Conventional 200 keV Transmission Electron Microscope

传统 200 keV 透射电子显微镜

基本信息

批准号：
10632798
负责人：
WALTER G JEROME
金额：
$ 59.7万
依托单位：
VANDERBILT UNIVERSITY MEDICAL CENTER
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-04-01 至 2025-03-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10632798
关键词：
20 year old Academic Medical Centers Acceleration Age Area Automation Businesses Cancer Biology Cell surface Cells Cellular Membrane Communities Computer software Congenital Abnormality Data Development Digestive System Disorders Disease Electron Microscope Epithelium Extracellular Matrix Filament Funding Grant Health Human Image Infrastructure Investigation Maintenance Malignant Neoplasms Microscope Mitochondria Modeling Modernization Molecular Motor Pattern Recording of previous events Request for Proposals Research Research Personnel Research Project Grants Resolution Resource Sharing Sampling System Techniques Thick Tomogram Training United States National Institutes of Health Universities Vacuum cell assembly cellular imaging extracellular vesicles flexibility imaging capabilities instrument metabolic phenotype operation tomography trafficking transmission process voltage

项目摘要

PROJECT SUMMARY This proposal requests funds to acquire a transmission electron microscope (TEM) to support a diverse group of NIH-funded research projects at Vanderbilt University Medical Center and Vanderbilt University. The JEOL JEM 2100 Plus TEM identified in this grant is a modern 200 keV thermionic TEM. The TEM is equipped with a large format CMOS camera, automatic motorized apertures, turbo molecular vacuum system, and full integration SerialEM automation software. This TEM will replace the Cell Imaging Shared Resource’s (CISR) 20-year-old ThermoScientific Tecnai T12, a 120 keV LaB6 filament microscope. The Tecnai T12 has served the Vanderbilt community well throughout its lifetime facilitating numerous grants and studies, but its age has become an impediment to research progress with increasingly large downtimes, scarce availability of parts, and lack of modern features. Notably, this current Tecnai T12 TEM has been maintained by the CISR for almost its entire 20-year history. The 2100 Plus will not only serve as a suitable replacement for the Tecnai T12 but also add new capabilities identified as important to the Major User group. The 2100 Plus has tremendous flexibility, supporting accelerating voltages between 80-200 keV, allowing optimum imaging for small samples such as extracellular vesicles at lower voltage and up to 200 keV to image relatively thick sections for tomography. Acquiring high- quality tomograms of volumetric data will provide critical new capabilities increasingly required for state-of-the art analysis. The 2100 Plus’s stage, large format camera, and SerialEM automation software will also allow for efficient tiling of large areas at high-resolution; this type of data is becoming paramount for data quantification. Our Major Users will use the JEOL 2100 Plus to facilitate a wide range of NIH-funded research projects in cell, developmental, epithelial, and cancer biology. Projects include assembly of cell surface features (Goldenring, Tyska), metabolic phenotypes of mitochondria (Gannon, Rathmell, Skaar), extracellular matrix patterning (Kuchtey, Weaver), and cellular membrane trafficking (Weaver, Goldenring, Gannon). Together these investigations hold significance for our understanding of disease and illness related to birth defects, cancer, digestive disorders and many other human health problems. CISR will provide the infrastructure for introducing researchers across Vanderbilt to the newly available techniques, train users in operation of the instrument, and apply an established business model to support the long-term operation and maintenance of the system. In summary, acquisition of the JEOL 2100 Plus will allow for imaging capabilities at scales and spatial resolutions not currently available to the Vanderbilt community and will facilitate research on a diverse set of topics.

项目概要该提案要求资金购买透射电子显微镜 (TEM) 以支持多元化群体范德比尔特大学医学中心和 JEOL 的 NIH 资助研究项目。本次资助中确定的 JEM 2100 Plus TEM 是一款现代化的 200 keV 热离子 TEM。大画幅 CMOS 相机、自动电动光圈、涡轮分子真空系统、全集成 SerialEM 自动化软件将取代已有 20 年历史的细胞成像共享资源 (CISR)。 ThermoScientific Tecnai T12，一款 120 keV LaB6 灯丝显微镜 Tecnai T12 已服务于范德比尔特大学。社区在其一生中促进了大量的资助和研究，但它的年龄已经成为一个停机时间越来越长、零件稀缺以及缺乏值得注意的是，当前的 Tecnai T12 TEM 几乎自始至终都由 CISR 维护。 20 年历史。2100 Plus 不仅可以作为 Tecnai T12 的合适替代品，还可以添加新功能。 2100 Plus 具有极大的灵活性，支持。 80-200 keV 之间的加速电压，可为细胞外等小样本提供最佳成像囊泡在较低电压和高达 200 keV 下成像相对较厚的切片以进行断层扫描。体积数据的质量断层图将提供日益需要的关键新功能 2100 Plus 的舞台、大画幅相机和 SerialEM 自动化软件也将允许进行艺术分析。以高分辨率有效地平铺大面积；这种类型的数据对于数据量化变得至关重要。我们的主要用户将使用 JEOL 2100 Plus 来促进 NIH 资助的各种细胞研究项目，发育、上皮和癌症生物学项目包括细胞表面特征的组装（Goldenring、 Tyska），线粒体代谢表型（Gannon，Rathmell，Skaar），细胞外基质模式（Kuchtey，Weaver）和细胞膜运输（Weaver，Goldenring，Gannon）。调查对于我们了解与出生缺陷、癌症、 CISR 将为引入消化系统疾病和许多其他人类健康问题提供基础设施。范德比尔特大学的研究人员了解最新技术，培训用户操作仪器，以及应用既定的业务模型来支持系统的长期运营和维护。总之，购买 JEOL 2100 Plus 将实现尺度和空间分辨率的成像能力目前范德比尔特社区尚不可用，并将促进对不同主题的研究。