Vet-LIRN Network Capacity Building for SARS-CoV-2: equipment for analysis of NGS library and data

Vet-LIRN 网络针对 SARS-CoV-2 的能力建设：用于分析 NGS 库和数据的设备

• 批准号: 10827796
• 负责人: Leyi Wang
• 金额: $ 5.28万
• 依托单位: UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-07-01 至 2024-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10827796
• 关键词: Vet; LIRN; Network; Capacity; Building

Project Abstract The University of Illinois (UI) Veterinary Diagnostic Laboratory (VDL) is a full service, AAVLD accredited, all species, reference laboratory. UI VDL is a member of the VET-LIRN. As of Feb 17, 2023, UI VDL has tested 3,287 samples for SARS-CoV-2 and 350 samples of zoo animals in 13 different zoos were tested positive. Next- generation sequencing (NGS) is very useful in case investigation of SARS-CoV-2 in the veterinary diagnostic laboratory and could be affected by many factors including selection of sequencing methods, sequencing library quality, amount of viral genome, sample quality, sample pooling, and bioinformatics analysis. New equipment for analyzing both NGS library and big data will significantly increase our SARS-CoV-2 testing capacity and service quality for routine case diagnosis and surveillance coordinated by Vet-LIRN, thus enhancing Vet-LIRN network laboratory for SARS-CoV-2 testing.

项目摘要 伊利诺伊大学 (UI) 兽医诊断实验室 (VDL) 提供全方位服务，获得 AAVLD 认证，所有 物种，参考实验室。 UI VDL 是 VET-LIRN 的成员。截至2023年2月17日，UI VDL已测试 13 个不同动物园的 3,287 份 SARS-CoV-2 样本和 350 份动物园动物样本检测呈阳性。下一个- 世代测序 (NGS) 在兽医诊断中的 SARS-CoV-2 病例调查中非常有用 实验室，可能受到多种因素的影响，包括测序方法的选择、测序文库 质量、病毒基因组数量、样本质量、样本池和生物信息学分析。新设备 用于分析 NGS 文库和大数据将显着提高我们的 SARS-CoV-2 检测能力 由 Vet-LIRN 协调的常规病例诊断和监测的服务质量，从而提高 Vet-LIRN 用于 SARS-CoV-2 测试的网络实验室。