A Mechanical Testing Machine for Characterizing Biofilms on Experimental Tools

用于表征实验工具上生物膜的机械测试机

基本信息

批准号：
10799118
负责人：
Akhenaton-Andrew Dhafir Jones
金额：
$ 5.97万
依托单位：
DUKE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-09-01 至 2026-06-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10799118
关键词：
3D Print Acids Acne Affect Agar Alginates Animal Model Animals Antibiotics Antimicrobial Resistance Bacteria Bacterial Adhesion Bacterial Infections Behavior Candida Candida albicans Cells Cellulose Cessation of life Chemicals Clinical Communication Consumption Cosmetics DNA Data Debridement Dental Development Devices Divalent Cations Engineering Environment Esters Etiology Excision Exposure to Family suidae Food Contamination Future Gene Transfer Geometry Growth Health Homeostasis Human Hydrogels Hydrolysis Hygiene Immune response Infection International Ligand Binding Liquid substance Machine Learning Manufacturer Mathematics Measures Mechanics Medical Medical Device Membrane Meropenem Metals Methodology Methods Microbial Biofilms Milk Modeling Modification Modulus Monitor Nosocomial Infections Nutrient Operative Surgical Procedures Organism Play Polymers Predatory Behavior Property Proteins Proteomics Pseudomonas aeruginosa Reaction Rejuvenation Research Research Personnel Risk Role Rotation Salts Sanitation Site Skin Skin Tissue Source Staphylococcus aureus Staphylococcus epidermidis Stress Surface Swimming System Temperature Testing Time Tissue Model Tissue constructs Tissues Translating Uncertainty United States Vacuole Work Wound Infection analog assault chemical property cost cystic fibrosis infection cytokine dysbiosis extracellular feature extraction improved in vivo evaluation mechanical properties medical implant metabolomics mouse model nano nanoparticle novel polymicrobial biofilm programs quorum sensing response secondary infection shear stress success sugar tool uptake wasting wound wound closure

项目摘要

Project Summary/Abstract Bacteria in biofilms are more responsible than planktonic cells, for food contamination, infection during cleaning, and are responsible for 80% of all hospital acquired infections, about 1.6 million infections and 80,000 deaths, at an annual cost of $16.8 to $27.2 billion dollars in the US. Treating biofilms on surfaces requires constant cleaning as, for example, biofilms that are able to rejuvenate within 30 minutes after raw milk is introduced to a milk evaporator. Biofilm infections on implanted medical devices make up half of the hospital acquired infections and the primary treat is to remove the device, increasing surgery and increasing risk of secondary infection. Biofilm development and reaction to antibiotics are dependent on the geometry, nutrient, and liquid flow conditions and there are currently few relationships available to translate between one set of conditions and another. This program seeks to create platforms and mathematical relations that will increase the ability of researchers, clinicians and manufacturers, to translate from fundamental studies on biofilms with finite time scales and materials to infections and surfaces. The primary platforms that the proposal seeks to explore are those that can monitor the impact of external stress, like temperature, fluid shear, antibiotics, nanoparticles at each stage of biofilm development and simulate real world conditions. Some of those platforms initially proposed are those that can be made using 3D printers and those that can be easily scaled, like rotating disk systems. The primary mathematical relations that will be proposed are dimensionless numbers that can be used to translate from one experimental apparatus to another. Four dimensionless numbers have been proposed for biofilms and three have been proposed for communication in biofilms, though their use is spare. Part of this proposal seeks to determine what role, if any, sociomicrobiological interactions, play in biofilm persistence. For example, Candida albicans increases meropenem tolerance of Pseudomonas aeruginosa. The use of waste byproducts as a means of interaction will likely be prioritized, like how sugar consumed by Candida may be responsible for the increased tolerance mentioned previously. Finally, this proposal also seeks to determine how biofilm substitutes can be used to study the impacts of external stress, like how fluid shear stress and temperature affect antibiotic and nanoparticle and uptake. While neither clinical nor animal trials are proposed in this study, a participatory dialogue will be conducted between the researchers, clinicians, manufacturers, and engineers, and sanitation and hygiene professionals to ensure that results from this research can be used.

项目概要/摘要生物膜中的细菌比浮游细胞对食品污染、感染过程中的感染负有更大的责任。 80% 的医院获得性感染（约 160 万例感染）和美国有 8 万人死亡，每年造成的损失为 16.8 至 272 亿美元。处理表面生物膜需要持续清洁，例如生物膜能够在生奶后 30 分钟内恢复活力被引入牛奶蒸发器。植入式医疗器械生物膜感染占医院一半获得性感染，主要治疗方法是移除装置，增加手术次数并增加感染风险继发感染。生物膜的形成和对抗生素的反应取决于几何形状、营养物质、和液体流动条件，目前几乎没有可用于在一组之间进行转换的关系条件和另一个。该计划旨在创建平台和数学关系，以增加研究人员、临床医生和制造商将生物膜基础研究转化为有限的时间尺度和材料对感染和表面的影响。该提案寻求的主要平台探索的是那些可以监测外部压力影响的技术，如温度、流体剪切力、抗生素、纳米颗粒在生物膜发育的每个阶段并模拟现实世界的条件。其中一些最初提出的平台是那些可以使用 3D 打印机制作的平台以及那些可以轻松扩展的平台，像旋转磁盘系统。将提出的主要数学关系是无量纲的可用于从一种实验设备转换到另一种实验设备的数字。四无量纲已为生物膜提出了数字，并为生物膜中的通信提出了三个数字，但他们的用途是备用的。该提案的一部分旨在确定社会微生物相互作用的作用（如果有的话），在生物膜持久性中发挥作用。例如，白色念珠菌可增加假单胞菌对美罗培南的耐受性铜绿假单胞菌。使用废弃副产品作为互动手段可能会被优先考虑，就像糖如何念珠菌消耗的可能是前面提到的耐受性增加的原因。最后，这个该提案还试图确定如何使用生物膜替代品来研究外部压力的影响，例如流体剪切应力和温度如何影响抗生素和纳米颗粒及其吸收。虽然临床上都没有本研究中也不提议进行动物试验，因此将在研究人员之间进行参与性对话研究人员、临床医生、制造商、工程师以及环境卫生和个人卫生专业人员，以确保可以使用本研究的结果。

项目成果

期刊论文数量（4）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Heterogenous Biofilm Mass-Transport Model Replicates Periphery Sequestration of Antibiotics in P. aeruginosa PAO1 Microcolonies.

异质生物膜传质模型复制铜绿假单胞菌 PAO1 微菌落中抗生素的外围隔离。

DOI：
10.1101/2023.07.28.551018
发表时间：
2023
期刊：
bioRxiv : the preprint server for biology
影响因子：
0
作者：
Prince,Joshua;Jones3rd,A-AndrewD
通讯作者：
Jones3rd,A-AndrewD

Heterogenous biofilm mass-transport model replicates periphery sequestration of antibiotics in Pseudomonas aeruginosa PAO1 microcolonies.

DOI：
10.1073/pnas.2312995120
发表时间：
2023-11-21
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
影响因子：
11.1
作者：
Prince, Joshua;Jones III, A-Andrew D.
通讯作者：
Jones III, A-Andrew D.

A microtiter peg lid with ziggurat geometry for medium-throughput antibiotic testing and in situ imaging of biofilms.