively impacts human and animal health. Poisoning induced by dhATX is characterized by a broad array of neurological and cardiovascular symptoms with extremes of severity which can lead to fatality. Therefore, efficient, and effective detection, prevention, control, and mitigation technologies are critically needed to prevent exposures. The only available technology to detect the presence of dhATX is expensive, time consuming, and labor intensive, allowing only sporadic testing to be performed. This lack of rapid, simple, inexpensive testing capability increases the chances of human and animal exposures and associated health problems. Based on our current progress in developing rapid assays for environmental toxins, we will develop a rapid, quantitative, and cost- effective receptor binding assay for detecting dhATX in the field setting. Our test platform will likely emerge as an integral component of monitoring programs domestically and internationally to improve dhATX detection, and will provide an effective tool for public health officials to act quickly to reduce dhATX exposures.

项目概要源自蓝藻的二氢虾毒素 (dhATX) 是一个迅速出现的全球性问题，对影响人类和动物健康。 dhATX 引起的中毒具有广泛的神经系统症状以及严重程度可能导致死亡的心血管症状。因此，高效、为防止暴露，迫切需要有效的检测、预防、控制和缓解技术。检测 dhATX 存在的唯一可用技术昂贵、耗时且费力密集的，只允许进行零星的测试。缺乏快速、简单、廉价的测试能力增加人类和动物接触和相关健康问题的机会。根据我们目前的在开发环境毒素快速检测方法方面取得进展，我们将开发一种快速、定量且成本低廉的方法用于在现场检测 dhATX 的有效受体结合测定。我们的测试平台可能会出现国内和国际监测计划的一个组成部分，以改进 dhATX 检测，以及将为公共卫生官员迅速采取行动减少 dhATX 暴露提供有效的工具。