Developing platforms for studying the impact of external stresses on multispecies biofilms.

开发用于研究外部压力对多物种生物膜影响的平台。

• 批准号: 10650399
• 负责人: Akhenaton-Andrew Dhafir Jones
• 金额: $ 38.28万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-01 至 2026-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10650399
• 关键词: 3D Print; Affect; Animals; Antibiotics; Bacteria; Candida; Candida albicans; Cells; Cessation of life; Clinical; Communication; Consumption; Development; Devices; Engineering; Food Contamination; Geometry; Hygiene; Infection; Liquid substance; Manufacturer; Mathematics; Medical Device; Meropenem; Microbial Biofilms; Milk; Monitor; Nosocomial Infections; Nutrient; Operative Surgical Procedures; Play; Pseudomonas aeruginosa; Reaction; Rejuvenation; Research; Research Personnel; Risk; Role; Rotation; Sanitation; Stress; Surface; Swimming; System; Temperature; Time; Translating; cost; medical implant; nanoparticle; polymicrobial biofilm; programs; secondary infection; shear stress; sugar; uptake; wasting

Project Summary/Abstract Bacteria in biofilms are more responsible than planktonic cells, for food contamination, infection during cleaning, and are responsible for 80% of all hospital acquired infections, about 1.6 million infections and 80,000 deaths, at an annual cost of $16.8 to $27.2 billion dollars in the US. Treating biofilms on surfaces requires constant cleaning as, for example, biofilms that are able to rejuvenate within 30 minutes after raw milk is introduced to a milk evaporator. Biofilm infections on implanted medical devices make up half of the hospital acquired infections and the primary treat is to remove the device, increasing surgery and increasing risk of secondary infection. Biofilm development and reaction to antibiotics are dependent on the geometry, nutrient, and liquid flow conditions and there are currently few relationships available to translate between one set of conditions and another. This program seeks to create platforms and mathematical relations that will increase the ability of researchers, clinicians and manufacturers, to translate from fundamental studies on biofilms with finite time scales and materials to infections and surfaces. The primary platforms that the proposal seeks to explore are those that can monitor the impact of external stress, like temperature, fluid shear, antibiotics, nanoparticles at each stage of biofilm development and simulate real world conditions. Some of those platforms initially proposed are those that can be made using 3D printers and those that can be easily scaled, like rotating disk systems. The primary mathematical relations that will be proposed are dimensionless numbers that can be used to translate from one experimental apparatus to another. Four dimensionless numbers have been proposed for biofilms and three have been proposed for communication in biofilms, though their use is spare. Part of this proposal seeks to determine what role, if any, sociomicrobiological interactions, play in biofilm persistence. For example, Candida albicans increases meropenem tolerance of Pseudomonas aeruginosa. The use of waste byproducts as a means of interaction will likely be prioritized, like how sugar consumed by Candida may be responsible for the increased tolerance mentioned previously. Finally, this proposal also seeks to determine how biofilm substitutes can be used to study the impacts of external stress, like how fluid shear stress and temperature affect antibiotic and nanoparticle and uptake. While neither clinical nor animal trials are proposed in this study, a participatory dialogue will be conducted between the researchers, clinicians, manufacturers, and engineers, and sanitation and hygiene professionals to ensure that results from this research can be used.

项目概要/摘要 生物膜中的细菌比浮游细胞对食品污染、感染过程中的感染负有更大的责任。 80% 的医院获得性感染（约 160 万例感染）和 美国有 8 万人死亡，每年造成的损失为 16.8 至 272 亿美元。处理表面生物膜 需要持续清洁，例如生物膜能够在生奶后 30 分钟内恢复活力 被引入牛奶蒸发器。植入式医疗器械生物膜感染占医院一半 获得性感染，主要治疗方法是移除装置，增加手术次数并增加感染风险 继发感染。生物膜的形成和对抗生素的反应取决于几何形状、营养物质、 和液体流动条件，目前几乎没有可用于在一组之间进行转换的关系 条件和另一个。该计划旨在创建平台和数学关系，以增加 研究人员、临床医生和制造商将生物膜基础研究转化为 有限的时间尺度和材料对感染和表面的影响。该提案寻求的主要平台 探索的是那些可以监测外部压力影响的技术，如温度、流体剪切力、抗生素、 纳米颗粒在生物膜发育的每个阶段并模拟现实世界的条件。其中一些 最初提出的平台是那些可以使用 3D 打印机制作的平台以及那些可以轻松扩展的平台， 像旋转磁盘系统。将提出的主要数学关系是无量纲的 可用于从一种实验设备转换到另一种实验设备的数字。四无量纲 已为生物膜提出了数字，并为生物膜中的通信提出了三个数字，但 他们的用途是备用的。该提案的一部分旨在确定社会微生物相互作用的作用（如果有的话）， 在生物膜持久性中发挥作用。例如，白色念珠菌可增加假单胞菌对美罗培南的耐受性 铜绿假单胞菌。使用废弃副产品作为互动手段可能会被优先考虑，就像糖如何 念珠菌消耗的可能是前面提到的耐受性增加的原因。最后，这个 该提案还试图确定如何使用生物膜替代品来研究外部压力的影响， 例如流体剪切应力和温度如何影响抗生素和纳米颗粒及其吸收。虽然临床上都没有 本研究中也不提议进行动物试验，因此将在研究人员之间进行参与性对话 研究人员、临床医生、制造商、工程师以及环境卫生和个人卫生专业人员，以确保 可以使用本研究的结果。