Hand-Held Phased Array:Localization of Molecular Beacons

手持式相控阵：分子信标的定位

• 批准号: 6740032
• 负责人: SHOKO NIOKA
• 金额: $ 14.98万
• 依托单位: OPTICAL DEVICES, INC.
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-02-06 至 2005-08-05
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6740032
• 关键词: bioimaging /biomedical imaging; biomedical equipment development; biosensor device; breast neoplasm /cancer diagnosis; breast neoplasms; cost effectiveness; fluorescent dye /probe; image guided surgery /therapy; ionophores; optics; portable biomedical equipment

DESCRIPTION (provided by applicant): Diagnosis and therapy of small subsurface tumors of breast is rendered difficult by: a) their small size, b) their particular signatures of genetic expression, and c) the difficulty of delivery of therapeutics, specifically to the tumor site. This research and development is directed towards the development of Near Infrared (NIR) localizer (+/-1mm) for tumors that can be 3 or more cm below the surface of the skin and a few mm in diameter. The convergence of two quantities into a single instrument is proposed to achieve these goals. First, with respect to imaging at high localization, the phase interference method (phased array cancellation) has now been developed to the point where a line of sight to the model cancer tissues has been perfected to a pointing accuracy of +/-1mm for a 3cm subsurface object containing 2) sub-nanomolar amounts of a cancer seeking fluorochrome. The device is comprised of a pair of monochromatic light sources suitable for excitation of fluorescence of the particular molecular beacon, i.e., usually at 780nm, and an equidistant single detector for measuring the phase imbalance detected by a secondary filter usually at 830nm. This detector senses phase inequality of the two anti-phase signals with an output at a null of 90 degrees (the mean value of the phase from the 2 sources). A small object will displace the phase null from 90 degrees by a significant amount (up to 20 degrees phase shift per mm displacement of a small object from the null plane of the phased array). Such displacement is to be read from the numerical indicator of the hand-held phased array scanner. In cases where no other localizer is available for biopsy, the line of sight provided by this instrument could be used as a biopsy guide providing the positional information from several sites. Further applications to head and neck cancer and brain cancer are outlined. It is predicted that this hand-held ergonomic battery operated 2-D tumor localizer will be a prototype in the new family of hand-held disease detection devices that afford fast, real time, accurate detection of subsurface pathologies such as molecular beacon labeled cancers, etc., therefore, technical transfer has already been outlined and a wide market may be established.

描述（由申请人提供）： 乳房中较小的地下肿瘤的诊断和治疗难以通过：a）小体积，b）遗传表达的特殊特定签名，c）疗法递送的难度，特别是向肿瘤部位提供。这项研究和发育针对的是近红外（NIR）定位剂（+/- 1mm）的肿瘤，该肿瘤可能以下3厘米以下3 cm或更多CM，直径为几毫米。提出了两个数量到单个工具的融合以实现这些目标。首先，关于在高位置下的成像，现在已经开发了相位干扰方法（分阶段阵列取消）到模型癌组织的视线，以使得含有3厘米的地下对象的+/- 1mm的指向精度为2），含有2）含有癌症的亚nan摩尔量。该设备由一对单色光源组成，适合于特定分子信标的荧光激发，即通常在780nm处，以及一个等距的单个检测器，用于测量通常在830nm时通过二级过滤器检测到的相位不平衡。该检测器感应两个反相信号的相位不等式，其输出为90度（来自两个源的平均值）。一个小物体将相位从90度移位，大量量（从分阶段阵列的无效平面中，每毫米的每毫米位移最多20度相移）。这种位移将从手持式阵列扫描仪的数值指标中读取。如果没有其他定位器可进行活检，则该乐器提供的视线可以用作活检指南，可从多个站点提供位置信息。概述了针对头颈癌和脑癌的进一步应用。据预测，这种手持式人体工程学电池操作的二维肿瘤定位器将是新的手持疾病检测设备的新家族的原型，这些疾病检测设备可提供快速，实时，准确地检测地下病理学，例如分子信标标记的癌症等地下病理。