Instantaneous 3D imaging of cells and tissues using polarized light microscopy

使用偏光显微镜对细胞和组织进行瞬时 3D 成像

• 批准号: 9527370
• 负责人: RUDOLF OLDENBOURG
• 金额: $ 10.91万
• 依托单位: MARINE BIOLOGICAL LABORATORY
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-05-01 至 2019-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9527370
• 关键词: 3-Dimensional; Accounting; Algorithmic Software; Algorithms; Anisotropy; Architecture; Area; Biological; Biological Models; Biology; Birefringence; Cell division; Cells; Chromosomes; Clinical; Clinical Research; Collaborations; Collagen; Computer software; Data; Development; Device or Instrument Development; Disease; Eukaryotic Cell; Fibroblasts; Fluorescence; Fluorescence Polarization; Grant; Health; Image; Investigation; Laboratory Research; Length; Light; Maps; Measures; Medical Research; Medicine; Meiosis; Methods; Microscope; Microscopy; Molecular; Molecular Analysis; Nature; Optics; Pattern; Photophobia; Polarization Microscopy; Polymers; Process; Property; Refractive Indices; Resolution; Shapes; Solid; Specimen; Spermatocytes; Structure; Techniques; Technology; Theoretical Studies; Three-Dimensional Imaging; Time; Tissue imaging; Tissues; Uncertainty; absorption; attenuation; beam-splitter; cellular imaging; design; experimental study; extracellular; fluorescence imaging; fly; imaging modality; insight; instrument; instrumentation; liquid crystal; microscopic imaging; new technology; novel strategies; polarized light; public health relevance; reconstruction; repaired; sensor; submicron; tomography; vector; web portal

 DESCRIPTION (provided by applicant): We are proposing to develop the polarized light field microscope for analyzing the architectural dynamics inside living cells, tissues and cell-free model systems. The instrument combines comprehensive polarization analysis and 3D imaging of microscopic specimens in a single snapshot using the new approach of light field imaging. The project includes the development of the hardware, software and algorithms required for capturing polarized light field images and their tomographic reconstruction into 3-dimensional maps of birefringence, diattenuation and polarized fluorescence. The instrument development is guided by several application projects including the remodeling of the extracellular collagen matrix by fibroblast cells, the spatial coordination in the assembly of higher order septin structures inside eukaryotic cells and the origins of chromosome malorientations and their repair in the meiotic spindle of spermatocytes. Information about the technology, including software components and instrument design that are developed under this grant, will be made available for users and developers on an established web portal called OpenPolScope.org.

 描述（通过应用程序证明）：我们提议开发用于分析活细胞，组织和自由模型系统的偏光光场显微镜。光场成像。染色体恶意的真核细胞和ermatocyte的细胞中的sple，包括软件组件和仪器设计。

项目成果

Analysis of Septin Reorganization at Cytokinesis Using Polarized Fluorescence Microscopy.

使用偏光荧光显微镜分析胞质分裂时的 Septin 重组。

• DOI: 10.3389/fcell.2017.00042
• 发表时间: 2017
• 期刊: Frontiers in cell and developmental biology
• 影响因子: 5.5
• 作者: McQuilken,Molly;Jentzsch,MaximilianS;Verma,Amitabh;Mehta,ShalinB;Oldenbourg,Rudolf;Gladfelter,AmyS
• 通讯作者: Gladfelter,AmyS

Single-fluorophore orientation determination with multiview polarized illumination: modeling and microscope design.

使用多视图偏振照明确定单荧光团方向：建模和显微镜设计。

• DOI: 10.1364/oe.25.031309
• 发表时间: 2017
• 期刊: Optics express
• 影响因子: 3.8
• 作者: Chandler,Talon;Mehta,Shalin;Shroff,Hari;Oldenbourg,Rudolf;LaRivière,PatrickJ
• 通讯作者: LaRivière,PatrickJ

Achiral symmetry breaking and positive Gaussian modulus lead to scalloped colloidal membranes

• DOI: 10.1073/pnas.1617043114
• 发表时间: 2017-04-25
• 期刊: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• 影响因子: 11.1
• 作者: Gibaud, Thomas;Kaplan, C. Nadir;Dogic, Zvonimir
• 通讯作者: Dogic, Zvonimir