Organ Specific Project

器官特定项目

基本信息

批准号：
10685591
负责人：
CLAYTON E MATHEWS
金额：
$ 158.75万
依托单位：
BATTELLE PACIFIC NORTHWEST LABORATORIES
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-09-10 至 2024-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10685591
关键词：
3-Dimensional Actins Address African American Age Age Years Amylases Anatomy Antibodies Antigen-Presenting Cells Arteries Atlases Bioinformatics Biology Biomedical Research Catalogs Caucasians Cd68 Cell Nucleus Cells Collaborations Collagen Collection Cytometry Data Data Analyses Diabetes Mellitus Disease Drug or chemical Tissue Distribution Duct (organ) structure Equipment and supply inventories Ethnic Origin Female Fibrosis Florida Future Genetic Goals Guidelines Head of pancreas Hispanic Human Human BioMolecular Atlas Program Image Incidence Information Distribution Infrastructure Knowledge Link Lipids Location Lymphocyte Malignant neoplasm of pancreas Maps Mass Spectrum Analysis Measurement Metabolism Metadata Molecular Molecular Profiling NCAM1 gene Nerve Online Systems Organ Organ Procurements Outcome PECAM1 gene PTPRC gene Pancreas Pancreatic Diseases Pancreatitis Peroxidases Physiology Process Production Proteins RNA Race Regulation Research Resolution Sampling Sex Bias Slide Smooth Muscle Specimen Stains Structure Synaptophysin System Tail Technology Texas Thick Tissue and Organ Procurement Tissues Transplantation United States National Institutes of Health Universities Vascularization Work fluorescence imaging improved insight islet male multiple omics nerve supply neutrophil preservation prognostic racial population reconstruction sex tissue mapping tissue processing tumor tumorigenesis type I and type II diabetes whole slide imaging

项目摘要

PROJECT ABSTRACT The overarching goal of the Human BioMolecular Atlas Program (HuBMAP) and specifically of Tissue Mapping Centers is to generate high resolution 3D biomolecular maps for non-diseased human organs. The overall objective of this pancreas organ specific project (OSP) is to identify and collect whole disease-free pancreas from donors across different sexes and ethnicities, to develop a robust system for tissue processing and distribution, and to generate multi-scale, high resolution, 3D multi-omics molecular atlases of functional regions of human pancreas. This OSP integrates unique expertise ranging from pancreas biology, diabetes, organ procurement, tissue processing, deep-tissue fluorescence imaging analysis (University of Florida), advanced multi-omics mass spectrometry-based tissue mapping (PNNL), and sophisticated high-throughput bioinformatics and 3D reconstruction of molecular maps (Texas Advanced Computing Center) of regions of pancreas, such as islets. Specifically, we will: (1) Identify and collect whole disease-free pancreas from donors through collaboration with the Organ Procurement Organizations (OPO) and Research Intermediaries (RI), and develop infrastructure for timely referral and donor collection, as well as a robust, web-based, specimen inventory and request system that accesses a catalogue of representative sample derivative meta-data (i.e. whole slide image or cytometry) of quality and composition; (2) Provide preserved, embedded, and sectioned pancreas tissue using pancreatic processing processes that have carefully mapped the pancreas in 2D; (3) Perform multi-scale and multi-omic imaging or spatially resolved characterization of pancreas and function-guided tissue sections through the characterization pipeline; and (4) Create comprehensive 3-D biomolecular atlas of pancreas that maps protein, metabolite, and lipid expression throughout functional regions of the pancreas through the Data Analysis Core.

项目摘要人类生物分子图谱计划 (HuBMAP) 的总体目标，特别是组织绘图 Centers 的目标是为未患病的人体器官生成高分辨率 3D 生物分子图。整体该胰腺器官特定项目 (OSP) 的目标是识别和收集完整的无病胰腺来自不同性别和种族的捐赠者，开发一个强大的组织处理系统分布，并生成功能的多尺度、高分辨率、3D 多组学分子图谱人类胰腺的区域。该 OSP 整合了胰腺生物学、糖尿病、器官获取、组织等独特的专业知识处理、深层组织荧光成像分析（佛罗里达大学）、先进的多组学质量基于光谱测定的组织图谱 (PNNL) 以及复杂的高通量生物信息学和 3D 重建胰腺区域（例如胰岛）的分子图谱（德克萨斯高级计算中心）。具体来说，我们将：（1）通过合作识别并收集捐赠者的完整无病胰腺与器官获取组织 (OPO) 和研究中介机构 (RI) 合作，并制定用于及时转诊和捐赠者收集的基础设施，以及强大的、基于网络的样本库存和访问代表性样本派生元数据目录（即整个幻灯片图像）的请求系统或细胞计数法）的质量和成分； (2) 提供保存、包埋、切片的胰腺组织使用胰腺处理过程仔细绘制二维胰腺图； (3) 进行多尺度胰腺和功能引导组织切片的多组学成像或空间分辨表征通过表征管道； (4) 创建全面的胰腺 3D 生物分子图谱通过数据绘制整个胰腺功能区域的蛋白质、代谢物和脂质表达图分析核心。