Diabetes Genomics and Analysis Core

糖尿病基因组学和分析核心

• 批准号: 10669005
• 负责人: HASSAN CHAIB
• 金额: $ 17.9万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-15 至 2027-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10669005
• 关键词: ATAC-seq; Abbreviations; Adoption; Architecture; Award; Bioinformatics; Biological; Biological Assay; Cell Nucleus; ChIP-seq; Chromatin; Chromatin Structure; Consultations; DNA methylation profiling; Data; Data Analyses; Development; Diabetes Mellitus; Encapsulated; Equipment; Evolution; Gene Expression Profiling; Genetic; Genome; Genomic DNA; Genomic medicine; Genomics; Goals; Growth; Health; High-Throughput Nucleotide Sequencing; High-Throughput RNA Sequencing; Hour; Human; Human Microbiome; Individual; Informatics; Insulin-Dependent Diabetes Mellitus; Laboratories; Libraries; Modernization; Non-Insulin-Dependent Diabetes Mellitus; Nucleic Acids; Nucleotides; Preparation; Regulatory Element; Research; Research Design; Research Personnel; Resources; Services; Techniques; Technology; Training; Transposase; Universities; analytical method; applied biomedical research; chromatin immunoprecipitation; computerized tools; cost; cost effective; design; experimental study; genome sequencing; human disease; innovation; islet; medical schools; member; new technology; next generation sequencing; personalized medicine; portability; programs; single cell sequencing; single-cell RNA sequencing; tool; transcriptome sequencing

DIABETES GENOMICS AND ANALYSIS CORE (DGAC): PROJECT SUMMARY/ABSTRACT The SDRC Diabetes Genomics and Analysis Core (DGAC) supports the genomic research of SDRC investigators through access to next-generation sequencing and modern bioinformatics analysis. Nucleotide sequencing has become essential for formulating and resolving crucial biological questions in many contexts, like diabetes. This includes determining the underlying causes of human diseases to monitoring gene expression, elucidating regulatory networks and exploring organismal diversity that may impact human health, like human microbiomes. Researchers use this technology increasingly for an ever-broadening range of applications to detect and characterize nucleic acid molecules (RNA and DNA). The overall goal of the DGAC is to provide SDRC investigators with a next-generation sequencing facility composed of state-of-the-art laboratory, computational facilities and engaged bio-informatics support. The DGAC is committed to providing SDRC laboratories with the expertise and technical support necessary to realize the potential of next- generation sequencing in a cost-effective manner regardless of their existing expertise in genomics. This includes active evolution of core services, like the development of the wildly popular bio-informatics ‘Office Hours’ and consulting services: thus, the DGAC helps SDRC members from ‘start to finish’ with their genomics-based experiments. We will continue to offer a complete package of services ranging from study design consultation, library preparation services, access to sequencing technologies, and analysis and interpretation of sequencing data. SDRC laboratories can leverage the technologies and expertise of the DGAC to overcome cost-prohibitive barriers of entry into genomics. Based on the strengths of the DGAC and its contributions, the specific aims are to provide SDRC core users guidance with design of their next- generation sequencing projects, access to a broad range of nucleotide library preparation services, access to sequencing services with modern sequencing technologies, and support with data analysis and interpretation of their sequencing projects. The proven strengths of the DGAC in these techniques will continue to provide investigators with a unique opportunity to effectively apply powerful research approaches that would be prohibitively difficult for individual laboratories to develop. The DGAC serves as an invaluable resource for the SDRC, providing investigators with the next-generation sequencing expertise and training necessary to examine the genome at a level of access and subsidized cost for SDRC members that is not achievable at Stanford without this core. The DGAC will also serve SDRC members at UC Berkeley and UC Davis, including those supported through the proposed Regional Pilot & Feasibility Award expansion. Based on growth of the SDRC membership, evolution of exciting new technologies, and increasing membership demand for bio- informatic support and analysis, we anticipate growth in use of the specialized and unique DGAC services in the coming years.

糖尿病基因组学和分析核心（DGAC）：项目摘要/摘要 SDRC糖尿病基因组学和分析核心（DGAC）支持SDRC的基因组研究 通过获取下一代测序和现代生物信息学分析的研究人员。核苷酸 在许多情况下，测序对于在许多情况下制定和解决关键的生物学问题至关重要， 喜欢糖尿病。这包括确定人类疾病监测基因的根本原因 表达，阐明监管网络并探索可能影响人类健康的有机多样性， 像人类微生物。研究人员越来越多地使用这项技术 检测和表征核酸分子（RNA和DNA）的应用。 DGAC的总体目标 是为SDRC调查人员提供由最先进的下一代测序设施 实验室，计算设施和敬业的生物信息学支持。 DGAC致力于提供 SDRC实验室具有实现下一步潜力所必需的专业知识和技术支持 无论其现有基因组学专业知识如何，以具有成本效益的方式进行生成测序。这 包括核心服务的积极演变，例如开发广受欢迎的生物信息学的办公室 小时和咨询服务：因此，DGAC帮助SDRC成员从“开始到结束”。 基于基因组学的实验。我们将继续提供一套完整的服务，包括学习 设计咨询，图书馆准备服务，访问测序技术以及分析以及 测序数据的解释。 SDRC实验室可以利用 DGAC以克服基因组学入口成本障碍的障碍。基于DGAC的优势和 它的贡献是，具体的目的是通过设计其下一步的SDRC核心用户指导 生成测序项目，访问广泛的核苷酸图书馆准备服务，访问 通过现代测序技术进行测序服务，并通过数据分析和解释支持 他们的测序项目。这些技术中DGAC的可靠优势将继续提供 具有独特机会的研究人员有效地采用强大的研究方法 个人实验室很难发展。 DGAC充当了无价的资源 SDRC，为调查人员提供了必要的下一代测序专业知识和培训 检查SDRC成员的访问水平和补贴成本的基因组，这是无法实现的 没有这个核心的斯坦福。 DGAC还将为UC Berkeley和UC Davis的SDRC成员提供服务，包括 那些通过拟议的区域飞行员和可行性奖扩展的支持。基于 SDRC成员资格，令人兴奋的新技术的发展以及对生物的成员需求的增加 信息丰富的支持和分析，我们预计使用专业和独特的DGAC服务的增长 未来几年。