Vet-LIRN Network Capacity-Building Project: Bacterial Whole Genome Sequencing

Vet-LIRN 网络能力建设项目：细菌全基因组测序

• 批准号: 10828232
• 负责人: Udeni B. R. Balasuriya
• 金额: $ 7.5万
• 依托单位: LOUISIANA STATE UNIV A&M COL BATON ROUGE
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-07-01 至 2028-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10828232
• 关键词: Vet; LIRN; Network; Capacity; Building

Summary/Abstract: The need to increase Whole-genome sequencing (WGS) to provide antibiotic resistance genotypic testing in laboratory networks for emerging antibiotic resistance pathogens is critical for the current One Health Concept. Antimicrobial resistance is a constant and growing threat to human and animal health. WGS is one of the recent and more powerful tools to study and monitor the development and progression of the emergence of antibiotic-resistant pathogens. Also, WGS provides real-time sequence data used to build public genomic databases that aid in identifying the genetic characterization of foodborne bacterial pathogens, isolates from clinical specimens, and investigation of outbreaks in health care facilities and to provide information for infection control practices. The continued investment in new technologies such as WGS is crucial for supporting an expanding testing volume while sustaining rapid detection and public health response. Sequencing has also been shown to be an excellent utility for epidemiological investigations of foodborne disease outbreaks, for establishing antimicrobial-resistant dashboards, and for use by clinicians for making the best treatment decisions. The Louisiana Animal Disease Diagnostic Laboratory (LADDL) serves as a whole genome sequencing (WGS) laboratory for the Center for Veterinary Medicine (CVM) Vet-LIRN Veterinary Diagnostic Laboratory Program. LADDL is one of five laboratories originally selected by Vet-LIRN to participate in a pilot project using WGS to monitor antimicrobial resistance in zoonotic and emerging pathogens. Long term goals of this proposal are to add whole genomic sequence genomic data from bacterial isolates originating from animal clinical specimens or animal feed bacterial isolates to National Antimicrobial Resistance Monitoring System and to develop a rapid response network for accurate resolution of putative animal foodborne disease outbreaks. The project has three specific aims. First, to provide the funding required for continuation of Vet- LIRN’s pilot Antimicrobial Resistance (AMR) and Sequencing Project. The primary cost of the project is for purchasing supplies and reagents and for payment of equipment maintenance contracts. Second, to perform WGS on isolates submitted by the Vet-LIRN source labs as genomic information for identifying antimicrobial-resistant bacteria or pathogens related to surveillance or disease outbreaks. Finally, to populate and contribute sequence data to the national databases to aid in the genomic and biomedical information needed to track bacterial pathogens of interest to the CVM and U.S. Food and Drug Administration (FDA).

摘要/摘要： 需要增加全基因组测序（WGS）以提供抗生素耐药性 实验室网络中的基因型测试，用于新兴抗生素耐药性病原体对 当前的健康概念。 人类和动物健康。 WGS的抗生素耐药病原体的出现和进展 提供用于构建公共基因组数据的实时序列数据，有助于识别 食源性细菌病原体的遗传表征，从临床标本中分离出来， 并调查医疗机构中的Outbeaks并提供感染信息 控制惯例。 支持扩大的测试量，同时维持快速检测和公共卫生 响应也已证明是流行病学的绝佳实用性 对食物传播疾病暴发的调查，用于建立抗菌剂 仪表板和临床医生的最佳特征决定 动物疾病诊断实验室（LADDL）用作整个基因组测序（WGS） 中心实验室 实验室计划。 参与使用WGS的试点项目，以监测人畜共患病和 新兴病原体。 来自动物临床标本或动物饲料的双作用分离株分离株的基因组数据 细菌分离株与国家抗菌素耐药性监测系统，并发展快速 响应网络准确解决假定的动物源疾病暴发 项目首先具有三个特定的目标。 LIRN的试点抗菌抗性（AMR）和测序项目。 该项目用于购买用品和试剂，用于支付设备维护 合同第二，在兽医源实验室提交的隔离株上执行WGS 用于鉴定抗菌细菌或与病原体有关的基因组信息 监视或疾病爆发。 国家数据库，以帮助追踪基因组和生物医学信息 CVM和美国食品和药物管理局（FDA）感兴趣的病原体。