Conventional 200 keV Transmission Electron Microscope

传统 200 keV 透射电子显微镜

• 批准号: 10632798
• 负责人: WALTER G JEROME
• 金额: $ 59.7万
• 依托单位: VANDERBILT UNIVERSITY MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-04-01 至 2025-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10632798
• 关键词: 20 year old; Academic Medical Centers; Acceleration; Age; Area; Automation; Businesses; Cancer Biology; Cell surface; Cells; Cellular Membrane; Communities; Computer software; Congenital Abnormality; Data; Development; Digestive System Disorders; Disease; Electron Microscope; Epithelium; Extracellular Matrix; Filament; Funding; Grant; Health; Human; Image; Infrastructure; Investigation; Maintenance; Malignant Neoplasms; Microscope; Mitochondria; Modeling; Modernization; Molecular; Motor; Pattern; Recording of previous events; Request for Proposals; Research; Research Personnel; Research Project Grants; Resolution; Resource Sharing; Sampling; System; Techniques; Thick; Tomogram; Training; United States National Institutes of Health; Universities; Vacuum; cell assembly; cellular imaging; extracellular vesicles; flexibility; imaging capabilities; instrument; metabolic phenotype; operation; tomography; trafficking; transmission process; voltage

PROJECT SUMMARY This proposal requests funds to acquire a transmission electron microscope (TEM) to support a diverse group of NIH-funded research projects at Vanderbilt University Medical Center and Vanderbilt University. The JEOL JEM 2100 Plus TEM identified in this grant is a modern 200 keV thermionic TEM. The TEM is equipped with a large format CMOS camera, automatic motorized apertures, turbo molecular vacuum system, and full integration SerialEM automation software. This TEM will replace the Cell Imaging Shared Resource’s (CISR) 20-year-old ThermoScientific Tecnai T12, a 120 keV LaB6 filament microscope. The Tecnai T12 has served the Vanderbilt community well throughout its lifetime facilitating numerous grants and studies, but its age has become an impediment to research progress with increasingly large downtimes, scarce availability of parts, and lack of modern features. Notably, this current Tecnai T12 TEM has been maintained by the CISR for almost its entire 20-year history. The 2100 Plus will not only serve as a suitable replacement for the Tecnai T12 but also add new capabilities identified as important to the Major User group. The 2100 Plus has tremendous flexibility, supporting accelerating voltages between 80-200 keV, allowing optimum imaging for small samples such as extracellular vesicles at lower voltage and up to 200 keV to image relatively thick sections for tomography. Acquiring high- quality tomograms of volumetric data will provide critical new capabilities increasingly required for state-of-the art analysis. The 2100 Plus’s stage, large format camera, and SerialEM automation software will also allow for efficient tiling of large areas at high-resolution; this type of data is becoming paramount for data quantification. Our Major Users will use the JEOL 2100 Plus to facilitate a wide range of NIH-funded research projects in cell, developmental, epithelial, and cancer biology. Projects include assembly of cell surface features (Goldenring, Tyska), metabolic phenotypes of mitochondria (Gannon, Rathmell, Skaar), extracellular matrix patterning (Kuchtey, Weaver), and cellular membrane trafficking (Weaver, Goldenring, Gannon). Together these investigations hold significance for our understanding of disease and illness related to birth defects, cancer, digestive disorders and many other human health problems. CISR will provide the infrastructure for introducing researchers across Vanderbilt to the newly available techniques, train users in operation of the instrument, and apply an established business model to support the long-term operation and maintenance of the system. In summary, acquisition of the JEOL 2100 Plus will allow for imaging capabilities at scales and spatial resolutions not currently available to the Vanderbilt community and will facilitate research on a diverse set of topics.

项目摘要 该提案要求资金购买传输电子显微镜（TEM）以支持一个多元化的群体 范德比尔特大学医学中心和范德比尔特大学的NIH资助研究项目。耶尔 JEM 2100以上在这笔赠款中确定的TEM是现代的200 keV热TEM。 TEM等效于 大格式CMOS摄像头，自动电动孔，涡轮分子真空系统和完全集成 Serialem自动化软件。此TEM将取代单元成像共享资源（CISR）20岁 热科学Tecnai T12，120 KeV Lab6细丝显微镜。 Tecnai T12已为范德比尔特服务 在整个一生中，社区都很好地促进了众多赠款和研究，但其年龄已成为 妨碍研究进展的障碍，越来越大的降落，零件的可用性稀缺以及缺乏 现代功能。值得注意的是，该电流Tecnai T12 TEM已由CISR维持几乎整个 20年的历史。 2100 Plus不仅可以作为Tecnai T12的合适替代品，而且还可以添加新的 功能对主要用户组很重要。 2100 Plus具有巨大的灵活性，支持 加速电压在80-200 keV之间，可为小样品（例如细胞外样品）进行最佳成像 较低电压的蔬菜和多达200 keV，以形象相对较厚的部分进行断层扫描。获得高级 体积数据的优质断层图将为您提供越来越多的新功能的重要新功能 艺术分析。 2100 Plus的舞台，大格式摄像头和Serialem自动化软件也将允许 高分辨率的大面积有效地倾斜；这种类型的数据已成为数据数量的最重要成员。 我们的主要用户将使用JEOL 2100 Plus来促进NIH资助的细胞中广泛的研究项目， 发育，上皮和癌症生物学。项目包括组装细胞表面特征（Goldenring， Tyska），线粒体的代谢表型（Gannon，Rathmell，Skaar），细胞外基质图案 （Kuchtey，Weaver）和细胞膜贩运（Weaver，Goldenring，Gannon）。在一起 调查对我们对疾病和疾病的理解至关重要，与先天缺陷，癌症， 消化系统疾病和许多其他人类健康问题。 CISR将提供引入的基础架构 范德比尔特（Vanderbilt 应用既定的业务模型来支持系统的长期操作和维护。在 摘要，获取JEOL 2100 Plus将允许在尺度和空间分辨率下进行成像功能 目前尚不适用于范德比尔特社区，并将促进对潜水员一组主题的研究。