Organ Specific Project

器官特定项目

基本信息

批准号：
10259784
负责人：
CLAYTON E MATHEWS
金额：
$ 70.21万
依托单位：
BATTELLE PACIFIC NORTHWEST LABORATORIES
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-09-10 至 2024-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10259784
关键词：
3-Dimensional Address African American Age Age-Years Amylases Anatomy Antibodies Antigen-Presenting Cells Arteries Atlases Bioinformatics Biology Biomedical Research Catalogs Caucasians Cell Nucleus Cells Collaborations Collagen Collection Cytometry Data Data Analyses Diabetes Mellitus Disease Drug or chemical Tissue Distribution Duct (organ) structure Ensure Equipment and supply inventories Ethnic Origin Female Fibrosis Florida Future Genetic Goals Guidelines Head of pancreas Hispanics Human Human BioMolecular Atlas Program Image Incidence Information Distribution Infrastructure Insulin-Dependent Diabetes Mellitus Knowledge Link Lipids Location Lymphocyte Malignant neoplasm of pancreas Maps Mass Spectrum Analysis Measurement Metabolism Metadata Molecular Molecular Profiling NCAM1 gene Nerve Non-Insulin-Dependent Diabetes Mellitus Online Systems Organ Organ Procurements Outcome PECAM1 gene PTPRC gene Pancreas Pancreatic Diseases Pancreatitis Peroxidases Physiology Process Production Proteins RNA Race Regulation Research Resolution Sampling Sex Bias Slide Smooth Muscle Actin Staining Method Specimen Stains Structure Synaptophysin System Tail Technology Texas Thick Time Tissues Transplantation United States National Institutes of Health Universities Vascularization Work base fluorescence imaging insight islet male multiple omics nerve supply neutrophil preservation prognostic protein metabolite reconstruction sex tissue processing tumor tumorigenesis whole slide imaging

项目摘要

PROJECT ABSTRACT The overarching goal of the Human BioMolecular Atlas Program (HuBMAP) and specifically of Tissue Mapping Centers is to generate high resolution 3D biomolecular maps for non-diseased human organs. The overall objective of this pancreas organ specific project (OSP) is to identify and collect whole disease-free pancreas from donors across different sexes and ethnicities, to develop a robust system for tissue processing and distribution, and to generate multi-scale, high resolution, 3D multi-omics molecular atlases of functional regions of human pancreas. This OSP integrates unique expertise ranging from pancreas biology, diabetes, organ procurement, tissue processing, deep-tissue fluorescence imaging analysis (University of Florida), advanced multi-omics mass spectrometry-based tissue mapping (PNNL), and sophisticated high-throughput bioinformatics and 3D reconstruction of molecular maps (Texas Advanced Computing Center) of regions of pancreas, such as islets. Specifically, we will: (1) Identify and collect whole disease-free pancreas from donors through collaboration with the Organ Procurement Organizations (OPO) and Research Intermediaries (RI), and develop infrastructure for timely referral and donor collection, as well as a robust, web-based, specimen inventory and request system that accesses a catalogue of representative sample derivative meta-data (i.e. whole slide image or cytometry) of quality and composition; (2) Provide preserved, embedded, and sectioned pancreas tissue using pancreatic processing processes that have carefully mapped the pancreas in 2D; (3) Perform multi-scale and multi-omic imaging or spatially resolved characterization of pancreas and function-guided tissue sections through the characterization pipeline; and (4) Create comprehensive 3-D biomolecular atlas of pancreas that maps protein, metabolite, and lipid expression throughout functional regions of the pancreas through the Data Analysis Core.

项目摘要人类生物分子图书馆计划（Hubmap），特别是组织映射的总体目标中心将为未切除的人体器官生成高分辨率3D生物分子图。总体该胰腺特定项目（OSP）的目的是识别和收集全部无病胰腺从跨不同性别和种族的捐助者，开发一个可用于组织加工和分布，并生成功能性的多尺度，高分辨率，3D多矩分子图谱人类胰腺地区。该OSP整合了独特的专业知识，包括胰腺生物学，糖尿病，器官采购，组织加工，深部组织荧光成像分析（佛罗里达大学），高级多摩斯质量基于光谱的组织映射（PNNL）和复杂的高通量生物信息学和3D 胰腺等区域的分子图（得克萨斯州高级计算中心）的重建，例如胰岛。具体来说，我们将：（1）通过协作从捐助者那里识别并收集并收集全部无病的胰腺与器官采购组织（OPO）和研究中介机构（RI）一起开发及时推荐和捐助者收集的基础架构，以及强大的基于Web的标本清单和请求系统访问代表性样品衍生物元数据的目录（即整个幻灯片图像质量和成分的细胞仪）；（2）提供保留的，嵌入的和截面的胰腺组织使用胰腺加工过程，这些过程仔细地将胰腺映射到2D中；（3）执行多尺度以及胰腺和功能引导的组织的多摩尼克成像或空间分辨的表征通过表征管道；（4）创建胰腺的全面3-D生物分子图集通过数据，整个胰腺功能区域的地图蛋白，代谢产物和脂质表达分析核心。