Center for Cellular Metabolism Research in Oklahoma

俄克拉荷马州细胞代谢研究中心

• 批准号: 10797920
• 负责人: Lijun Xia
• 金额: $ 25万
• 依托单位: OKLAHOMA MEDICAL RESEARCH FOUNDATION
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-02-05 至 2026-01-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10797920
• 关键词: Administrative Supplement; Aging; Area; Biology; Cardiovascular system; Centers of Research Excellence; Confocal Microscopy; Disease; Equipment; Funding; Image; Mentors; Metabolism; Microscope; Oklahoma; Pathogenesis; Phase; Research; Research Personnel; Research Project Grants; Resolution; Sampling; Scanning; Services; Signal Transduction; Speed; Time; cost; improved; notch protein; programs; success

PROJECT SUMMARY/ABSTRACT Altered cellular metabolism contributes to the pathogenesis of many common diseases that are prevalent in Oklahoma. This Center for Cellular Metabolism Research in Oklahoma (CMRO) Phase I COBRE aims to create and nurture excellence in cellular metabolism in Oklahoma. Two of the current CMRO COBRE Research Project Leaders (RPLs) (Drs. Lee and Cleuren) and two of our graduating RPLs (Drs. Yu and Zhao) use confocal microscopy for their research programs. Additionally, five CMRO COBRE mentors (Drs. Xia, Griffin, Olson, Srinivasan, and Kovats) routinely use confocal microscope for their research. A Nikon C2+ confocal microscope that was purchased in 2017 to support eight investigators at the Cardiovascular Biology Research Program of OMRF is currently used by thirteen investigators, nine of whom are associated with COBRE (69.2%). The RPLs and their mentors are the heaviest users of the C2+ confocal and have relied on this equipment to produce top-notch images for their research at no cost. However, the C2+ confocal is starting to show evidence of aging and overuse in the form of increasing numbers of service calls and down time. Furthermore, the newer confocal microscopes have many technical capabilities that are not available in the C2+ confocal. For example, the Nikon AXR microscope is capable of scanning 2X larger area of the sample at 16X higher resolution and 2X higher sensitivity to the signal. The AXR can also acquire images at a much faster speed, which is ideal for larger samples and live imaging. Additionally, the AXR is capable of deconvoluting the acquired images and improving the resolution even further. This supplement requests partial support for a new Nikon AXR confocal microscope. OMRF will provide 50% of the total cost for the equipment. The requested equipment will reduce the demand on the C2+ confocal, supporting our COBRE RPLs in building their competitive research programs and transitioning to independence.

项目概要/摘要 细胞代谢的改变导致许多常见疾病的发病机制 俄克拉荷马州。俄克拉荷马州细胞代谢研究中心 (CMRO) I 期 COBRE 旨在 在俄克拉荷马州创造和培育卓越的细胞代谢。 两位现任 CMRO COBRE 研究项目负责人 (RPL)（Lee 博士和 Cleuren 博士）以及我们的两位 即将毕业的 RPL（余博士和赵博士）在他们的研究项目中使用共焦显微镜。另外，五 CMRO COBRE 导师（Xia 博士、Griffin、Olson、Srinivasan 和 Kovats）经常使用共焦显微镜 为了他们的研究。 2017 年购买的 Nikon C2+ 共焦显微镜，用于支持八名研究人员 OMRF 心血管生物学研究计划目前由 13 名研究人员使用，其中 9 名 与 COBRE 有关的人 (69.2%)。 RPL 及其导师是 C2+ 的最大用户 共焦，并依靠该设备免费为他们的研究生成一流的图像。 然而，C2+ 共焦开始显示出老化和过度使用的迹象，其形式是增加 服务呼叫次数和停机时间。此外，较新的共焦显微镜具有许多技术 C2+ 共焦不具备的功能。例如，尼康 AXR 显微镜能够 以 16 倍高分辨率和 2 倍信号灵敏度扫描 2 倍大的样品区域。这 AXR 还可以以更快的速度获取图像，这非常适合较大的样本和实时成像。 此外，AXR 能够对获取的图像进行去卷积，甚至提高分辨率 更远。本补充要求对新型 Nikon AXR 共焦显微镜提供部分支持。 OMRF 将 提供设备总成本的50%。所请求的设备将减少对 C2+ 的需求 共聚焦，支持我们的 COBRE RPL 建立有竞争力的研究项目并过渡到 独立。