FEI Quanta 200 FEG

FEI 广达 200 FEG

• 批准号: 7791227
• 负责人: WALTER G JEROME
• 金额: $ 48.94万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-13 至 2011-05-12
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7791227
• 关键词: Academic Medical Centers; Biological; Biomedical Research; Cells; Cellular Morphology; Charge; Confocal Microscopy; Contract Services; Development; Electron Microscope; Engineering; Fluorescence Microscopy; Funding; Housing; Laboratories; Life; Lipids; Methods; Microscope; Microscopy; Mutate; Productivity; Proteins; Research Infrastructure; Research Personnel; Resource Sharing; Resources; Scanning; Scanning Electron Microscopy; Science; Spectrum Analysis; Technology; Time; Tissues; Training; Training and Education; Universities; biological research; cellular imaging; college; design; instrument; instrumentation; microbial; programs; quantum; transmission process; two-photon; uptake

DESCRIPTION (provided by applicant): We request funds to purchase a shared FEI Quanta 200 FEG Scanning Electron Microscope (SEM). The instrument will be used to support 5 major projects from NIH-funded investigators as well as provide support to a number of other NIH-funded programs. At present, the only SEM available at Vanderbilt is one maintained by the College of Engineering and used primarily for materials science applications. Use of this instrument has been critical for introducing the use of SEM to Vanderbilt biomedical researchers. However, the most mature projects now require an instrument that is designed and dedicated for their specific biological needs. Five Major Users with immediate, essential and appropriate need for the requested instrument will utilize 80% of total instrument activity. The projects range from analyzing changes in cell morphology resulting from mutated proteins in order to probe the function of those proteins, investigating the mechanism of microbial invasion of mammalian tissue, aiding the development of new methods of visualizing the 3D ultrastructure of cells, and investigating uptake and secretion of lipids. Another 3 projects have an immediate need for some access to SEM (see Other User List). This will utilize an additional 10% of the initial instrument usage. In addition, we know from historical precedent that placing a FEI Quanta FEG SEM dedicated to biomedical research in the established and widely used Cell Imaging Shared Resource (CISR) will also spur the use of this technology by many more NIH-sponsored Vanderbilt researchers. Instrument time for our other instrumentation is used >90% by NIH-funded projects. We expect that the new SEM will match this profile. The CISR is a core laboratory that supplies access to sophisticated microscopy. All Major Users on this application will have access to the instrument and training through the established CISR infrastructure. Usage charges have supported the service contract for the existing microscopes over the last eight years, and we foresee no difficulty in continuing that arrangement for the Quanta 200 FEG SEM. In addition, the CISR staff will identify from existing CISR users those researchers whose projects would benefit from SEM. The Resource has an extensive track record of education, training, and productivity with over 250 lab groups at Vanderbilt University. Over the last 16 years, we have introduced shared access to confocal microscopy, live cell imaging, two-photon excitation, total internal reflection (TIRF) microscopy, fluorescence correlation spectroscopy, deconvolution microscopy and transmission and scanning electron microscopy. Having access to an SEM that is specifically configured for biomedical research and housed within the Vanderbilt University Medical Center's Cell Imaging Shared Resource (CISR) will offer significant advantages for biological research at Vanderbilt and will greatly facilitate the use of SEM by NIH-funded biomedical researchers.

描述（由申请人提供）：我们请求资金购买共享的 FEI Quanta 200 FEG 扫描电子显微镜 (SEM)。该仪器将用于支持 NIH 资助的研究人员的 5 个主要项目，并为 NIH 资助的许多其他项目提供支持。目前，范德比尔特大学唯一可用的 SEM 是由工程学院维护的，主要用于材料科学应用。该仪器的使用对于向范德比尔特生物医学研究人员介绍 SEM 的使用至关重要。然而，最成熟的项目现在需要一种专为满足其特定生物学需求而设计的仪器。 对所请求仪器有直接、必要和适当需求的五个主要用户将利用仪器总活动的 80%。这些项目包括分析突变蛋白质引起的细胞形态变化以探究这些蛋白质的功能、研究微生物侵入哺乳动物组织的机制、帮助开发可视化细胞 3D 超微结构的新方法以及研究摄取和脂质的分泌。另外 3 个项目迫切需要访问 SEM（请参阅其他用户列表）。这将额外利用初始仪器使用量的 10%。此外，我们从历史先例中得知，将专门用于生物医学研究的 FEI Quanta FEG SEM 放入已建立且广泛使用的细胞成像共享资源 (CISR) 中，也将刺激更多 NIH 资助的范德比尔特研究人员使用该技术。我们其他仪器的仪器时间被 NIH 资助的项目使用超过 90%。我们预计新的 SEM 将符合这一配置。 CISR 是一个提供先进显微镜技术的核心实验室。该应用程序的所有主要用户都可以通过已建立的 CISR 基础设施访问该仪器并接受培训。在过去八年里，使用费一直支持现有显微镜的服务合同，我们预计继续为 Quanta 200 FEG SEM 提供这种安排不会有困难。此外，CISR 工作人员将从现有的 CISR 用户中识别出那些其项目将从 SEM 中受益的研究人员。该资源在范德比尔特大学拥有超过 250 个实验室小组，在教育、培训和生产力方面拥有广泛的记录。在过去的 16 年里，我们引入了共焦显微镜、活细胞成像、双光子激发、全内反射 (TIRF) 显微镜、荧光相关光谱、反卷积显微镜以及透射和扫描电子显微镜的共享访问。访问专门为生物医学研究配置并位于范德比尔特大学医学中心细胞成像共享资源 (CISR) 内的 SEM 将为范德比尔特大学的生物研究提供显着优势，并将极大地促进 NIH 资助的生物医学研究人员使用 SEM 。