Diabetes Genomics and Analysis Core

糖尿病基因组学和分析核心

• 批准号: 9980404
• 负责人: HASSAN CHAIB
• 金额: $ 21.19万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9980404
• 关键词: ATAC-seq; Abbreviations; Bioinformatics; Biological; Biological Assay; ChIP-seq; Chromatin; Consultations; Data; Data Analyses; Diabetes Mellitus; Equipment; Gene Expression Profiling; Genetic; Genome; Genomic DNA; Genomic medicine; Genomics; Goals; Health; High-Throughput Nucleotide Sequencing; High-Throughput RNA Sequencing; Human; Human Microbiome; Individual; Informatics; Insulin-Dependent Diabetes Mellitus; Laboratories; Libraries; Methylation; Modernization; Non-Insulin-Dependent Diabetes Mellitus; Nucleic Acids; Nucleotides; Preparation; Research; Research Design; Research Personnel; Resources; Services; Techniques; Technology; Training; Universities; applied biomedical research; base; chromatin immunoprecipitation; computer infrastructure; cost; cost effective; design; experimental study; genome sequencing; human disease; islet; medical schools; next generation sequencing; personalized medicine; programs; tool; transcriptome sequencing

Project Summary / Abstract (DGA Core) The Stanford Diabetes Genomics and Analysis (DGA) Core is designed to support the genomic research of Stanford Diabetes Research Center (SDRC) investigators through access to next-generation sequencing and modern bioinformatics analysis. Nucleotide sequencing has become essential for formulating and resolving crucial biological questions in many contexts, like diabetes. This includes determining the underlying causes of human diseases to monitoring gene expression, elucidating regulatory networks and exploring organismal diversity that may impact human health, like human microbiomes. Thus, high-throughput sequencers have become a transformative tool for a wide variety of studies in both pure and applied biomedical science. Researchers use this technology increasingly for an ever-broadening range of applications to detect and characterize nucleic acid molecules (RNA and DNA). The overall goal of the Stanford DGA Core is to provide SDRC investigators with a next-generation sequencing facility composed of state-of-the-art laboratory, computational facilities and informatics support. The DGA Core is committed to providing SDRC laboratories with the expertise and technical support necessary to realize the potential of next-generation sequencing in a cost-effective matter regardless of their existing expertise in genomics. The Stanford DGA Core will offer a complete package of services ranging from study design consultation, library preparation services, access to sequencing technologies, and analysis and interpretation of sequencing data. SDRC laboratories can leverage the technologies and expertise of the Stanford DGA Core to overcome cost-prohibitive barriers of entry into genomics which include technologically advanced facilities and equipment, and computational infrastructure that can only be achieved in a center setting like the Stanford DGA Core. Based on the strengths of the Stanford DGA Core and its contributions, the specific aims of the DGA Core are to provide SDR core users guidance with design of their next-generation sequencing projects, access to a broad range of nucleotide library preparation services, access to sequencing services with modern sequencing technologies, and support with data analysis and interpretation of their sequencing projects The proven strengths of the Stanford DGA Core in these techniques will provide investigators with a unique opportunity to effectively apply powerful research approaches that would be prohibitively difficult for individual laboratories to develop. The Stanford DGA Core will serve as an invaluable resource for the SDRC, providing investigators with the next-generation sequencing expertise and training necessary to examine the genome at a level that is limited to a relatively few laboratories. This Core is committed to serving SDRC investigators in their experiments, from start to finish.

项目摘要 /摘要（DGA核心） 斯坦福糖尿病基因组学和分析（DGA）核心旨在支持 斯坦福糖尿病研究中心（SDRC）调查人员通过获得下一代测序和 现代生物信息学分析。核苷酸测序对于制定和分辨已经至关重要 在许多情况下，例如糖尿病，关键的生物学问题。这包括确定 人类疾病以监测基因表达，阐明调节网络并探索有机体 可能影响人类健康的多样性，例如人类微生物组。因此，高通量序列仪具有 成为纯生物医学科学和应用生物医学科学的各种研究的变革性工具。 研究人员越来越多地使用该技术来检测和 表征核酸分子（RNA和DNA）。斯坦福DGA核心的总体目标是提供 SDRC研究人员的下一代测序设施由最先进的实验室组成， 计算设施和信息学支持。 DGA核心致力于为SDRC实验室提供专业知识和技术支持 在具有成本效益的问题中实现下一代测序的潜力所必需的必要 现有基因组学专业知识。斯坦福DGA核心将提供一套完整的服务，从 研究设计咨询，图书馆准备服务，访问测序技术以及分析以及 测序数据的解释。 SDRC实验室可以利用 斯坦福DGA核心要克服基因组的入境成本障碍，其中包括技术 高级设施和设备以及只能在中心实现的计算基础设施 像斯坦福DGA核心一样设置。根据斯坦福DGA核心的优势及其贡献， DGA核心的具体目标是为SDR Core用户提供下一代的设计 测序项目，访问广泛的核苷酸库准备服务，访问测序 使用现代测序技术的服务，并支持数据分析和解释其 测序项目 斯坦福DGA核心在这些技术中的经过验证的优势将为调查人员提供独特的 有效地采用强大的研究方法的机会，这对于个人来说是极难的 开发实验室。斯坦福DGA核心将成为SDRC的宝贵资源 具有下一代测序专业知识和培训的研究人员，以检查基因组 一个仅限于相对较少的实验室的水平。该核心致力于为SDRC调查人员提供服务 他们的实验从头到尾。