Automation of Liquid Nitrogen Freezer for Cryopreservation of Unique Human Biospecimens at the Vanderbilt Biospecimen Storage Facility

在范德比尔特生物样本储存设施中使用液氮冷冻机自动化冷冻保存独特的人类生物样本

• 批准号: 10736276
• 负责人: Alexander Bick
• 金额: $ 25.28万
• 依托单位: VANDERBILT UNIVERSITY MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-08-01 至 2024-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10736276
• 关键词: Academic Medical Centers; Acceleration; Automation; Automobile Driving; Award; Basic Science; Biological; Biological Markers; Biological Sciences; COVID-19 pandemic; COVID-19 patient; COVID-19 prevention; Canada; Clinic; Clinical; Clinical Research; Clinical Sciences; Collection; Cooperative Human Tissue Network; Cryopreservation; Data; Disease; Disease Progression; Ensure; Environment; Friends; Funding; Future; Genetic; Genotype; Glass; Human; Individual; Infrastructure; Institution; International; Investigation; Investments; Link; Liquid substance; Monitor; Monoclonal Antibodies; National Cancer Institute; Nitrogen; Phenotype; Reagent; Recovery; Research Personnel; Resource Sharing; Resources; Retrieval; Risk; Robotics; SARS-CoV-2 antibody; Safety; Sampling; Services; Specimen; System; Technology; Temperature; Tissue Sample; Tissues; Transition Temperature; Translational Research; Translations; United States; United States National Institutes of Health; Vaccines; Water; Withdrawal; animal tissue; anticancer research; biobank; cancer care; cell preparation; cost; cost effective; cryogenics; disorder risk; high dimensionality; human disease; human tissue; improved; migration; personalized medicine; preservation; repository; treatment response; vaccinology

Project Summary Large, high-dimensional clinical, genetic and biomarker data linked to bio-repositories are driving basic, clinical and translational research. For the past 20 years, Vanderbilt University Medical Center (VUMC) has made long-term strategic investments in translational sciences as a platform for excellence across clinical and basic science departments and is internationally recognized as a leader in such fields as personalized medicine, vaccinology, and cancer care and research. As examples, BioVU, Vanderbilt’s large-scale biorepository, contains detailed de-identified human phenotype information linked to de-identified discarded samples and genotypes. Viably cryopreserved human specimens enabled the Vanderbilt Vaccine Center to isolate monoclonal antibodies against COVID-19 that were developed into Evusheld, a therapy to prevent COVID-19 in at-risk individuals. Cooperative Human Tissue Network at VUMC is one of only six funded by the National Cancer Institute to procure and distribute human tissues to researchers throughout the US, Canada, and internationally. These resources and others provide an accelerating engine of discovery. Collectively these endeavors are providing powerful predictors of disease risk, disease progression or response to therapy and underpinning studies delineating the fundamental mechanism of disease as well as translation to the clinic. These large-scale initiatives generate a massive number of valuable human and animal tissue samples. These tissues can only be viably cryopreserved when stored in liquid nitrogen. The size of these biorepositories is exceeding the capacity of individual investigators or groups to effectively curate, maintain sample integrity and rapidly retrieve samples for further study. Many of these materials could not be replaced and are of international importance. To meet this large and growing need, a specialized automated -190°C Cryogenic Freezer for Sample Storage system is necessary to manage, store and retrieve the tens of thousands of biorepository samples. This proposal seeks to obtain funding for the purchase of an Azenta BioStore Cryo M60 robotic liquid nitrogen freezer system that can accommodate all large biorepositories at Vanderbilt. With such a system Vanderbilt can provide the necessary institutional support, environment and expertise to ensure cost- effective, quality assured management, storage and retrieval of these critical samples and reagents. The technology is durable and scalable and therefore it can be predicted that this automated system will enable a broad range of investigators and propel basic, clinical and translational research long into the future.

项目摘要 与生物重复症相关的大型，高维临床，遗传和生物标志物数据正在推动基本，临床 并翻译研究。在过去的20年中，范德比尔特大学医学中心（VUMC）制作了 转化科学的长期战略投资，作为在临床和基本的卓越的平台 科学部门，在国际上被公认为是个性化医学等领域的领导者， 疫苗学和癌症护理和研究。例如，范德比尔特（Biovu 包含详细识别的人类表型信息，这些信息与被取消识别的废弃样品和 基因型。 Vander保存的人类标本使Vanderbilt疫苗中心隔离 针对Covid-19 在高危个人中。 VUMC的合作人组织网络是由国家资助的六个 癌症研究所，以采购和分配人类组织，向美国，加拿大和 国际。这些资源和其他资源提供了发现的加速引擎。集体这些 努力为疾病风险，疾病进展或对治疗的反应提供强大的预测指标 基础研究描述了疾病基本机制以及转化为诊所的研究。 这些大规模的举措产生了大量有价值的人类和动物组织样本。这些 只有在液氮中存储时，组织才能冷冻保存。这些生物震动的大小是 超过个人调查人员或小组有效策划，保持样本完整性和的能力 快速检索样品以进行进一步研究。这些材料中的许多无法替换，并且是 国际重要性。为了满足这一巨大的需求，专门的自动化-190°C低温 用于样品存储系统的冰柜需要管理，存储和检索数万 生物验证样品。该建议旨在获得购买Azenta Biostore Cryo M60的资金 机器人液氮冷冻系统，可以容纳范德比尔特的所有大型生物库。这样的 范德比尔特系统可以提供必要的机构支持，环境和专业知识，以确保成本 - 这些关键样品和试剂的有效，质量假定的管理，存储和检索。 技术是耐用且可扩展的，因此可以预测，该自动化系统将使 广泛的研究人员和推动基本，临床和翻译研究的研究很长一段时间。