QUANTITATIVE 3D ULTRASOUND IMAGING OF THE MURINE HEART

小鼠心脏的定量 3D 超声成像

• 批准号: 6708647
• 负责人: John A Hossack
• 金额: $ 30.97万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-09-05 至 2007-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6708647
• 关键词: bioimaging /biomedical imaging; biomedical equipment development; contrast media; heart imaging /visualization /scanning; image enhancement; image processing; intracardiac volume; laboratory mouse; magnetic resonance imaging; myocardial infarction; phantom model; three dimensional imaging /topography; ultrasound blood flow measurement

DESCRIPTION (provided by applicant): The mouse has become the preferred species for cardiovascular research into the genetic mechanisms that underpin cardiovascular disease. In addition, mice are increasingly being used to study the evolution of anatomic and physiological responses to disease and therapy. The availability of transgenic and "knockout" mice, combined with conventional pharmacologic approaches, make the mouse a uniquely powerful species in which to study cardiovascular disease. One current method for non-invasive mouse imaging (MRI) has excellent image quality but its potential for widespread application is limited by its high cost, poor temporal resolution and low throughput. We propose an ultrasound method that provides accurate, low-cost, fast and non-invasive quantification of cardiac left ventricular (LV) volumes and function in small animals. Additionally, it is easy-to-use, requires minimal mouse preparation and minimal scanning time. The spatial resolution is sufficient to enable calculation of important anatomic and physiologic parameters (chamber volumes, ejection fraction, cardiac output, etc.) Furthermore, we take advantage of the superior temporal resolution to enable assessment of mouse LV perfusion using analysis of the time evolution of myocardial video intensity following bolus injection of microbubble contrast agents. The Specific Aims of this project are to: 1) Develop a dedicated murine ultrasound transducer array / scanner pair capable of a spatial resolution of 200 microns laterally and 100 microns axially with a frame rate of 100+ frames per second. It is believed that this may be the first phased array transducer optimized specifically for mouse heart imaging. 2) Develop a volumetric (3D) ultrasound scanner, specially matched for murine heart imaging, based on the array in Aim 1. Using positional knowledge of the acquired 2D image frames, we will interpolate to form a 3D volumetric data set. 3) Expand the capability and utility of the murine heart scanner to include advanced image processing, novel approaches to automatic LV border detection and include quantitative analysis of contrast agent-based perfusion images. 4) Perform a validation study in vitro using an ultrasound phantom and in vivo in infarcted mouse hearts using Magnetic Resonance Imaging (MRI) as a standard.

描述（由申请人提供）：小鼠已成为对基于心血管疾病的遗传机制进行心血管研究的首选物种。此外，小鼠越来越多地用于研究解剖和生理对疾病和治疗的反应的演变。转基因和“基因敲除”小鼠的可用性，结合常规的药理方法，使小鼠成为研究心血管疾病的独特功能。一种非侵入性小鼠成像（MRI）的当前方法具有出色的图像质量，但其广泛应用的潜力受其高成本，时间分辨率差和低通量的限制。我们提出了一种超声方法，该方法可提供精确，低成本，快速和非侵入性定量对小动物的心脏左心室（LV）体积和功能。此外，它易于使用，需要最少的小鼠准备时间和最少的扫描时间。空间分辨率足以启用重要的解剖和生理参数（腔室量，射血分数，心脏输出等），我们还利用了较高的时间分辨率来评估小鼠LV灌注，并利用在胶体注射小量后进行微生物的时间进化来分析Microbububble the Microbubble the Microbubble Ambents的时间演化。该项目的具体目的是： 1）开发一个专用的鼠超声换能器阵列 /扫描仪对，能够横向分辨率为200微米，轴向100微米，每秒帧速率为100+帧。人们认为，这可能是专门针对小鼠心脏成像优化的第一个分阶段阵列传感器。 2）基于AIM 1中的阵列，开发一个体积（3D）超声扫描仪，专门匹配鼠心成像。使用获得的2D图像帧的位置知识，我们将插入以形成3D体积数据集。 3）扩展了鼠心脏扫描仪的能力和效用，包括高级图像处理，自动LV边框检测的新方法，并包括对基于造影剂的灌注图像的定量分析。 4）在体外使用超声幻影和体内在梗塞小鼠心脏中使用磁共振成像（MRI）作为标准进行验证研究。