Vet-LIRN Network Capacity-Building Project Molecular Gel Imaging system at UI VDL

Vet-LIRN 网络能力建设项目 UI VDL 分子凝胶成像系统

• 批准号: 10828117
• 负责人: Leyi Wang
• 金额: $ 1.24万
• 依托单位: UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-01 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10828117
• 关键词: Vet; LIRN; Network; Capacity; Building

Project Abstract The University of Illinois (UI) Veterinary Diagnostic Laboratory (VDL) is a full service, AAVLD accredited, all species, reference veterinary diagnostic laboratory. As a member of the VET-LIRN, UI VDL is one of the labs actively involved in several VET-LIRN activities including iSeq-sequencing Listeria project as a lead lab, providing isolates for MiSeq WGS sequencing project as a source lab, participation on antimicrobial resistance pilot project, and evaluation of automatic nucleic acid extraction methods as a lead lab. The quality of gel images for conventional molecular PCRs are critical in making call on the results and our current gel imaging system is very old, thus producing low quality of images. In this proposal, we request funding to purchase a Bio-Rad GelDoc Go Gel Imaging System to enhance UI VDL molecular service quality for testing animal diseases, and routine case investigation and surveillance coordinated by Vet-LIRN.

项目摘要 伊利诺伊大学 (UI) 兽医诊断实验室 (VDL) 提供全方位服务，获得 AAVLD 认证，所有 物种，参考兽医诊断实验室。作为 VET-LIRN 的成员，UI VDL 是实验室之一 积极参与多项 VET-LIRN 活动，包括作为牵头实验室的 iSeq 测序李斯特菌项目， 作为源实验室为 MiSeq WGS 测序项目提供分离株，参与抗菌素耐药性研究 试点项目，并作为牵头实验室评估自动核酸提取方法。凝胶图像的质量 传统的分子 PCR 对结果至关重要，我们当前的凝胶成像系统 非常旧，因此产生的图像质量较低。在此提案中，我们请求资金购买 Bio-Rad GelDoc Go 凝胶成像系统可提高动物疾病检测的 UI VDL 分子服务质量，以及 由 Vet-LIRN 协调的例行病例调查和监测。