Functional Analysis of TEM5 and TEM8

TEM5和TEM8的功能分析

• 批准号: 10702361
• 负责人: bradley d st croix
• 金额: $ 81.39万
• 依托单位: DIVISION OF BASIC SCIENCES - NCI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10702361
• 关键词: Adult; Blood; Cell surface; Endothelial Cells; Endothelium; Gene Expression Profiling; Genes; Goals; Growth; Knockout Mice; Laboratories; Mus; Neoplasms in Vascular Tissue; Physiological; Role; Therapeutic; Tumor Angiogenesis; Tumor Cell Line; angiogenesis; blood vessel development; conditional knockout; gene function; interest; tumor; tumorigenesis

Tumors are dependent upon new blood vessel formation, or angiogenesis, for expansive growth. Our prior analysis of gene expression led to the identification of several genes upregulated in endothelial cells that line tumor blood vessels, called TEMs. Two of these, TEM5 and TEM8 encode products that are of particular interest because they reside on the cell surface and may therefore be directly accessible to blood-borne therapeutics. In an attempt to understand the functional role of these genes in angiogenesis, we have generated TEM5 and TEM8 knockout mice. By challenging the gene disrupted mice with tumors, we are evaluating the importance of these genes in tumor angiogenesis. These studies are also enabling us to better understand the normal physiological function of these genes particularly as it relates to angiogenesis.

肿瘤的扩张生长依赖于新血管形成或血管生成。我们之前对基因表达的分析发现了肿瘤血管内皮细胞（称为 TEM）中几个上调的基因。其中两个，TEM5 和 TEM8 编码的产物特别令人感兴趣，因为它们驻留在细胞表面，因此可以直接用于血源性治疗。为了了解这些基因在血管生成中的功能作用，我们培育了 TEM5 和 TEM8 敲除小鼠。通过用肿瘤挑战基因破坏的小鼠，我们正在评估这些基因在肿瘤血管生成中的重要性。这些研究还使我们能够更好地了解这些基因的正常生理功能，特别是与血管生成相关的功能。