Genetics and neurobiology of aggression of Betta splendens

芨芨草攻击行为的遗传学和神经生物学

基本信息

批准号：
10731186
负责人：
Pei Yin Shih
金额：
$ 12.5万
依托单位：
COLUMBIA UNIV NEW YORK MORNINGSIDE
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-08-15 至 2025-07-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10731186
关键词：
3D Print Affect Aggressive behavior Amygdaloid structure Animal Behavior Animals Behavior Behavioral Brain region Breeding CRISPR/Cas technology Cessation of life Chromosome Mapping Classification Code Custom Data Development Development Plans Economic Burden Etiology Excitatory Synapse Faculty Fishes Foundations Frequencies Generations Genes Genetic Genetic Variation Genomics Gills Goals Heritability Human Image Individual Individual Differences Infrastructure Inhibitory Synapse Knock-out Knowledge Lung Machine Learning Maintenance Maps Mediating Mentorship Methods Mind Modeling Molds Molecular Mollies Mus Nervous System Neurobiology Neurons Partner in relationship Pathway interactions Pattern Performance Phase Physiological Population Genetics Positioning Attribute Quantitative Trait Loci Recording of previous events Research Research Activity Research Personnel Resource Development Resources Robot Ruta Shapes Signal Transduction Social Behavior Societies Specific qualifier value Statistical Methods Synapses System Techniques Technology Testing Tracer Transgenic Organisms Variant Work autism spectrum disorder base behavior test behavioral response brain behavior career career development erection falls fighting gain of function gene discovery genetic approach genetic variant innovation insight interdisciplinary approach interest neural neural circuit neuroligin 3 neuronal circuitry neuropsychiatry novel novel strategies overexpression siamese fighting fish skills tool trait visual stimulus

项目摘要

Project Summary / Abstract Project description: Aggression is a competitive social behavior that animals use to acquire resources and protect kin. While genetic variation is known to contribute to individual differences in aggression, we know very little about the specific genes involved and their mechanisms. To discover genes that regulate aggression, the applicant will exploit the unique features of the Siamese fighting fish Betta splendens (betta). Highly aggressive fighting betta have been generated through a long history of artificial breeding. Their aggressive behaviors are easily triggered by visual stimuli, and their intense fighting continues until retreat or death. The applicant proposes to use these fighting fish, along with their unselected, less aggressive wild counterparts, to expand our knowledge of genetic and neuronal bases underlying aggression. In Aim 1 (K99), the applicant will characterize the behavioral differences between wild and fighting betta during fights by developing a novel behavioral test and utilizing machine-learning based tools for behavioral analyses. In Aim 2 (K99), the applicant will discover aggression-associated variants that contribute to the behavioral differences with complementary mapping methods and genetic tools for functional perturbation. Finally, in Aim 3 (R00), the applicant will probe how the mapped genes influence the nervous system and mediate aggression using neuronal imaging and circuit tracing tools. Candidate and career goals: The applicant’s broad research interests involve understanding how genetic variation shapes the nervous system to modulate behaviors. The proposed research activities will build upon the applicant’s strong background in genetics and genomics skillsets with imaging and circuit tracing techniques, enabling the interdisciplinary approach necessary to tackle the applicant’s highly innovative research questions. The proposed project will also lay the foundation for the applicant’s long-term goal as an independent researcher who brings computational, genetic, and neurobiological fields together to create bold new approaches for understanding social behavior. Career development plan: The K99 phase of this work will be done in Columbia’s Zuckerman Mind Brain Behavior Institute (ZMBBI) under the mentorship of Drs. Andrés Bendesky and Franck Polleux. In addition, the candidate has assembled an extraordinary team of advisors and collaborators, consisting of Drs. Molly Przeworski, Carolyn (Lindy) McBride, Vanessa Ruta, and David Manuel Flores Gonçalves. The applicant’s research and transition to independence will benefit from this strong mentorship team, the state-of-the-art facilities and infrastructure at ZMBBI, and numerous Professional Development resources offered through ZMBBI and Columbia.

项目摘要 /摘要项目描述：侵略是一种竞争性的社会行为，动物用来获取资源和保护亲戚。虽然已知遗传变异会导致侵略性的个体差异，但我们非常了解关于涉及的特定基因及其机制的知识很少。为了发现调节侵略性的基因，申请人将利用暹罗战鱼Betta Splendens（Betta）的独特功能。高度侵略性战斗斗式已经通过人工育种的悠久历史产生。他们的侵略性行为是视觉刺激很容易触发，他们的激烈战斗一直持续到撤退或死亡为止。申请人提议使用这些战鱼以及未选择的，侵略性的野生对应物的提议，以扩大我们对侵略性基础的遗传和神经元基础的了解。在AIM 1（K99）中，适用的将通过发展小说来表征战斗过程中野性与战斗斗斗之间的行为差异行为测试并利用基于机器学习的工具进行行为分析。在AIM 2（K99）中申请人将发现与行为差异有关的积极相关变体完全映射功能扰动的方法和遗传工具。最后，在AIM 3（R00）中申请人将探测映射的基因如何影响神经系统并使用侵略性神经元成像和电路跟踪工具。候选人和职业目标：申请人的广泛研究兴趣包括了解遗传变异塑造神经系统以调节行为。拟议的研究活动将基于申请人具有成像和电路跟踪的遗传学和基因组技能的强大背景技术，使能够解决申请人高度创新的跨学科方法研究问题。拟议的项目还将为申请人的长期目标奠定基础将计算，遗传和神经生物学领域融合在一起以创建大胆的独立研究人员理解社会行为的新方法。职业发展计划：这项工作的K99阶段将在哥伦比亚的Zuckerman Mind Brain中完成行为研究所（ZMBBI）在Drs的心态下。安德烈斯·本德斯基（AndrésBendesky）和弗兰克·波勒（Franck Polleux）。另外，候选人组建了一个由DRS组成的非凡团队。莫莉 Przeworski，Carolyn（Lindy）McBride，Vanessa Ruta和David Manuel FloresGonçalves。申请人的对独立的研究和过渡将受益于这个强大的精明团队，即最先进的 ZMBBI的设施和基础设施以及提供的众多专业发展资源 ZMBBI和哥伦比亚。