Visium spatial transcriptome analysis add on to the current proposed scRNA seq analysis

Visium 空间转录组分析添加到当前提出的 scRNA seq 分析中

• 批准号: 10829137
• 负责人: Jianjun Zhao
• 金额: $ 13.66万
• 依托单位: CLEVELAND CLINIC LERNER COM-CWRU
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-08-01 至 2026-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10829137
• 关键词: APEXL2 Gene; Acute Renal Failure with Renal Papillary Necrosis; Affect; Antisense Oligonucleotide Therapy; Cell Communication; Cells; Chromosome Mapping; Cisplatin; Complex; Gene Expression; Gene Expression Profile; Gene Expression Profiling; Immune response; Individual; Kidney; Modeling; Molecular; Morbidity - disease rate; Process; Research; Resolution; Spatial Distribution; Techniques; Tissues; cell type; histological image; interest; mortality; response; single-cell RNA sequencing; therapeutic target; transcriptome; transcriptome sequencing; transcriptomics; treatment response

ABSTRACT: This application is being submitted in response to the Notice of Special Interest (NOSI) identified as NOT-CA-23-045. Cisplatin induced Acute kidney injury (C-AKI) is a complex condition with significant morbidity and mortality. Our research team aims to investigate the cellular and molecular mechanisms involved in AKI and therapy response. We have used single-cell RNA sequencing (scRNA seq) to investigate gene expression levels in individual cells. However, scRNA seq lacks spatial context, which is important for understanding cell-cell interactions and the microenvironment. Therefore, we propose adding Visium Spatial Transcriptomics, a cutting-edge technique that combines histological imaging with RNA sequencing, to our study. We aim to analyze gene expression levels in a spatially resolved manner, identify new cell types and their spatial distribution, and generate high-resolution maps of gene expression patterns in kidney tissue. To investigate the spatial expression patterns of genes involved in the immune response and other processes affected by APE2-ASO treatment, we propose a study using Visium Spatial Transcriptomics in the APE2 ASO-treated cisplatin-induced AKI model. By adding Visium Spatial Transcriptomics to our ongoing AKI study, we aim to gain a deeper understanding of the cellular and molecular mechanisms involved and identify potential therapeutic targets.

抽象的： 该申请是根据已确定的特殊利益通知（NOSI）提交的 作为非CA-23-045。 顺铂诱导的急性肾损伤（C-AKI）是一种复杂的疾病，具有明显的发病率和 死亡。我们的研究小组旨在研究涉及的细胞和分子机制 在AKI和治疗反应中。我们已经使用了单细胞RNA测序（SCRNA SEQ） 研究单个细胞中的基因表达水平。但是，scrna seq缺乏空间上下文， 这对于理解细胞 - 细胞相互作用和微环境很重要。所以， 我们提出添加visium空间转录组学，这是一种结合的尖端技术 通过RNA测序进行组织学成像，我们的研究。我们旨在分析基因表达 以空间解决的方式级别，确定新的细胞类型及其空间分布，以及 在肾脏组织中生成基因表达模式的高分辨率图。调查 与免疫反应和其他过程有关的基因的空间表达模式 在APE2-ASO处理的影响下，我们提出了一项研究，使用visium v​​isium空间转录组学中的研究 APE2 ASO处理的顺铂诱导的AKI模型。通过添加空间转录组学 我们正在进行的AKI研究，我们旨在更深入地了解细胞和分子 涉及的机制并确定潜在的治疗靶标。