3D imaging of cells and tissues using polarized light microscopy

使用偏光显微镜对细胞和组织进行 3D 成像

• 批准号: 10597635
• 负责人: RUDOLF OLDENBOURG
• 金额: $ 50.18万
• 依托单位: MARINE BIOLOGICAL LABORATORY
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-06-01 至 2025-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10597635
• 关键词: 3-Dimensional; Algorithms; Architecture; Biological; Birefringence; Cell division; Cells; Clinical; Collagen; Computer software; Data; Development; Device or Instrument Development; Disease; Eukaryotic Cell; Fibroblasts; Fluorescence Polarization; Grant; Health; Image; Imaging Techniques; Light; Light Microscope; Lighting; Maps; Measurement; Medical Research; Microscope; Molecular; Polarization Microscopy; Process; Resolution; Structure; Techniques; Technology; Three-Dimensional Imaging; Time; Tissues; Whole Organism; cellular imaging; design; extracellular; instrument; instrumentation; polarized light; reconstruction; tomography; web portal

Project Summary We are proposing to continue our development of polarized light microscopy techniques for analyzing the architectural dynamics inside living cells, tissues and whole organisms. We propose to add comprehensive polarization analysis to two multi-view imaging techniques, the dual-view plane illumination microscope, or diSPIM, and the light-field microscope. Both types of polarized light microscopes represent significant new developments that for the first time enable the measurement of molecular orientation parameters in three dimensions at high spatial (0.5µm in all three dimensions) and temporal (faster than 1Hz) resolution. The project includes the development of the hardware, software and algorithms required for capturing multi-view image data and their tomographic reconstruction into 3-dimensional maps of birefringence and polarized fluorescence. The instrument development is guided by several application projects including the remodeling of the extracellular collagen matrix by fibroblast cells and the spatial coordination in the assembly of higher order septin structures inside eukaryotic cells. Information about the technology, including software components and instrument design that are developed under this grant, will be made available for users and developers on an established web portal called OpenPolScope.org.

项目摘要 我们建议继续开发两极光显微镜技术，以分析 活细胞，组织和整个生物体内部的建筑动力学。我们建议添加全面 对两种多视图成像技术的极化分析，即双视平面照明显微镜或 DeSpim和光场显微镜。两种类型的极化光显微镜代表着重要的新 首次实现三个分子方向参数的开发 高空间（在所有三个维度为0.5µm）和暂时分辨率的尺寸（比1Hz更快）。这 项目包括开发捕获多视图所需的硬件，软件和算法 图像数据及其断层造影重建为双重折线和极化的3维图 荧光。仪器开发的指导下的几个申请项目，包括重塑 成纤维细胞的细胞外胶原基质和高阶组装中的空间配位 真核细胞内的隔蛋白结构。有关该技术的信息，包括软件组件和 根据该赠款开发的仪器设计，将为用户和开发人员提供 建立的Web门户名为openpolscope.org。