Acoustically-driven optical-interferometric microscope for cell characterization

用于细胞表征的声学驱动光学干涉显微镜

• 批准号: 10322456
• 负责人: Brian W Anthony
• 金额: $ 19.39万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-01-01 至 2022-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10322456
• 关键词: 3-Dimensional; Acoustics; Address; Alginates; Animals; Apoptosis; Atomic Force Microscopy; Automobile Driving; Benchmarking; Biological; Biological Assay; Biological Markers; Biology; Biomechanics; Biopolymers; Breast; Cancerous; Cardiac Myocytes; Cell Culture Techniques; Cell Line; Cell membrane; Cell physiology; Cells; Chemicals; Complex; Cultured Cells; Discrimination; Embryonic Development; Environment; Erythrocytes; Eukaryota; Excision; Extracellular Matrix; Flow Cytometry; Frequencies; Geometry; Goals; Hematological Disease; Heterogeneity; Human; Hypertrophy; Image; Inflammation; Lead; Literature; MCF10A cells; MCF7 cell; Malaria; Malignant Neoplasms; Measurement; Measures; Mechanical Stress; Mechanics; Medicine; Membrane; Methods; Microscope; Microscopic; Microscopy; Modeling; Molecular; Morphology; Motion; Motor; Muscular Dystrophies; Noise; Nuclear Envelope; Optics; Pathologic Processes; Pathology; Patients; Pharmaceutical Preparations; Phase; Physiological; Physiological Processes; Physiology; Play; Population; Process; Property; Reporting; Resolution; Rheology; Role; Sampling; Sickle Cell Anemia; Signal Transduction; Specificity; Subcellular structure; System; Time; Tissues; Transducers; Variant; cancer surgery; chronic wound; clinical translation; design; detection limit; disease diagnosis; flexibility; hydroxyurea; improved; in vivo; instrument; matrigel; mechanical force; mechanical properties; migration; nanoscale; novel; response; stem cell differentiation; sudden cardiac death; tool; transmission process; tumor progression; vaso-occlusive crisis

Abstract The goal of this proposal to develop a first of its kind acoustically driven quantitative phase microscopy (QPM) rheology system. Mechanical forces play an important role in physiology and pathology. Mechanical, electrical, and chemical phenomena govern all processes in cellular systems such as signaling, differentiation, migration, and apoptosis. Cellular mechanics play important roles in normal physiological processes but perhaps more importantly in numerous abnormal pathological processes. Mechanical environment have been demonstrated to regulate stem cell differentiation; embryo development is guided by many mechanical clues. In pathological processes, stiffening of red blood cell membrane is a factor in driving vaso-occlusive crisis in sickle cell disease and malaria patients. Mechanical forces dysregulation can lead to hypertrophy of cardiomyocytes that can cause sudden cardiac death, most commonly in young patients. Inflammation is known to be regulated by mechanical factors and is related to difficulties in treating chronic wounds. The stiffness of extracellular matrix environment is important in regulating cancer progression and the measurement of cell/tissue mechanical properties has been proposed as a way to identify resection margins during cancer surgery. Mechanopharmacology is also known to modulate cellular drug responses. The importance of cellular biomechanics is well recognized; however, the ability to investigate cellular scale mechanical factors in biology and medicine is limited by the available measurement tools.

抽象的 该提案的目的是开发出一种声学驱动的定量相显微镜（QPM）流变学 系统。机械力在生理和病理学中起重要作用。机械，电和化学 现象控制了细胞系统中的所有过程，例如信号传导，分化，迁移和凋亡。细胞 力学在正常的生理过程中起着重要的作用，但更重要的是在许多异常中 病理过程。已经证明机械环境可以调节干细胞分化。胚胎 发展受许多机械线索的指导。在病理过程中，红细胞膜的僵硬是一个 在镰状细胞疾病和疟疾患者中推动血管熟悉的危机的因素。机械力失调可以引导 对于可能导致心脏猝死的心肌细胞肥大，最常见于年轻患者。炎 已知由机械因素调节，与治疗慢性伤口的困难有关。刚度 细胞外基质环境对于调节癌症进展和细胞/组织的测量很重要 已经提出了机械性能，以识别癌症手术期间切除缘的一种方式。 还已知机械药理学可以调节细胞药物反应。细胞生物力学的重要性是 公认；但是，研究生物学和医学中细胞尺度机械因素的能力受到限制 可用的测量工具。