3-D Image Restoration for Polarized Light Microscopy

偏光显微镜的 3D 图像恢复

基本信息

批准号：
6656953
负责人：
RUDOLF OLDENBOURG
金额：
$ 25.29万
依托单位：
MARINE BIOLOGICAL LABORATORY
依托单位国家：
美国
项目类别：
财政年份：
2001
资助国家：
美国
起止时间：
2001-09-15 至 2004-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6656953
关键词：
automated data processing bioengineering /biomedical engineering bioimaging /biomedical imaging birefringences cell component structure /function computer program /software image processing light microscopy mathematics optical polarization structural biology

项目摘要

DESCRIPTION (provided by applicant): We have been developing and applying a new type of polarized light microscope (the P01-Scope) which dramatically enhances the analytic power of the traditional polarizing microscope. The P01-Scope incorporates a universal compensator, made from computer driven liquid crystal devices, to measure the birefringent fine structure at high sensitivity (0.02 nm birefringence retardation), high resolution (0.2 pm), simultaneously over the whole field of view, and for all orientations of the birefringence axis within the focus plane. A 3-dimensional birefringent specimen, such as a living cell, is imaged with the Pol-Scope as a series of optical Sections. Each section contains the birefringence of in-focus structures, such as membranous stacks, aligned filament arrays and individual filament bundles. However, the measurements of in-focus birefringences have spurious contributions from out-of focus cellular components. The structural analysis of the detailed and quantitative information afforded by the P01-Scope is often limited because of these spurious birefringence contributions. Therefore, to take full advantage of the quantitative Pol-Scope images, the out-of-focus information needs to be identified and removed. We are proposing to develop a computational restoration procedure to remove the effect of out-of-focus components on the measurements of in-focus birefringences. The P01-Scope restoration algorithm will be based on procedures established for fluorescence microscopy that we will modify to account for the specific properties of partial coherent imaging with polarized light. The first development goal is to establish a forward procedure that is used to compute image features based on measured point spread functions and known or estimated positions, magnitudes and axis orientations of birefringent objects. The second goal is to find the inverse procedure that optimizes a numerical estimate of object birefringences in volume elements that are contained in P01-Scope optical sections. The inverse procedure iteratively minimizes the difference between experimental and computed images. This process ends in a stable 3-D distribution of positions, magnitudes and axis orientations of specimen birefringences. The image restoration procedures will greatly enhance the utility of the P01-Scope for biological and medical applications to non-invasively analyze the architectural dynamics of living cells.

描述（由申请人提供）：我们一直在开发和应用新的极化光学显微镜（P01-SCOPE）的类型，可显着增强传统偏振显微镜的分析能力。 P01-SCOPE 结合了由计算机驱动液晶制成的通用补偿器设备，以高灵敏度测量双折射良好结构（0.02 NM双重智障），高分辨率（0.2 pm），同时超过整个视野，以及双方轴的所有方向在聚焦平面内。一个三维的双折射标本，例如居住细胞与波波式成像为一系列光学截面。每个部分包含聚焦结构的双折射，例如膜堆栈，对齐的灯丝阵列和单个灯丝束。但是，对重点双折射的测量结果有虚假的贡献聚焦细胞成分。详细的结构分析 P01-SCOPE提供的定量信息通常受到限制这些虚假的双折射贡献。因此，充分利用在定量的pol-scope图像中，需要进行异位信息确定并删除。我们建议开发计算恢复删除焦点组件对测量的效果的程序焦点双折射。 P01-Scope Restoration算法将基于根据荧光显微镜确定的程序，我们将修改为考虑与极化的部分相干成像的特定特性光。第一个发展目标是建立一个远期程序用于根据测量点扩展功能计算图像特征和双折射的已知或估计位置，大小和轴向取向对象。第二个目标是找到优化一个的逆过程卷元素中对象双折射的数值估计包含在P01-Scope光学部分中。反向过程迭代最小化实验图像和计算图像之间的差异。这个过程以稳定的位置，幅度和轴的稳定3-D分布结束标本双向框的方向。图像修复程序将极大地增强了P01-SCOPE的生物学和医疗效用非侵入性分析生活的建筑动力学的应用细胞。

项目成果

期刊论文数量（5）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Polarized light microscopy in reproductive and developmental biology.

DOI：
10.1002/mrd.22221
发表时间：
2015-07
期刊：
Molecular reproduction and development
影响因子：
2.5
作者：
Koike-Tani M;Tani T;Mehta SB;Verma A;Oldenbourg R
通讯作者：
Oldenbourg R

pH dependent isotropic to nematic phase transitions in graphene oxide dispersions reveal droplet liquid crystalline phases.

DOI：
10.1039/c4cc00970c
发表时间：
2014-05
期刊：
Chemical communications
影响因子：
4.9
作者：
Rachel Tkacz;R. Oldenbourg;Shalin B. Mehta;Morteza Miansari;Amitabh Verma;M. Majumder
通讯作者：
Rachel Tkacz;R. Oldenbourg;Shalin B. Mehta;Morteza Miansari;Amitabh Verma;M. Majumder

Polarized light microscopy: principles and practice.

偏光显微镜：原理与实践。