Diabetes Genomics and Analysis Core

糖尿病基因组学和分析核心

• 批准号: 10197907
• 负责人: HASSAN CHAIB
• 金额: $ 21.19万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-15 至 2022-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10197907
• 关键词: ATAC-seq; Abbreviations; Bioinformatics; Biological; Biological Assay; ChIP-seq; Chromatin; Consultations; DNA methylation profiling; Data; Data Analyses; Diabetes Mellitus; Equipment; Gene Expression Profiling; Genetic; Genome; Genomic DNA; Genomic medicine; Genomics; Goals; Health; High-Throughput Nucleotide Sequencing; High-Throughput RNA Sequencing; Human; Human Microbiome; Individual; Informatics; Insulin-Dependent Diabetes Mellitus; Laboratories; Libraries; Modernization; Non-Insulin-Dependent Diabetes Mellitus; Nucleic Acids; Nucleotides; Preparation; Research; Research Design; Research Personnel; Resources; Services; Techniques; Technology; Training; Universities; applied biomedical research; base; chromatin immunoprecipitation; computer infrastructure; cost; cost effective; design; experimental study; genome sequencing; human disease; islet; medical schools; next generation sequencing; personalized medicine; programs; tool; transcriptome sequencing

Project Summary / Abstract (DGA Core) The Stanford Diabetes Genomics and Analysis (DGA) Core is designed to support the genomic research of Stanford Diabetes Research Center (SDRC) investigators through access to next-generation sequencing and modern bioinformatics analysis. Nucleotide sequencing has become essential for formulating and resolving crucial biological questions in many contexts, like diabetes. This includes determining the underlying causes of human diseases to monitoring gene expression, elucidating regulatory networks and exploring organismal diversity that may impact human health, like human microbiomes. Thus, high-throughput sequencers have become a transformative tool for a wide variety of studies in both pure and applied biomedical science. Researchers use this technology increasingly for an ever-broadening range of applications to detect and characterize nucleic acid molecules (RNA and DNA). The overall goal of the Stanford DGA Core is to provide SDRC investigators with a next-generation sequencing facility composed of state-of-the-art laboratory, computational facilities and informatics support. The DGA Core is committed to providing SDRC laboratories with the expertise and technical support necessary to realize the potential of next-generation sequencing in a cost-effective matter regardless of their existing expertise in genomics. The Stanford DGA Core will offer a complete package of services ranging from study design consultation, library preparation services, access to sequencing technologies, and analysis and interpretation of sequencing data. SDRC laboratories can leverage the technologies and expertise of the Stanford DGA Core to overcome cost-prohibitive barriers of entry into genomics which include technologically advanced facilities and equipment, and computational infrastructure that can only be achieved in a center setting like the Stanford DGA Core. Based on the strengths of the Stanford DGA Core and its contributions, the specific aims of the DGA Core are to provide SDR core users guidance with design of their next-generation sequencing projects, access to a broad range of nucleotide library preparation services, access to sequencing services with modern sequencing technologies, and support with data analysis and interpretation of their sequencing projects The proven strengths of the Stanford DGA Core in these techniques will provide investigators with a unique opportunity to effectively apply powerful research approaches that would be prohibitively difficult for individual laboratories to develop. The Stanford DGA Core will serve as an invaluable resource for the SDRC, providing investigators with the next-generation sequencing expertise and training necessary to examine the genome at a level that is limited to a relatively few laboratories. This Core is committed to serving SDRC investigators in their experiments, from start to finish.

项目摘要/摘要（DGA 核心） 斯坦福大学糖尿病基因组学和分析 (DGA) 核心旨在支持以下疾病的基因组研究： 斯坦福糖尿病研究中心 (SDRC) 研究人员通过获取下一代测序和 现代生物信息学分析。核苷酸测序对于制定和解决问题至关重要 在许多情况下，例如糖尿病，至关重要的生物学问题。这包括确定问题的根本原因 人类疾病监测基因表达、阐明调控网络和探索有机体 可能影响人类健康的多样性，例如人类微生物组。因此，高通量测序仪具有 成为纯粹和应用生物医学领域各种研究的变革性工具。 研究人员越来越多地将这项技术用于不断扩大的应用范围，以检测和 表征核酸分子（RNA 和 DNA）。斯坦福 DGA 核心的总体目标是提供 SDRC 研究人员拥有由最先进的实验室组成的下一代测序设施， 计算设施和信息学支持。 DGA 核心致力于为 SDRC 实验室提供专业知识和技术支持 以具有成本效益的方式实现下一代测序的潜力是必要的，无论它们如何 现有的基因组学专业知识。斯坦福 DGA 核心将提供一整套服务，包括 研究设计咨询、文库制备服务、测序技术的获取以及分析和 测序数据的解释。 SDRC 实验室可以利用 斯坦福 DGA 核心将克服进入基因组学领域的成本高昂的障碍，其中包括技术 先进的设施设备和计算基础设施只有在中心才能实现 设置类似于斯坦福 DGA 核心。基于斯坦福 DGA 核心的优势及其贡献， DGA 核心的具体目标是为 SDR 核心用户提供下一代设计指导 测序项目、获得广泛的核苷酸文库制备服务、获得测序 采用现代测序技术的服务，并提供数据分析和解释的支持 测序项目 斯坦福 DGA 核心在这些技术中经过验证的优势将为研究人员提供独特的 有机会有效地应用强大的研究方法，而这对于个人来说是极其困难的 实验室来开发。斯坦福 DGA 核心将成为 SDRC 的宝贵资源，提供 具有下一代测序专业知识和检查基因组所需培训的研究人员 仅限于相对较少的实验室的水平。该核心致力于为 SDRC 调查人员提供服务 他们的实验，从开始到结束。