Diabetes Genomics and Analysis Core

糖尿病基因组学和分析核心

• 批准号: 10669005
• 负责人: HASSAN CHAIB
• 金额: $ 17.9万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-15 至 2027-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10669005
• 关键词: ATAC-seq; Abbreviations; Adoption; Architecture; Award; Bioinformatics; Biological; Biological Assay; Cell Nucleus; ChIP-seq; Chromatin; Chromatin Structure; Consultations; DNA methylation profiling; Data; Data Analyses; Development; Diabetes Mellitus; Encapsulated; Equipment; Evolution; Gene Expression Profiling; Genetic; Genome; Genomic DNA; Genomic medicine; Genomics; Goals; Growth; Health; High-Throughput Nucleotide Sequencing; High-Throughput RNA Sequencing; Hour; Human; Human Microbiome; Individual; Informatics; Insulin-Dependent Diabetes Mellitus; Laboratories; Libraries; Modernization; Non-Insulin-Dependent Diabetes Mellitus; Nucleic Acids; Nucleotides; Preparation; Regulatory Element; Research; Research Design; Research Personnel; Resources; Services; Techniques; Technology; Training; Transposase; Universities; analytical method; applied biomedical research; chromatin immunoprecipitation; computerized tools; cost; cost effective; design; experimental study; genome sequencing; human disease; innovation; islet; medical schools; member; new technology; next generation sequencing; personalized medicine; portability; programs; single cell sequencing; single-cell RNA sequencing; tool; transcriptome sequencing

DIABETES GENOMICS AND ANALYSIS CORE (DGAC): PROJECT SUMMARY/ABSTRACT The SDRC Diabetes Genomics and Analysis Core (DGAC) supports the genomic research of SDRC investigators through access to next-generation sequencing and modern bioinformatics analysis. Nucleotide sequencing has become essential for formulating and resolving crucial biological questions in many contexts, like diabetes. This includes determining the underlying causes of human diseases to monitoring gene expression, elucidating regulatory networks and exploring organismal diversity that may impact human health, like human microbiomes. Researchers use this technology increasingly for an ever-broadening range of applications to detect and characterize nucleic acid molecules (RNA and DNA). The overall goal of the DGAC is to provide SDRC investigators with a next-generation sequencing facility composed of state-of-the-art laboratory, computational facilities and engaged bio-informatics support. The DGAC is committed to providing SDRC laboratories with the expertise and technical support necessary to realize the potential of next- generation sequencing in a cost-effective manner regardless of their existing expertise in genomics. This includes active evolution of core services, like the development of the wildly popular bio-informatics ‘Office Hours’ and consulting services: thus, the DGAC helps SDRC members from ‘start to finish’ with their genomics-based experiments. We will continue to offer a complete package of services ranging from study design consultation, library preparation services, access to sequencing technologies, and analysis and interpretation of sequencing data. SDRC laboratories can leverage the technologies and expertise of the DGAC to overcome cost-prohibitive barriers of entry into genomics. Based on the strengths of the DGAC and its contributions, the specific aims are to provide SDRC core users guidance with design of their next- generation sequencing projects, access to a broad range of nucleotide library preparation services, access to sequencing services with modern sequencing technologies, and support with data analysis and interpretation of their sequencing projects. The proven strengths of the DGAC in these techniques will continue to provide investigators with a unique opportunity to effectively apply powerful research approaches that would be prohibitively difficult for individual laboratories to develop. The DGAC serves as an invaluable resource for the SDRC, providing investigators with the next-generation sequencing expertise and training necessary to examine the genome at a level of access and subsidized cost for SDRC members that is not achievable at Stanford without this core. The DGAC will also serve SDRC members at UC Berkeley and UC Davis, including those supported through the proposed Regional Pilot & Feasibility Award expansion. Based on growth of the SDRC membership, evolution of exciting new technologies, and increasing membership demand for bio- informatic support and analysis, we anticipate growth in use of the specialized and unique DGAC services in the coming years.

糖尿病基因组学和分析核心 (DGAC)：项目摘要/摘要 SDRC 糖尿病基因组学和分析核心 (DGAC) 支持 SDRC 的基因组研究 研究人员通过新一代测序和现代生物信息学分析。 测序对于在许多情况下制定和解决关键生物学问题至关重要， 例如糖尿病，这包括确定人类疾病的根本原因以监测基因。 表达，阐明监管网络并探索可能影响人类健康的生物多样性， 研究人员越来越多地将这项技术用于越来越广泛的范围。 检测和表征核酸分子（RNA 和 DNA）的应用 DGAC 的总体目标。 旨在为 SDRC 研究人员提供由最先进的技术组成的下一代测序设施 DGAC 致力于提供实验室、计算设施和生物信息学支持。 SDRC 实验室拥有实现下一代潜力所需的专业知识和技术支持 无论他们现有的基因组学专业知识如何，都能以经济有效的方式进行世代测序。 包括核心服务的积极发展，例如广受欢迎的生物信息学“Office”的开发 时间”和咨询服务：因此，DGAC 帮助 SDRC 成员从“开始到结束” 我们将继续提供从研究在内的一整套服务。 设计咨询、文库制备服务、测序技术的获取以及分析和 SDRC 实验室可以利用测序数据的技术和专业知识。 DGAC 克服了进入基因组学领域的成本高昂的障碍。 其贡献，具体目标是为 SDRC 核心用户提供下一步设计的指导 一代测序项目、获得广泛的核苷酸文库制备服务、获得 采用现代测序技术的测序服务，并提供数据分析和解释支持 DGAC 在这些技术方面的成熟优势将继续提供。 研究人员有独特的机会有效地应用强大的研究方法 DGAC 对于单个实验室来说是非常宝贵的资源。 SDRC，为研究人员提供下一代测序专业知识和必要的培训 以 SDRC 成员的访问水平和补贴成本检查基因组，这是目前无法实现的 没有这个核心的斯坦福大学 DGAC 还将为加州大学伯克利分校和加州大学戴维斯分校的 SDRC 成员提供服务，包括 那些通过拟议的区域试点和可行性奖励扩展来支持的项目 基于增长。 SDRC 会员资格、令人兴奋的新技术的发展以及对生物技术日益增长的会员需求 信息支持和分析，我们预计专业且独特的 DGAC 服务的使用将会增长 未来几年。