Cardiorenal and Metabolic Diseases Research Center

心肾与代谢疾病研究中心

基本信息

批准号：
10887793
负责人：
John E Hall
金额：
$ 25万
依托单位：
UNIVERSITY OF MISSISSIPPI MED CTR
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-07-15 至 2028-06-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10887793
关键词：
Area Biopsy Cardiorenal syndrome Cardiovascular Diseases Cell Culture Techniques Centers of Research Excellence Chronic Disease Clinical Complex DNA Sequence Data Dedications Disease Ensure Equipment Fluorescent Antibody Technique Funding Future Genetic Transcription Genomics Grant Health Kidney Diseases Knowledge Light Link Measures Medical center Metabolic Diseases Methods Mississippi Molecular Molecular Chaperones National Institute of General Medical Sciences Parents Patients Peptides Phosphorylation Population Productivity Protein Kinase Proteins Proteomics Regulation Regulatory Element Reporter Research Research Personnel Role Sampling Scientist Set protein Structural Protein System Technology Tissues United States National Institutes of Health Universities base complex biological systems equipment acquisition improved innovation multidisciplinary new technology protein expression time use transcription factor transcriptomics

项目摘要

PROJECT SUMMARY/ABSTRACT Cardiovascular (CV), renal and metabolic diseases are highly prevalent in the U.S., especially in Mississippi. We established the Cardiorenal and Metabolic Diseases Research Center (CMDRC) at the University of Mississippi Medical Center to bring together a multidisciplinary group of basic, clinical and population scientists working on CV, renal, and metabolic diseases. Understanding the complex relationship between these chronic diseases requires the combined efforts of basic, clinical and population scientists using innovative multidisciplinary integrative approaches to research and state-of-the-art technologies that facilitate discovery. The molecular bases for interdependence of CV, renal, and metabolic diseases have classically been studied via genomics, transcriptomics, and proteomics platforms that assess DNA sequences and levels of RNA and protein expression. While data generated from these studies are informative, regulation of complex biological systems also requires understanding protein activity. Protein kinases are central to governing protein activity networks and link several regulatory elements, including transcription factors, chaperones, and structural proteins. The kinome refers to the broad-based activity of the complete set of protein kinases. Several methods have recently been developed to study the kinome in light of the vast activity of protein kinases. One of these new technologies is the PamChip which uses hundreds of ~13 residue-long reporter peptides which are phosphorylated with samples derived from cell cultures, tissues, or clinical biopsies. The degree of phosphorylation is measured in real-time using fluorescent antibodies. This technology is encompassed in the PAMStation 12 from Pamgene. This equipment supplement application is for funds to purchase a PAMStation 12 for the CMDRC. The acquisition of this state-of-the-art system will allow investigators of the parent COBRE to determine the role of the kinome in the areas of CV, renal, and metabolic diseases. This new equipment will also increase the sophistication of experimental approaches and strengthen future NIH grants from CMDRC investigators as well as other investigators at UMMC, including those supported by other NIGMS funded IDeA centers. The CMDRC has been a major driver for marked expansion of cardiorenal and metabolic diseases research in Mississippi. This large equipment grant will amplify and ensure sustainability of our efforts to develop a highly productive CMDRC dedicated to improving lives through research, discovery, and innovation.

项目概要/摘要心血管（CV）、肾脏和代谢疾病在美国非常普遍，尤其是在密西西比州。我们在密西西比大学建立心肾和代谢疾病研究中心（CMDRC）医学中心汇集了由基础、临床和人口科学家组成的多学科小组，致力于研究心血管、肾脏和代谢疾病。了解这些慢性疾病之间的复杂关系需要基础科学家、临床科学家和人口科学家利用创新的多学科的共同努力综合研究方法和促进发现的最先进技术。分子传统上通过基因组学研究了心血管、肾脏和代谢疾病相互依赖性的基础，评估 DNA 序列以及 RNA 和蛋白质水平的转录组学和蛋白质组学平台表达。虽然这些研究产生的数据信息丰富，但复杂生物系统的调控还需要了解蛋白质活性。蛋白激酶是控制蛋白质活性网络的核心并连接多个调控元件，包括转录因子、分子伴侣和结构蛋白。这激酶组是指整套蛋白激酶的广泛活性。最近有几种方法鉴于蛋白激酶的巨大活性，已开发用于研究激酶组。其中一项新技术是 PamChip，它使用数百个约 13 个残基长的报告肽，这些肽被磷酸化来自细胞培养物、组织或临床活组织检查的样品。磷酸化程度的测量实时使用荧光抗体。 Pamgene 的 PAMStation 12 中包含该技术。该设备补充申请旨在为CMDRC购买PAMStation 12提供资金。此次收购这种最先进的系统将使母体 COBRE 的研究人员能够确定激酶组的作用在心血管、肾脏和代谢疾病领域。这种新设备还将提高实验方法并加强来自 CMDRC 研究人员以及其他人的未来 NIH 资助 UMMC 的研究人员，包括其他 NIGMS 资助的 IDeA 中心支持的研究人员。 CMDRC 已密西西比州心肾和代谢疾病研究显着扩展的主要推动力。这个大设备补助将扩大并确保我们发展高生产力 CMDRC 的努力的可持续性致力于通过研究、发现和创新改善生活。