Developing platforms for studying the impact of external stresses on multispecies biofilms.

开发用于研究外部压力对多物种生物膜影响的平台。

• 批准号: 10650399
• 负责人: Akhenaton-Andrew Dhafir Jones
• 金额: $ 38.28万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-01 至 2026-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10650399
• 关键词: 3D Print; Affect; Animals; Antibiotics; Bacteria; Candida; Candida albicans; Cells; Cessation of life; Clinical; Communication; Consumption; Development; Devices; Engineering; Food Contamination; Geometry; Hygiene; Infection; Liquid substance; Manufacturer; Mathematics; Medical Device; Meropenem; Microbial Biofilms; Milk; Monitor; Nosocomial Infections; Nutrient; Operative Surgical Procedures; Play; Pseudomonas aeruginosa; Reaction; Rejuvenation; Research; Research Personnel; Risk; Role; Rotation; Sanitation; Stress; Surface; Swimming; System; Temperature; Time; Translating; cost; medical implant; nanoparticle; polymicrobial biofilm; programs; secondary infection; shear stress; sugar; uptake; wasting

Project Summary/Abstract Bacteria in biofilms are more responsible than planktonic cells, for food contamination, infection during cleaning, and are responsible for 80% of all hospital acquired infections, about 1.6 million infections and 80,000 deaths, at an annual cost of $16.8 to $27.2 billion dollars in the US. Treating biofilms on surfaces requires constant cleaning as, for example, biofilms that are able to rejuvenate within 30 minutes after raw milk is introduced to a milk evaporator. Biofilm infections on implanted medical devices make up half of the hospital acquired infections and the primary treat is to remove the device, increasing surgery and increasing risk of secondary infection. Biofilm development and reaction to antibiotics are dependent on the geometry, nutrient, and liquid flow conditions and there are currently few relationships available to translate between one set of conditions and another. This program seeks to create platforms and mathematical relations that will increase the ability of researchers, clinicians and manufacturers, to translate from fundamental studies on biofilms with finite time scales and materials to infections and surfaces. The primary platforms that the proposal seeks to explore are those that can monitor the impact of external stress, like temperature, fluid shear, antibiotics, nanoparticles at each stage of biofilm development and simulate real world conditions. Some of those platforms initially proposed are those that can be made using 3D printers and those that can be easily scaled, like rotating disk systems. The primary mathematical relations that will be proposed are dimensionless numbers that can be used to translate from one experimental apparatus to another. Four dimensionless numbers have been proposed for biofilms and three have been proposed for communication in biofilms, though their use is spare. Part of this proposal seeks to determine what role, if any, sociomicrobiological interactions, play in biofilm persistence. For example, Candida albicans increases meropenem tolerance of Pseudomonas aeruginosa. The use of waste byproducts as a means of interaction will likely be prioritized, like how sugar consumed by Candida may be responsible for the increased tolerance mentioned previously. Finally, this proposal also seeks to determine how biofilm substitutes can be used to study the impacts of external stress, like how fluid shear stress and temperature affect antibiotic and nanoparticle and uptake. While neither clinical nor animal trials are proposed in this study, a participatory dialogue will be conducted between the researchers, clinicians, manufacturers, and engineers, and sanitation and hygiene professionals to ensure that results from this research can be used.

项目摘要/摘要 生物膜中的细菌比浮游细胞更负责，用于食物污染，感染期间 清洁，并负责所有医院收到的感染的80％，大约160万感染和 80,000人死亡，每年的成本为16.8至272亿美元。在表面上处理生物膜 需要持续清洁，例如，生物膜能够在生牛奶后30分钟内恢复活力 被引入牛奶蒸发器。植入医疗设备上的生物膜感染构成了医院的一半 获得的感染和主要治疗是删除设备，增加手术并增加 继发感染。生物膜发育和对抗生素的反应取决于几何，营养素， 和液体流量条件，目前几乎没有关系可以在一组之间翻译 条件和另一个。该计划旨在建立平台和数学关系，以增加 研究人员，临床医生和制造商的能力从与生物膜有关的基本研究转化为 有限的时间尺度和材料到感染和表面。该提案试图的主要平台 探索是可以监测外部压力的影响，例如温度，液体剪切，抗生素， 生物膜开发的每个阶段的纳米颗粒并模拟现实世界的条件。其中一些 最初提出的平台是可以使用3D打印机制造的平台，并且可以轻松缩放的平台， 就像旋转磁盘系统一样。将提出的主要数学关系是无限的 可用于从一个实验设备转换为另一个实验设备的数字。四个无尺寸 已经提出了用于生物膜的数字，并提出了三个用于生物膜的通信 他们的使用是备用的。该提案的一部分旨在确定社会生物学互动的角色（如果有的话） 在生物膜持久性中发挥作用。例如，白色念珠菌增加了假单胞菌的Meropenem耐受性 铜绿。使用废物副产品作为一种互动手段可能会优先考虑，例如糖的方式 念珠菌消耗可能是由于前面提到的公差增加而导致的。最后，这个 提案还试图确定如何使用生物膜替代品来研究外部压力的影响， 像流体剪切应力和温度如何影响抗生素和纳米颗粒以及摄取。虽然都不临床 在本研究中，也没有提出动物试验，也不会进行参与性对话 研究人员，临床医生，制造商和工程师以及卫生和卫生专业人员，以确保 可以使用这项研究的结果。